Maleimide Cross‐Linked Bioactive PEG Hydrogel Exhibits Improved Reaction Kinetics and Cross‐Linking for Cell Encapsulation and In Situ Delivery

Phelps, Edward A.; Enemchukwu, Nduka; Fiore, Vincent F.; Sy, Jay C.; Murthy, Niren; Sulchek, Todd; Barker, Thomas H.; Garcı́a, Andrés J.

doi:10.1002/adma.201103574

Cited by 491 publications

(532 citation statements)

References 46 publications

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“…The chemistry of PEG-MAL hydrogels allows for gelation at neutral pH, 27 and the encapsulated cells were not exposed to UV light, as is common in other systems. 9,11 The PEG-based hydrogel system allows the specific incorporation of cell-binding and cell-degradable functional groups, which is not possible with protein-based gels such as collagen 51 or Matrigel.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Complementary, Semiautomated Methods for Creating Multidimensional PEG-Based Biomaterials

Brooks

Jansen

Gencoglu

et al. 2018

ACS Biomater. Sci. Eng.

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Tunable biomaterials that mimic selected features of the extracellular matrix (ECM), such as its stiffness, protein composition, and dimensionality, are increasingly popular for studying how cells sense and respond to ECM cues. In the field, there exists a significant trade-off for how complex and how well these biomaterials represent the in vivo microenvironment, versus how easy they are to make and how adaptable they are to automated fabrication techniques. To address this need to integrate more complex biomaterials design with high-throughput screening approaches, we present several methods to fabricate synthetic biomaterials in 96-well plates and demonstrate that they can be adapted to semiautomated liquid handling robotics. These platforms include 1) glass bottom plates with covalently attached ECM proteins, and 2) hydrogels with tunable stiffness and protein composition with either cells seeded on the surface, or 3) laden within the three-dimensional hydrogel matrix. This study includes proof-of-concept results demonstrating control over breast cancer cell line phenotypes via these ECM cues in a semi-automated fashion. We foresee the use of these methods as a mechanism to bridge the gap between high-throughput cell-matrix screening and engineered ECM-mimicking biomaterials.

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Section: Discussionmentioning

confidence: 99%

“…Sigma-Aldrich) dissolved in 2 mM triethanolamine (pH 7.4) as a catalyst. 27 Final hydrogel volume was 10 L, which covers the surface of the well once swollen. After waiting five minutes to ensure hydrogels were completely polymerized, the hydrogels were swollen overnight by adding 100 L/well PBS.…”

Section: Basic Liquid Handling Robotics Operationmentioning

confidence: 99%

Complementary, Semiautomated Methods for Creating Multidimensional PEG-Based Biomaterials

Brooks

Jansen

Gencoglu

et al. 2018

ACS Biomater. Sci. Eng.

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“…These step-growth polymerizations, which utilize multi-arm PEG star polymers synthesized by living polymerization methods, are also believed to produce more uniform polymer networks. Typically, Michael-type thiol-ene crosslinking is performed using PEG macromers functionalized with vinyl sulfone [28] or maleimide [29] groups, since the acrylate-thiol reaction produces esters with neighboring sulfides that are readily hydrolyzed under physiologic conditions. [30] The thiol-ene photoclick reaction is performed using norbornene-functionalized PEG.…”

Section: Overview Of Tissue-mimetic Hydrogelsmentioning

confidence: 99%

Shooting for the moon: using tissue-mimetic hydrogels to gain new insight on cancer biology and screen therapeutics

2017

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Tissue engineering holds great promise for advancing cancer research and achieving the goals of the Cancer Moonshot by providing better models for basic research and testing novel therapeutics. This paper focuses on the use of hydrogel biomaterials due to their unique ability to entrap cells in three-dimensional (3D) matrix that mimics tissues and can be programmed with physical and chemical cues to recreate key aspects of tumor microenvironments. The chemistry of some commonly used hydrogel platforms is discussed, and important examples of their use in tissue engineering 3D cancer models are highlighted. Challenges and opportunities for future research are also discussed.

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“…Another important fact is that the swelling process is reversible. Hydrogels based on both natural and synthetic polymers still are of interest for cell encapsulation applications [14][15][16][17]. Recently, such hydrogels have become especially attractive for the field of tissue engineering as matrices for repairing and regenerating a wide variety of tissues and organs [18][19][20][21]; also these materials are actually used for the fabrication of drug delivery systems, separation operations in biotechnology, processing of agricultural products, conductive and superabsorbent composites, sensors and actuators [22][23][24].…”

Section: Introductionmentioning

confidence: 99%

In situ silver nanoparticle formation embedded into a photopolymerized hydrogel with biocide properties

et al. 2014

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In situ nanoparticle formation embedded into hydrogel matrix, acting as container and stabilizer for nanoparticle reaction was the focus in this research; this method was realized using AgNO 3 (0.75 and 1.0 M) as silver source for nanoparticle formation; also, monomers (HEMA), cross-linker agents (DEGDMA) and a photoinitiator (Irgacure 651) were used for the hydrogel synthesis. For the reduction of Ag ? ? Ag 0 , the reaction mixture was irradiated with an UV lamp at 365 nm for 30 min; parallel to this process, the hydrogel photopolymerization occurs. All these systems were studied by Infrared and Raman Spectroscopy, optical studies: UV/Vis absorption, thermal studies: differential scanning calorimetry and thermogravimetric analysis, X-ray diffraction, fluorescence X-ray spectroscopy and transmission electronic microscopy. Characterization techniques are capable to detect the presence of non-agglomerated silver nanoparticles homogeneously distributed in all the systems. X-Ray photoemission spectroscopy establishes the presence of Ag 0 and Ag ? as mixture in the synthesized composite. Quantitative assays show that the sample Ag_Hg3-89.5 % (1.0 M) presents an important biocide property, by reducing 99.9 % of bacterium Escherichia coli ATCC 25922 as compared with the alone hydrogel used as control.

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Maleimide Cross‐Linked Bioactive PEG Hydrogel Exhibits Improved Reaction Kinetics and Cross‐Linking for Cell Encapsulation and In Situ Delivery

Cited by 491 publications

References 46 publications

Complementary, Semiautomated Methods for Creating Multidimensional PEG-Based Biomaterials

Complementary, Semiautomated Methods for Creating Multidimensional PEG-Based Biomaterials

Shooting for the moon: using tissue-mimetic hydrogels to gain new insight on cancer biology and screen therapeutics

In situ silver nanoparticle formation embedded into a photopolymerized hydrogel with biocide properties

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