Magnoflorine from Tinospora cordifolia stem inhibits α-glucosidase and is antiglycemic in rats

Abstract

A B S T R A C TAntidiabetic potential of Tinospora cordifolia stem is well proven. In the course of screening of useful a-glucosidase inhibitors, we prepared alkaloid fraction (AFTC) and isolated three isoquinoline alkaloids, namely, jatrorrhizine, palmatine and magnoflorine as active candidates for a-glucosidase inhibition. The enzyme kinetics was studied using sucrose and maltose as substrates. Michaelis-Menten constant (K m ) and maximal velocity (V max ) values were estimated. A significant decrease in V max and unaltered K m was observed in case of jatrorrhizine and palmatine (non-competitive inhibition). Magnoflorine was found to increase apparent K m and shown to be reversible, competitive inhibition. The IC 50 value as sucrase inhibitor was 36.25, 23.46 and 9.8 lg/mL for jatrorrhizine, palmatine and magnoflorine, respectively, and as maltase inhibitor was 22.05, 38.42 and 7.6 lg/mL for jatrorrhizine, palmatine and magnoflorine, respectively. In vivo studies were conducted on rats to determine oral glucose tolerance test (OGTT), using different substrates: glucose, sucrose and maltose. The increase in plasma glucose level was significantly suppressed (P < 0.01) by all the three alkaloids at 20 mg/kg b.w. Magnoflorine possessed the most potential activity as a-glucosidase inhibitor in vitro and in vivo.

Potential Biological Activities of Magnoflorine: A Compound from Aristolochia debilis Sieb. et Zucc

“…Hung et al (2007) also have reported that, magnoflorine played a role in protecting high-density lipoprotein (HDL) under oxidative stress. Different to the report by Patel and Mishra (2012), which indicated that the magnoflorine from Tinospora cordifolia stem inhibited α -glucosidase ability, the α-glucosidase inhibitory in this study was weak by a in vitro test (data not shown).…”

“…The antioxidant activity of different structural feature and physicochemical properties of the magnoflorine have been test in inhibit free radical, peroxidation of dioleoyl phosphatidylcholine, and liposomes (Hung et al, 2007). Magnoflorine also has been reported to have the effect on inhibiting α-glucosidase in vitro and in vivo (Patel and Mishra, 2012).…”

Kapasitas Antioksidan Dan Inhibitor Alfa Glukosidase Ekstrak Umbi Bawang Dayak

“…Banyak penelitian telah membuktikan bahwa senyawa fitokimia memiliki kemampuan untuk menghambat kerja enzim alfa glukosidase, seperti senyawa dari golongan alkaloid (Patel et al 2012), triterpenes (Lai et al 2012), dan flavonoid (Wang et al 2010 a ) Penghambatan aktivitas alfa glukosidase oleh berbagai senyawa fenolik juga telah banyak dijelaskan dalam literatur, dimana antara lain disebutkan bahwa alfa glukosidase secara efektif dihambat oleh flavonol (Lee et al 2008), luteolin, myricetin, dan quercetin (Tadera et al 2006). Dengan demikian kemampuan aktivitas inhibitor alfa glukosidase yang dimiliki oleh ekstrak umbi bawang dayak tidak lepas dari kerja senyawa fitokimia yang dikandungnya.…”

Investigation of the Safety and Antihyperglycemic Effect of <i>Apios americana</i> Flower Intake as a Food Material in Normal and Diabetic Mice

“…To date, a number of natural compounds that act as α-glucosidase inhibitors have been found in food (Peyrieras et al, 1983;Patel and Mishra, 2012), and many researches have elucidated the preventive effects of food ingredients on hyperglycemia (Shipra et al, 2010;Oe and Ozaki, 2008). Therefore, the identification of natural α-glucosidase inhibitors in food materials is of value in the development of new functional foods for the prevention of diabetes development and to reduce its incidence.…”