MALDI‐TOF Mass Spectrometry of Intact Bacteria

Lay, Jackson O.; Liyanage, Rohana

doi:10.1002/0471748641.ch6

Cited by 6 publications

(3 citation statements)

References 38 publications

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Section: Introductionmentioning

confidence: 99%

“…However, methods for the identification of aerosol samples containing spores are limited. Furthermore, current methods are largely dependent on various extraction methods (Fenselau and Demirev 2001;Lay and Liyanage 2006;Barbuddhe et al 2008;Moura et al 2008;Lasch et al 2009;He et al 2011;Welker and Moore 2011;Drevinek et al 2012;Goldstein et al 2013). Our research team recently developed a direct in situ analytical method that overcomes these disadvantages.…”

Section: Introductionmentioning

confidence: 99%

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Rapid detection of Bacillus spore aerosol particles by direct in situ analysis using MALDI-TOF mass spectrometry

Jeong

Choi

Chong

et al. 2014

Lett Appl Microbiol

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Significance and Impact of the Study: Methods that permit accurate and fast detection and identification are essential in the case of pathogens such as Bacillus anthracis, which can be used as biological warfare agents or bioterrorism materials. Here, direct in situ MALDI-TOF MS allowed the high-throughput detection and identification of aerosolized Bacillus spore particles, and this method could be effectively applied for the detection and inspection of BWAs. In this context, we believe that this method may contribute to the realization of real-time and on-site identification of BWAs. AbstractAccurate and rapid analytical methods are essential for the detection and identification of biological warfare agents (BWA). Although various studies have investigated the use of MALDI-TOF MS for bacterial classification, only a few studies have examined the applicability of method for the identification of BWAs. This study aimed to generate, collect and analyse Bacillus spore aerosol particles of 2-10 lm, the optimal size of a BWA. Bacillus spore aerosol particles of 2-10 lm were rapidly analysed using direct in situ MALDI-TOF MS without pretreatment processes. A mass spectrum of Bacillus spore aerosol particles was successfully detected. For real-time detection and identification, a mass spectral database of Bacillus spores was constructed, and an algorithm was developed and applied. Bacillus spore particles were rapidly detected and identified by MS, which can be used for the detection and inspection of BWAs.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Rapid detection of Bacillus spore aerosol particles by direct in situ analysis using MALDI-TOF mass spectrometry

Jeong

Choi

Chong

et al. 2014

Lett Appl Microbiol

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“…A matrix-assisted laser desorption/ionization-time-offlight mass spectrometry (MALDI-TOF MS) has been introduced in bacterial taxonomy and successfully applied to many taxa in a high-throughput manner. [5][6][7][8][9][10][11] In some cases microorganism identification by MALDI-TOF MS, direct whole cell mass spectrometry was applied. This method was simple and could be successfully used for analyzing vegetative cells or colonies.…”

mentioning

confidence: 99%

Discrimination of Bacillus anthracis Spores by Direct in-situ Analysis of Matrix-Assisted Laser Desorption/Ionization Time-Of-Flight Mass Spectrometry

Jeong¹,

Lee²,

Kim³

2013

Bulletin of the Korean Chemical Society

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The rapid and accurate identification of biological agents is a critical step in the case of bio-terror and biological warfare attacks. Recently, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry has been widely used for the identification of microorganisms. In this study, we describe a method for the rapid and accurate discrimination of Bacillus anthracis spores using MALDI-TOF MS. Our direct in-situ analysis of MALDI-TOF MS does not involve subsequent high-resolution mass analyses and sample preparation steps. This method allowed the detection of species-specific biomarkers from each Bacillus spores. Especially, B. anthracis spores had specific biomarker peaks at 2503, 3089, 3376, 6684, 6698, 6753, and 6840 m/z. Cluster and PCA analyses of the mass spectra of Bacillus spores revealed distinctively separated clusters and withingroups similarity. Therefore, we believe that this method is effective in the real-time identification of biological warfare agents such as B. anthracis as well as other microorganisms in the field.

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