Magnetic tweezers for manipulation of magnetic particles in single cells

Ebrahimian, Haleh; Giesguth, Miriam; Dietz, Karl‐Josef; Reiss, Günter; Herth, Simone

doi:10.1063/1.4865088

Cited by 22 publications

(16 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A similar strategy has also been used with nanoparticles inside cells to regulate the nucleation and assembly of cytoskeletal proteins . However, these methods do not allow control of signaling with single‐cell precision because the aggregation of magnetic nanoparticles cannot be controlled locally unless a complex apparatus, such as a magnetic tweezer, is used to generate localized magnetic fields …”

Section: Figurementioning

confidence: 99%

Remote Control of T Cell Activation Using Magnetic Janus Particles

Lee

2016

Angewandte Chemie

View full text Add to dashboard Cite

We report a strategy for using magnetic Janus microparticles to control the stimulation of T cell signaling with single-cell precision. To achieve this, we design Janus particles that are magnetically responsive on one hemisphere and stimulatory to T cells on the other side. By manipulating the rotation and locomotion of Janus particles under an external magnetic field, w e control the orientation of the particle-cell recognition and thereby initiation of T cell activation. This study demonstrates a step towards employing anisotropic material properties of Janus particles to control single cell activities without the need of complex magnetic manipulation devices. KeywordsJanus particle; magnetic properties; nanotechnology; colloids; Immunology Cells sense their environment and signal one another via receptor-ligand recognition. The binding of ligands to specialized cell surface receptors activates intracellular signaling pathways that determine the behavior of cells. Our ability to regulate these signaling events is essential for not only fundamental understanding of cell biology, but also engineering of cell functions for treating diseases. [1] Among the various strategies for controlling cell signaling with external stimuli, magnetic manipulation is widely used owing to its unique advantages, such as the precise control over the intensity and direction of magnetic forces as well as minimal damage to living cells, in comparison to optical techniques. [1a, 2] Rotating magnetic field has been used to twist magnetic nanoparticles to control the opening of ion channels, [3] measure the mechanical properties of the cell cytoplasm and cytoskeleton, [4] and probe the mechanotransduction of cell surface receptors. [5] Local heating of magnetic nanoparticles has also been exploited to activate temperature-sensitive ion channels in neuron cells. [1a] A few groups used the aggregation of magnetic nanoparticles as a switch to activate cell signaling. [2a-c, 6] In those studies, magnetic nanoparticles were coated with ligands specific for a target signaling pathway, [2a, 2c, 6a, 6b] such as the apoptosis of tumor cells [6d] or immune cell stimulation. [2b, 6c] When nanoparticles aggregated under the influence of a magnetic field, they induced the clustering of the membrane receptors to * yy33@indiana.edu.Supporting information for this article is given via a link at the end of the document. HHS Public Access Author ManuscriptAuthor Manuscript Author ManuscriptAuthor Manuscript which they were bound, which triggered the desired cell responses. A similar strategy has also been used with nanoparticles inside cells to regulate the nucleation and assembly of cytoskeleton proteins. [7] However, these methods do not allow control of signaling with single-cell precision, because the aggregation of magnetic nanoparticles cannot be controlled locally, unless a complex apparatus such as magnetic tweezer is used to generate localized magnetic fields. [8] Here, we present a strategy that employs magnetic Janus microparti...

show abstract

Section: Figurementioning

confidence: 99%

Remote Control of T Cell Activation Using Magnetic Janus Particles

Lee

2016

Angewandte Chemie

View full text Add to dashboard Cite

show abstract

“…This spatiotemporal control was achieved by using simple hand-held magnets, in contrast to studies that relied on the use of magnetic tweezers for localized control of individual cells. [8] We also demonstrate the generality of this approach by using Janus rods. Our group has shown previously that the anisotropic presentation of ligands on Janus particles changes the strength of T cell activation.…”

mentioning

confidence: 98%

“…[7] However, these methods do not allow control of signaling with single-cell precision, because the aggregation of magnetic nanoparticles cannot be controlled locally, unless a complex apparatus such as magnetic tweezer is used to generate localized magnetic fields. [8] …”

mentioning

confidence: 99%

“…[7] However,t hese methods do not allow control of signaling with single-cell precision because the aggregation of magnetic nanoparticles cannot be con-trolled locally unless acomplex apparatus,such as amagnetic tweezer,i sused to generate localized magnetic fields. [8] Herein, we present astrategy that employs magnetic Janus microparticles to remotely control the stimulation of single T cells with only hand-held magnets.Tcell activation is acritical step in cancer immunotherapy,i nw hich ac ancer patients own Tc ells are stimulated, either in vivo or in vitro,t o recognize and attack cancer cells. [9] Being able to remotely control which Tcells to stimulate and switch the cell response on and off is an attractive strategy for the precise administration of the cancer therapy,b ut it remains ag rand challenge.I nt his study,w ea chieved the remote control of activation of single Tcells by exploiting the unique magnetic response of Janus particles.I no ur experimental design, one side of these particles was coated with at hin film of magnetically responsive materials,s ot hat their orientation and locomotion can be controlled simultaneously.T he Janus particles are also biologically anisotropic in that they display stimulatory ligand only on one hemisphere for Tc ell activation.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Remote Control of T Cell Activation Using Magnetic Janus Particles

Lee

2016

Angew Chem Int Ed

View full text Add to dashboard Cite

show abstract

“…To meet the purpose of manipulation cells at single-cell-level by using magnetic tweezers to manipulate the magnetic particles in the cells [59]. In order to fabricate a microstructure that can be powered and controlled wirelessly in fluidic environments.…”

Section: Magnetic Manipulation Techniquementioning

confidence: 99%

Single Cell Manipulation Technology

Lin¹,

Chen²,

Xie³

et al. 2015

Nano BioMed ENG

View full text Add to dashboard Cite

With microfluidic technique emerging, cell manipulation technology combines with microfluidic become a promising tool for single-cell-level manipulation. To obtain a kind of or single pure target cell for eliminating interference of useless cells in the trial of molecular biology, genetic analysis, proteomics and single cell analysis, various cell manipulation techniques have been developed for recovery specific cells. In this review, we introduce the principles of each cell manipulation technology and overlook the latest achievements of cell manipulation technique by categorizing externally applied manipulation forces: optical, electrical, magnetic, acoustic, mechanical. We also summarize the advantages and drawbacks of each cell manipulation technique.

show abstract

Magnetic tweezers for manipulation of magnetic particles in single cells

Cited by 22 publications

References 23 publications

Remote Control of T Cell Activation Using Magnetic Janus Particles

Remote Control of T Cell Activation Using Magnetic Janus Particles

Remote Control of T Cell Activation Using Magnetic Janus Particles

Single Cell Manipulation Technology

Contact Info

Product

Resources

About