Major histocompatibility complex class IIA and IIB genes of loach (Misgurnus anguillicaudatus): Molecular cloning and expression analysis in response to bacterial and parasitic challenge

Huang, Zhenyu; Yu, Yongyao; Dong, Shuai; Yin, Yaxing; Wang, Qingchao; Yu, Wei; Luo, Yanzhi; Xu, Jie; Zhang, Liqiang; Yu, Yunzhen; Ai, Taoshan; Xu, Zhen

doi:10.1016/j.aquaculture.2018.10.043

Cited by 10 publications

(11 citation statements)

References 49 publications

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“…To further analyse the function of MHC II in pufferfish, the expressions of Pf-MHC IIα and Pf-MHC IIβ in skin, gills, gut and kidney were detected after A. hydrophila challenge in vivo. The expressions of Pf-MHC IIα and Pf-MHC IIβ were up-regulated in these four tissues under A. hydrophila challenge, which were similar to the results in loach (Huang et al, 2019), orange-spotted groupers (Epinephelus coioides) (Deng et al, 2020),…”

Section: Discussionsupporting

confidence: 82%

Major histocompatibility complex class IIα and IIβ of pufferfish (Takifugu obscurus): Identification and functional characterization in response to bacterial challenge

Qian

Wang

et al. 2023

Journal of Fish Diseases

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Classical major histocompatibility complex (MHC) class II molecules play an essential role in immune system. In this study, MHC IIα (Pf‐MHC IIα) and MHC IIβ (Pf‐MHC IIβ) homology genes from pufferfish (Takifugu obscurus) were cloned and their functional characterization in response to bacterial challenge was identified. The nucleotide sequences of the open reading frames (ORFs) of pufferfish Pf‐MHC IIα and Pf‐MHC IIβ were 708 bp and 750 bp, encoding 235 aa and 249 aa, respectively. The structure of Pf‐MHC IIα or Pf‐MHC IIβ contained a signal peptide, an α1/β1 domain, an α2/β2 domain, a transmembrane region and a cytoplasmic region. Multiple sequence alignment and phylogenetic analysis showed that Pf‐MHC IIα and Pf‐MHC IIβ molecules had the highest similarity with Fugu rubripes (Takifugu rubripes). Cellular localization analysis indicated that the distribution of Pf‐MHC IIα and Pf‐MHC IIβ was in the lymphocyte membrane and cytoplasm. qRT‐PCR results showed that Pf‐MHC IIα and Pf‐MHC IIβ expressed relatively high in skin, gills and gut. In addition, after stimulation challenge in vitro (lipopolysaccharide, or polyinosinic: polycytidylic acid) and in vivo (A. hydrophila), the mRNA expressions of Pf‐MHC IIα and Pf‐MHC IIβ were significantly up‐regulated in lymphocytes and in tissues of skin, gills, gut and head kidney. Moreover, Pf‐MHC IIα or Pf‐MHC IIβ neutralization reduced the ability of A. hydrophila to induce the expressions of lymphocyte cytokines (TNF‐α, IL‐1β and IL‐10). Overall, it is speculated that Pf‐MHC IIα and Pf‐MHC IIβ may play an important role in the host response against A. hydrophila in pufferfish.

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Section: Discussionsupporting

confidence: 82%

Major histocompatibility complex class IIα and IIβ of pufferfish (Takifugu obscurus): Identification and functional characterization in response to bacterial challenge

Qian

Wang

et al. 2023

Journal of Fish Diseases

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“…mhc 2 genes are vital members of the MHC family and play a key role in binding foreign peptides resulting from extracellular pathogens and releasing them to support T cells 66 . Even though there are many studies of mhc genes in teleost 25 , 67 , materials are still inadequate that teleost mhc genes play a part in immune defence thru infection with diverse pathogens. In the present study, the expression patterns of mhc 1 and mhc 2 genes in the midguts of cobia have been investigated after 10 weeks of autochthonous strains supplementation.…”

Section: Discussionmentioning

confidence: 99%

“…The up-regulation in the intestines indicates that the fish immune system could respond when pathogenic antigens move in Refs. 25 , 67 . Earlier, researchers have reported that mhc genes are accountable for stimulating specific immune reactions in the direction of the foreign pathogens 68 , 69 .…”

Section: Discussionmentioning

confidence: 99%

“…In distinction, only on dendritic cells , B cells, and macrophages that those of MHC II molecules can be found and release abnormal pathogen antigens for the preliminary stimulation of T cells 24 . In vertebrates comprising teleost fishes, the major histocompatibility complex (MHC) is an essential component of the immune response and is accountable for the foreign antigens presentation 25 .…”

Section: Introductionmentioning

confidence: 99%

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Effects of autochthonous strains mixture on gut microbiota and metabolic profile in cobia (Rachycentron canadum)

Amenyogbe

Luo

et al. 2022

Sci Rep

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The fish immune system is a topic or subject that offers a unique understanding of defensive system evolution in vertebrate heredity. While gut microbiota plays several roles in fish: well-being, promoting health and growth, resistance to bacterial invasion, regulation of energy absorption, and lipid metabolism. However, studies on fish gut microbiota face practical challenges due to the large number of fish varieties, fluctuating environmental conditions, and differences in feeding habits. This study was carried out to evaluate the impacts of supplemented three autochthonous strains, Bacillus sp. RCS1, Pantoeaagglomerans RCS2, and Bacilluscereus RCS3 mixture diet on cobia fish (Rachycentroncanadum). Also, chromatography, mass spectrometry and high throughput sequencing were combined to explore composition and metabolite profile of gut microbiota in juvenile cobia fed with supplemented diet. In the trial group, juvenile cobia received diets supplemented with 1 × 1012 CFU mL−1 autochthonous strains for ten weeks and a control diet without supplementation. Juvenile cobia receiving diets supplementation exhibited significantly improved growth than those without additives (control). Haematological indices, such as red blood cells, white blood cells, corpuscular haemoglobin concentration, mean corpuscular volume, haemoglobin, and mean corpuscular haemoglobin, were higher in the supplemented group. Similarly, digestive enzymes (trypsin, lipase, amylase, pepsin and cellulose, activities) activities were higher in supplemented diet with an indigenous isolates mixture. Serum biochemical parameters albumin, globulin, and total protein were significantly higher, while triglyceride, alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, and cholesterol showed no significant difference. On the other hand, glucose was significantly (P < 0.05) higher in the group without supplementation. On gene expression in the midgut, Immunoglobulin, Colony-stimulating factor receptor 1, major histocompatibility complex 1 were up-regulated by native isolates while T cell receptor beta, and Major histocompatibility complex 2 showed no significant difference. Gut bacterial composition was altered in fish receiving supplemented diet with autochthonous strains. Metabolomics also revealed that some metabolic pathways were considerably enriched in fish fed with supplemented diet; pathway analysis based on Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment revealed that differentially expressed metabolites were involved in galactose metabolism, tryptophan metabolism, carbohydrate digestion and absorption, purine metabolism, and ABC transporters. Functional analysis of bacterial community showed that differences in enriched metabolic pathways generally comprised carbohydrate and its metabolites, nucleotide and its metabolites, amino acid and its metabolites, heterocyclic compounds, and tryptamines, cholines, pigments. The current investigation results showed that autochthonous strains mixture has significantly enhanced the growth, survival, and innate and adaptive immunities of juvenile cobia.

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“…In the past, the immune system in Osteichthyes has been perceived as a system that is functionally limited to innate immune mechanisms, although research into MHC molecule genes in these animals was undertaken as long ago as in the 1970s and covered, among other fish species, zebrafish (Danio rerio, synonym Brachydanio rerio), rainbow trout (Oncorhynchus mykiss), olive flounder (Paralichthys olivaceus), European bass (Dicentrarchus labrax), channel catfish (Ictalurus punctatus), and turbot (Scophthalmus maximus) (17). Based on analysis of available genome sequences, five gene lineages of MHC class I molecules (MHC I-U, -Z, -S, -L, and -P) and one gene lineage of MHC class II molecules (MHC II-D) have been identified in Osteichthyes.…”

Section: Introductionmentioning

confidence: 99%

Major histocompatibility complex in Osteichthyes

Stosik

Tokarz–Deptuła

Deptuła

2020

Journal of Veterinary Research

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Based on analysis of available genome sequences, five gene lineages of MHC class I molecules (MHC I-U, -Z, -S, -L and -P) and one gene lineage of MHC class II molecules (MHC II-D) have been identified in Osteichthyes. In the latter lineage, three MHC II molecule sublineages have been identified (MHC II-A, -B and -E). As regards MHC class I molecules in Osteichthyes, it is important to take note of the fact that the lineages U and Z in MHC I genes have been identified in almost all fish species examined so far. Phylogenetic studies into MHC II molecule genes of sublineages A and B suggest that they may be descended from the genes of the sublineage named A/B that have been identified in spotted gar (Lepisosteus oculatus). The sublineage E genes of MHC II molecules, which represent the group of non-polymorphic genes with poor expression in the tissues connected with the immune system, are present in primitive fish, i.e. in paddlefish, sturgeons and spotted gar (Lepisosteus oculatus), as well as in cyprinids (Cyprinidae), Atlantic salmon (Salmo salar), and rainbow trout (Oncorhynchus mykiss). Full elucidation of the details relating to the organisation and functioning of the particular components of the major histocompatibility complex in Osteichthyes can advance the understanding of the evolution of the MHC molecule genes and the immune mechanism.

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Major histocompatibility complex class IIA and IIB genes of loach (Misgurnus anguillicaudatus): Molecular cloning and expression analysis in response to bacterial and parasitic challenge

Cited by 10 publications

References 49 publications

Major histocompatibility complex class IIα and IIβ of pufferfish (Takifugu obscurus): Identification and functional characterization in response to bacterial challenge

Major histocompatibility complex class IIα and IIβ of pufferfish (Takifugu obscurus): Identification and functional characterization in response to bacterial challenge

Effects of autochthonous strains mixture on gut microbiota and metabolic profile in cobia (Rachycentron canadum)

Major histocompatibility complex in Osteichthyes

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