Magnetic Microcarriers with Accurate Localization and Proliferation of Mesenchymal Stem Cell for Cartilage Defects Repairing

Abstract

Magnetic biomaterials are widely used in the field of tissue engineering because of their functions such as drug delivery and targeted therapy. In this study, a magnetically responsive composite microcarrier was prepared through in situ polymerization of dopamine with Fe3O4 (MS) to form a complex. The magnetic composite microcarriers are paramagnetic and have certain magnetic responsiveness, suitable pore size porosity for cell growth, and good blood compatibility and biocompatibility. The bone marrow mesenchyml stem cells (BMSCs) were cultured on magnetic composite microcarriers, and a static magnetic field (SMF) was applied. The results showed that BMSCs adhered to the microcarriers proliferated under the action of horizontal and vertical forces. Magnetic composite microcarriers loaded with BMSCs were implanted into the SD rat model of cartilage defect, and a magnet was added to the operative side. After 12 weeks, cartilage regeneration was observed. The results of gross observation and histological immunostaining 1 month, 2 months, and 3 mounths after operation showed that the magnetic composite microcarriers of loaded cells promoted the early maturation of cartilage and collagen secretion, and the effect of cartilage repair was significantly better than that of the control group. Gait analysis showed that implanting magnetic composite microcarriers loaded with stem cells can reduce postoperative pain and promote limb recovery in SD rats. In conclusion, this study suggests that magnetic composite microcarriers are promising tissue-engineered scaffolds for cartilage regeneration and repair.

A bibliometric analysis of intra-articular injection therapy for knee osteoarthritis from 2012 to 2022

“…As a promising DNA nanomaterial to improve the regenerative microenvironment, tetrahedral framework nucleic acid can be absorbed into bone marrow mesenchymal stem cells and promote the proliferation and chondrogenic differentiation of bone marrow mesenchymal stem cells. [55] Six months after a single injection of AD-MSCs, WOMAC scores had significantly improved. Six months later, the WOMAC score of the control group did not change appreciably.…”

Stimuli-responsive microcarriers and their application in tissue repair: A review of magnetic and electroactive microcarrier

“… Tissue Materials Degradation Property Synthetic Method Modification Method Cell/Growth Factor/Drug MF Type In Vivo Ref. 1 Cartilage PLGA Biodegradable W–O–W Emulsion & Template Method Ferumoxytol & Chitosan Electrostatic interaction TGF-β1 & MSCs SMF / [ 36 ] 2 CS Biodegradable Antiphase suspension & Thermally induced phase separation Fe 3 O 4 NPs PDA Adhesion BMSCs SMF SD rat [ 41 ] 3 ECM Biodegradable Decellularization Fe 3 O 4 NPs Dip-Coating hBMSCs RMF SD rat [ 193 ] 4 SC spheroid Biodegradable SC Spheroid Culture Fe 3 O 4 NPs (dextran) Co-culture Mouse MSCs EMF / [ 194 ] 5 CS Biodegradable Freeze-drying & Femtosecond-pulsed UV laser cutting Ferumoxytol & Col-I Electrostatic & van der Waals interactions hADMSCs EMF …”

“…(C) The magnetic-responsive liquified capsules encapsulating stem cells and collagen II/TGF-β3 microparticles to construct closed chondromimetic environment [ 37 ]. (D) Functionalized magnetic microcarrier CS/PDA@MS for cartilage defect repair [ 41 ]. (E) Janus microcarriers with natural bone healing-mimetic sequential release ability for bone regeneration [ 45 ] (F) Magnetic BMSCs exosomes promote osteogenesis and angiogenesis by the enrichment of miR-1260a [ 46 ].…”

Recent advances in 3D bioprinted cartilage-mimicking constructs for applications in tissue engineering

“…The regeneration and repair of defective cartilage tissue has long been a challenge due to insufficient blood supply. Current therapeutic methods for cartilage diseases include artificial scaffolds [ 1 , 2 ], microfractures [ 3 , 4 ], autologous cartilage transplantation [ 5 , 6 ], stem cell therapy [ 7 ], and more [ 8 ]. While these operations have achieved some curative effects, they still have some problems, such as donor injury, long treatment cycles, prosthetic infection, and unsatisfactory curative efficacy.…”