Magnetic-particle-based, ultrasensitive chemiluminescence enzyme immunoassay for free prostate-specific antigen

Liu, Ruping; Wang, Cheng; Quan, Jiang; Zhang, Wei; Yue, Zhao; Li, Guohua

doi:10.1016/j.aca.2013.09.050

Cited by 58 publications

(27 citation statements)

References 20 publications

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“…Furthermore, the CMIA method anchors antibodies via magnetic microparticles, a fact that makes this analytical method advantageous compared to ELISA assays. In particular, CMIA requires less dose of immunoreagents and time, while it provides a higher dose hook effect and bioactivity of immunoreagents as well as higher sensitivity, better reproducibility, and stability (Zhang et al 2012;Liu et al 2013).…”

Section: Blood and Tissue Collection And Preparationmentioning

confidence: 99%

Plasma from exercised rats administered to sedentary rats induces systemic and tissue inflammation

Γουτιάνος

Veskoukis

Tzioura

et al. 2016

Physiological Reports

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Recent studies have consistently supported the active role of blood in mediating biochemical and physiological tissue adaptations. However, no study has investigated the possible contribution of circulating factors in an exercise setting. The aim of the study was to investigate the role of circulating factors in exercise adaptations by chronically administering to sedentary animals blood plasma collected from acutely exercised animals. Phase 1: Blood plasma was collected from rats that swam to exhaustion and from sedentary rats. Phase 2: Other rats were divided into two groups (n = 20 per group): the first group involved rats that were injected intravenously with blood plasma originating from rats that previously swam to exhaustion, the second group consisted of rats that were injected intravenously with blood plasma originating from sedentary rats. Tail‐vein injections (2 mL/kg) were performed daily for 21 consecutive days. Inflammatory markers (C‐reactive protein, interleukins‐1α, 2, 6, 8, 10 and tumor necrosis factor‐a) were measured in blood plasma, muscle, and adipose tissue. Sedentary rats administered with plasma from exercised rats had significantly higher levels in all inflammatory markers measured in blood, skeletal muscle and adipose tissue, compared to the sedentary rats administered with resting plasma. Our data demonstrate that administration of “exercised” blood to sedentary rats induced inflammation in plasma, muscle and adipose tissue. Exercise adaptations are not solely due to intrinsic processes in muscle or adipose tissue. Blood factors also play a crucial role in mediating signals for tissue adaptations.

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Section: Blood and Tissue Collection And Preparationmentioning

confidence: 99%

Plasma from exercised rats administered to sedentary rats induces systemic and tissue inflammation

Γουτιάνος

Veskoukis

Tzioura

et al. 2016

Physiological Reports

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“…Other immunoreaction‐based methods, such as surface plasmon resonance (SPR) or surface‐enhanced Raman scattering (SERS), required complicated and expensive instruments that made them difficult in practical diagnosis screening. Chemiluminescent immunoassay (CLIA) including chemiluminescence enzyme immunoassay (CLEIA) and electrochemiluminescence have been developed and adopted in routine clinical analysis for the high sensitivity determination of TPSA or FPSA. However, almost all of the aforementioned methods are either only for the detection of TPSA or only for the measurement of FPSA, which usually results in unmatched F/T ratios .…”

Section: Introductionmentioning

confidence: 99%

Dual‐labeled chemiluminescence enzyme immunoassay for simultaneous measurement of total prostate specific antigen (TPSA) and free prostate specific antigen (FPSA)

et al. 2017

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The specificity for early diagnostic of prostate-specific antigen (PSA) is low because the current technology mostly allows the detection of only one biomarker at one time. In this work, a dual- The proposed method presented the sensitivity of 0.03 ng ml −1 and 0.05 ng ml −1 for FPSA and TPSA respectively, with low cross-reactivities. Compared with the results from commercial chemiluminescent kits there was good correlation, indicating that this established method could be used to simultaneously to measure the concentrations of FPSA and TPSA in one serum sample and also could greatly facilitate the early diagnosis for PCa in clinical practice. KEYWORDS chemiluminescent enzyme immunoassay (CLEIA), dual-labeled, free prostate specific antigen (FPSA), simultaneous measurement, total prostate specific antigen (TPSA)

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“…1,2 In blood, total PSA (tPSA) is composed of free PSA (fPSA) and complexed PSA (cPSA), and the ratio of fPSA and tPSA was found to be a more sensitive marker of cancer than tPSA. 3,4 Over the past years, several analytical methods have been developing in this area of the serum determination of PSA, including the enzyme-linked immunosorbent assay (ELISA), 5 radioimmunoassay (RIA), 6 Time-resolved fluoroimmunoassay (TRFIA), 7 chemiluminescence immunoassay (CLIA), 8 chemiluminescent enzyme immunoassay (CLEIA), 9 and electro chemiluminescence immunoassay (ECLIA). 10 Here we describe a combination of one-step dual-label TRFIA and magnetic beads to establish a new immunoassay method for the simultaneous detection of fPSA and tPSA in serum with monoclonal antibodies (mAb) labeled with stable fluorescent chelates of europium (Eu) and samarium (Sm).…”

Section: Introductionmentioning

confidence: 99%

“…Over the past years, several analytical methods have been developing in this area of the serum determination of PSA, including the enzyme‐linked immunosorbent assay (ELISA), radioimmunoassay (RIA), Time‐resolved fluoroimmunoassay (TRFIA), chemiluminescence immunoassay (CLIA), chemiluminescent enzyme immunoassay (CLEIA), and electro chemiluminescence immunoassay (ECLIA) …”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of free and total prostate‐specific antigen by a magnetic particle‐based time‐resolved fluoroimmunoassay

Zhou¹,

Zhang²,

et al. 2017

Clinical Laboratory Analysis

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Magnetic-particle-based, ultrasensitive chemiluminescence enzyme immunoassay for free prostate-specific antigen

Cited by 58 publications

References 20 publications

Plasma from exercised rats administered to sedentary rats induces systemic and tissue inflammation

Plasma from exercised rats administered to sedentary rats induces systemic and tissue inflammation

Dual‐labeled chemiluminescence enzyme immunoassay for simultaneous measurement of total prostate specific antigen (TPSA) and free prostate specific antigen (FPSA)

Simultaneous determination of free and total prostate‐specific antigen by a magnetic particle‐based time‐resolved fluoroimmunoassay

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