Magnetic nanoparticle mediated-gene delivery for simpler and more effective transformation of <i>Pichia pastoris</i>

Yildiz, Seyda; Solak, Kübra; Acar, Melek; Mavi, Ahmet; Ünver, Yağmur

doi:10.1039/d1na00079a

Cited by 9 publications

(2 citation statements)

References 49 publications

(70 reference statements)

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“…In vitro transfection experiments of gene vectors, fluorescence is generally used as a marker of cell transfection to determine the transfection efficiency of gene vectors 44 . In this study, Fe 3 O 4 @PDA@PEI/DNA polyplexes were prepared using plasmids containing GFP expression genes.…”

Section: Resultsmentioning

confidence: 99%

“…In vitro transfection experiments of gene vectors, fluorescence is generally used as a marker of cell transfection to determine the transfection efficiency of gene vectors. 44 In this study, Fe 3 O 4 @PDA@PEI/DNA polyplexes were prepared using plasmids containing GFP expression genes. The transfection efficiency was studied by examining the expression efficiency of GFP in cells after coculture with the Fe 3 O 4 @PDA@PEI/DNA polyplexes.…”

Section: In Vitro Gene Transfectionmentioning

confidence: 99%

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Preparation of PEI‐modified nanoparticles by dopamine self‐polymerization for efficient DNA delivery

Liu

Yang

Liu

et al. 2022

Biotech and App Biochem

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Achieving efficient and safe gene delivery is of great significance to promote the development of gene therapy. In this work, a polydopamine (PDA) layer was coated on the surface of Fe3O4 nanoparticles (NPs) by dopamine (DA) self‐polymerization, and then magnetic Fe3O4 NPs were prepared by the Michael addition between amino groups in polyethyleneimine (PEI) and PDA. The prepared Fe3O4 NPs (named Fe3O4@PDA@PEI) were characterized by Fourier transform infrared (FTIR), atomic force microscopy (AFM), and scanning electron microscopy (SEM). As an efficient and safe gene carrier, the potential of Fe3O4@PDA@PEI was evaluated by agarose gel electrophoresis, 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide assay, fluorescence microscopy, and flow cytometry. The results show that the Fe3O4@PDA@PEI NPs are stable hydrophilic NPs with a particle size of 50–150 nm. It can efficiently condense DNA at low N/P ratios and protect it from nuclease degradation. In addition, the Fe3O4@PDA@PEI NPs have higher safety than PEI. Further, the Fe3O4@PDA@PEI/DNA polyplexes could be effectively absorbed by cells and successfully transfected and exhibit higher cellular uptake and gene transfection efficiency than PEI/DNA polyplexes. The findings indicate that the Fe3O4@PDA@PEI NPs have the potential to be developed into a novel gene vector.

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Section: Resultsmentioning

confidence: 99%

Section: In Vitro Gene Transfectionmentioning

confidence: 99%