Magnetic Nanoparticles-Based Extraction and Verification of Nucleic Acids from Different Sources

Abstract

In many molecule biology and genetic technology studies, the amount of available DNA can be one of the important criteria for selecting the samples from different sources. Compared with those genomic DNA methods using organic solvents or other traditional commercial kits, the method based on magnetic nanoparticles (MNPs) and adsorption technology has many remarkable advantages like being time-saving and cost effective without the laborious centrifugation or precipitation steps, and more importantly it has the great potential and especially suitable for automated DNA extraction and up-scaling. In this paper, the extraction efficiency of genomic nucleic acids based on magnetic nanoparticles from four different sources including bacteria, yeast, human blood and virus samples are compared and verified. After measurement and verification of the extracted genomic nucleic acids, it was shown that all these genomic nucleic acids extracted using the MNPs method can be of high yield and be available for next molecule biological steps.

Extraction of human genomic DNA from whole blood using a magnetic microsphere method

“…For magnetic microspheres to be marketable, they need to have a uniform particle size, a strong magnetic response, good stability, and a surface rich in active functional groups. [1][2][3][4][5][6] How to improve the binding efficiency and specificity of biological macromolecules on the surfaces of magnetic microspheres and expand their scope of application has been the focus of intense research interest.…”

“…However, extracting genomic DNA by traditional methods is a timeconsuming process, and phenol and chloroform are toxic reagents that endanger health. [2][3][4][5][6] Further, traditional methods, such as phenol extraction, isopropanol precipitation, the formamide lysate method, nonorganic solvent extraction, and glass particle adsorption, have been found to be ineffective for extracting genomic DNA from trace, dried, and frozen blood. Therefore, it is necessary to find a more convenient and efficient method for obtaining human genomic DNA.…”

Characterization and Functional Analysis of Viral siRNAs in Tomato Infected with <I>Cucumber Mosaic Virus</I>

“…The quantity and purity of total plant RNAs were assayed with the NanoDrop ND-1000 spectrophotometer (NanoDrop Technologies, Wilmington, USA) at 260/280 nm (ratio > 2.0). [26][27][28][29][30][31][32][33] The RNAs extracted from mock-or virus-treated tomato plants were named as Group A and group B, and were used for highthroughput sequencing and real-time PCR analysis.…”

Chelerythrine and Fe3O4 Loaded Multi-Walled Carbon Nanotubes for Targeted Cancer Therapy

“…Functionalization of the CNTs with magnetic Fe 3 O 4 nanoparticles (NPs) has been suggested to be very important in developing the targeting ability of the oxidized-CNTs. The multifunctional properties of the Fe 3 O 4 NPs, such as small size, high magnetic responsivity and low toxicity, [34][35][36][37][38][39][40][41][42][43] can be used to increase the accumulation of drugs in pathological sites, thereby reducing the non-specific toxicity and some other adverse side-effects. We therefore decorated the MWNTs with Fe 3 O 4 NPs to ensure a strong magnetic responsiveness and provide the targeted property for various applications.…”