Magnetic resonance imaging monitoring dual-labeled stem cells for treatment of mouse nerve injury

Li, Kangan; Qin, Jining; Wang, Xifu; Xu, Yanhong; Shen, Zhe; Lu, Xinwu; Zhang, Guixiang

doi:10.1016/j.jcyt.2013.03.009

Cited by 19 publications

(17 citation statements)

References 36 publications

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“…21 GFP has been widely used as an excellent fluorescent marker, which can be used to verify the presence of living stem cells and eliminate the interference from hemorrhaged and dead cells. 22 After GFP-MSCs were transplanted in a rat model of ischemic stroke, serial MRI was performed to track the long-term distribution and migration of grafted cells. GFP fluorescence was used as image labels to detect the survival and differentiation of grafted cells, and then to verify the findings of MRI (Figure 1).…”

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confidence: 99%

The long-term fate of mesenchymal stem cells labeled with magnetic resonance imaging-visible polymersomes in cerebral ischemia

Duan

Wang

et al. 2017

IJN

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Understanding the long-term fate and potential mechanisms of mesenchymal stem cells (MSCs) after transplantation is essential for improving functional benefits of stem cell-based stroke treatment. Magnetic resonance imaging (MRI) is considered an attractive and clinically translatable tool for longitudinal tracking of stem cells, but certain controversies have arisen in this regard. In this study, we used SPION-loaded cationic polymersomes to label green fluorescent protein (GFP)-expressing MSCs to determine whether MRI can accurately reflect survival, long-term fate, and potential mechanisms of MSCs in ischemic stroke therapy. Our results showed that MSCs could improve the functional outcome and reduce the infarct volume of stroke in the brain. In vivo MRI can verify the biodistribution and migration of grafted cells when pre-labeled with SPION-loaded polymersome. The dynamic change of low signal volume on MRI can reflect the tendency of cell survival and apoptosis, but may overestimate long-term survival owing to the presence of iron-laden macrophages around cell graft. Only a small fraction of grafted cells survived up to 8 weeks after transplantation. A minority of these surviving cells were differentiated into astrocytes, but not into neurons. MSCs might exert their therapeutic effect via secreting paracrine factors rather than directing cell replacement through differentiation into neuronal and/or glial phenotypes.

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confidence: 99%

The long-term fate of mesenchymal stem cells labeled with magnetic resonance imaging-visible polymersomes in cerebral ischemia

Duan

Wang

et al. 2017

IJN

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“…Previously, MR imaging has been successfully used to localize stem cells and to track their persistence and migration over time in animal models of stroke (Lu et al, 2013), injury (Li et al, 2013), degenerative diseases (Moraes et al, 2012) of CNS. Peldschusl (Peldschus et al, 2007) detected single superparamagnetic iron oxide nanoparticle(SPIO)-labeled MSCs using 3-T MR and proposed that the quantitative analysis of labeled stem cells was feasible.…”

Section: Introductionmentioning

confidence: 99%

Efficient In Vitro Labeling Rabbit Bone Marrow-Derived Mesenchymal Stem Cells with SPIO and Differentiating into Neural-Like Cells

Zhang¹,

Li²,

Li³

et al. 2014

Molecules and Cells

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Mesenchymal stem cells (MSCs) can differentiate into neural cells to treat nervous system diseases. Magnetic resonance is an ideal means for cell tracking through labeling cells with superparamagnetic iron oxide (SPIO). However, no studies have described the neural differentiation ability of SPIO-labeled MSCs, which is the foundation for cell therapy and cell tracking in vivo. Our results showed that bone marrow-derived mesenchymal stem cells (BM-MSCs) labeled in vitro with SPIO can be induced into neural-like cells without affecting the viability and labeling efficiency. The cellular uptake of SPIO was maintained after labeled BM-MSCs differentiated into neural-like cells, which were the basis for transplanted cells that can be dynamically and non-invasively tracked in vivo by MRI. Moreover, the SPIO-labeled induced neural-like cells showed neural cell morphology and expressed related markers such as NSE, MAP-2. Furthermore, whole-cell patch clamp recording demonstrated that these neural-like cells exhibited electrophysiological properties of neurons. More importantly, there was no significant difference in the cellular viability and [Ca2+]i between the induced labeled and unlabeled neural-like cells. In this study, we show for the first time that SPIO-labeled MSCs retained their differentiation capacity and could differentiate into neural-like cells with high cell viability and a good cellular state in vitro.

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“…Iron oxide-based nanoparticles with a size of 6-100 nm have been widely used in numerous biomedical applications. Additionally, the morphology and dispersity of nanoparticles also strongly affects their magnetic and biological properties [19,24,25,29,47,48]. In our team's previous work, we synthesized spherical water-soluble magnetic iron oxide nanoparticles with a size of 6.5 nm using a one-step hydrothermal approach.…”

Section: Discussionmentioning

confidence: 99%

“…MRI probes for biomolecular recognition are of great importance in the fields of chemistry, biology, medical sciences and biotechnology. Superparamagnetic iron oxide nanoparticle (SPIO)-based molecular MRI probes have been used to study biological processes [3,[26][27][28][29][30][31]. In summary, MRI is the best potential tool for PNI molecular imaging.…”

Section: Introductionmentioning

confidence: 99%

“…Using cellular MRI, Dr. Shen and coworkers tracked mesenchymal stem cells (MSCs) labelled with gadolinium-diethylene triamine penta-acetic acid (Gd-DTPA) and tetramethyl rhodamine-conjugated linear polyethylenimine derivative (PEI-FluoR) in grafts after transplantation in experimental PNI [30]. Our research group member, Dr. Li, led a study labelling adipose-derived stem cells (ADSCs) with SPIO and then implanted the labelled cells into the murine sciatic nerve conduits; the ADSCs were detected with a clinically available 3.0 T MRI scanner [29]. In a stroke mice model, Dr. Gauberti and colleagues revealed a dynamic inflammatory penumbra after strokes using ultra-sensitive and multi-parametric molecular MRI [32].…”

Section: Introductionmentioning

confidence: 99%

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In vivo targeted peripheral nerve imaging with a nerve-specific nanoscale magnetic resonance probe

Zheng

Han

et al. 2014

Medical Hypotheses

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Magnetic resonance imaging monitoring dual-labeled stem cells for treatment of mouse nerve injury

Cited by 19 publications

References 36 publications

The long-term fate of mesenchymal stem cells labeled with magnetic resonance imaging-visible polymersomes in cerebral ischemia

The long-term fate of mesenchymal stem cells labeled with magnetic resonance imaging-visible polymersomes in cerebral ischemia

Efficient In Vitro Labeling Rabbit Bone Marrow-Derived Mesenchymal Stem Cells with SPIO and Differentiating into Neural-Like Cells

In vivo targeted peripheral nerve imaging with a nerve-specific nanoscale magnetic resonance probe

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