Malonic Acid Isolated from Pinus densiflora Inhibits UVB-Induced Oxidative Stress and Inflammation in HaCaT Keratinocytes

Park, Cheolwoo; Park, Jaeyoung; Kim, Won-Jin; Kim, Woong; Cheong, Hyeonsook; Kim, Seok-Jun

doi:10.3390/polym13050816

Cited by 17 publications

(13 citation statements)

References 41 publications

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“…Regulation of Nrf2 activity has also been proposed to offer a potential strategy to improve skin barrier integrity by mitigating UVR-induced damage of keratinocytes and modulating inflammatory responses of the skin. Downregulation of Nrf2 signaling was shown to be involved in UVB-induced upregulation of pro-inflammatory mediators such as tumor necrosis factor alpha (TNF-α), cyclooxygenase-2 (COX-2), interleukin-6 (IL-6), interleukin-1 beta (IL-1β), and interleukin-8 (IL-8) in keratinocyte HaCaT cells ( Park et al, 2021 ). A previous study using a mouse model of UVB-induced photodamage revealed that basal activity of Nrf2 in keratinocytes of normal skin is vital for improvement of skin barrier integrity and for prevention of skin carcinogenesis.…”

Section: Introductionmentioning

confidence: 99%

Role of Phytochemicals in Skin Photoprotection via Regulation of Nrf2

Chaiprasongsuk

Panich

2022

Front. Pharmacol.

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Ethnopharmacological studies have become increasingly valuable in the development of botanical products and their bioactive phytochemicals as novel and effective preventive and therapeutic strategies for various diseases including skin photoaging and photodamage-related skin problems including abnormal pigmentation and inflammation. Exploring the roles of phytochemicals in mitigating ultraviolet radiation (UVR)-induced skin damage is thus of importance to offer insights into medicinal and ethnopharmacological potential for development of novel and effective photoprotective agents. UVR plays a role in the skin premature aging (or photoaging) or impaired skin integrity and function through triggering various biological responses of skin cells including apoptosis, oxidative stress, DNA damage and inflammation. In addition, melanin produced by epidermal melanocytes play a protective role against UVR-induced skin damage and therefore hyperpigmentation mediated by UV irradiation could reflect a sign of defensive response of the skin to stress. However, alteration in melanin synthesis may be implicated in skin damage, particularly in individuals with fair skin. Oxidative stress induced by UVR contributes to the process of skin aging and inflammation through the activation of related signaling pathways such as the mitogen-activated protein kinase (MAPK)/activator protein-1 (AP-1), the phosphatidylinositol 3-kinase (PI3K)/protein kinase B (Akt), the nuclear factor kappa B (NF-κB) and the signal transducer and activator of transcription (STAT) in epidermal keratinocytes and dermal fibroblasts. ROS formation induced by UVR also plays a role in regulation of melanogenesis in melanocytes via modulating MAPK, PI3K/Akt and the melanocortin 1 receptor (MC1R)-microphthalmia-associated transcription factor (MITF) signaling cascades. Additionally, nuclear factor erythroid 2-related factor 2 (Nrf2)-regulated antioxidant defenses can affect the major signaling pathways involved in regulation of photoaging, inflammation associated with skin barrier dysfunction and melanogenesis. This review thus highlights the roles of phytochemicals potentially acting as Nrf2 inducers in improving photoaging, inflammation and hyperpigmentation via regulation of cellular homeostasis involved in skin integrity and function. Taken together, understanding the role of phytochemicals targeting Nrf2 in photoprotection could provide an insight into potential development of natural products as a promising strategy to delay skin photoaging and improve skin conditions.

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Section: Introductionmentioning

confidence: 99%

Role of Phytochemicals in Skin Photoprotection via Regulation of Nrf2

Chaiprasongsuk

Panich

2022

Front. Pharmacol.

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“…Accumulated cellular ROS significantly stimulates skin inflammation. According to previous studies, PCS extract attenuates free radicals and the overproduction of ROS in HaCaT cells induced by UVB [17], and we hypothesized that PCS extract might prevent UVB-induced inflammatory response in keratinocyte cells, as it is well known that ROS directly related to NF-κB activation is a key mediator of inflammation regulating gene expression, including COX-2, iNOS, TNF-α, IL-6, IL-8, and IL-1β [8,30,31]. In keratinocyte cells, NF-κB increased the sensitivity of inflammatory response in UVB-irradiated cells and promoted inflammatory cytokine expression [7,16,[32][33][34].…”

Section: Discussionmentioning

confidence: 84%

“…This event consequently improved the activities of the transcriptional factor subunit of the activator protein 1 (AP-1) complex. The increased AP-1 activity up-regulated proinflammatory cytokines, including COX2 and iNOS [8,11,30,37]. Moreover, Spiegelman et al (2000) suggested that activation of JNK resulted in the accumulation of β-transducin repeat-containing protein (β-TrCP), which mediated the ubiquitination of phosphorylated IκBα protein.…”

Section: Discussionmentioning

confidence: 99%

Purple Corn Silk Extract Attenuates UVB-Induced Inflammation in Human Keratinocyte Cells

et al. 2022

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UVB is a causative factor for severe skin damage, such as cell aging, death, and inflammation. UVB easily permeates into the epidermis layer of human skin, which is mainly composed of keratinocyte cells. In previous results, we found that purple corn silk (PCS) extract showed the potential to inhibit keratinocyte damages of UVB-treated cells. Thus, in this study, we aimed to evaluate the preventive effects of PCS extract against the inflammation of UVB-induced keratinocyte cells using the HaCaT cell line. HaCaT cells were treated with PCS extract at various concentrations for 1 h, then exposed to 25 mJ/cm2 UVB before subsequent experiments. Fragmented DNA was observed using flow cytometry. The inflammatory response was investigated through NF-κB activity by immunofluorescence staining and related protein expression by Western blotting. The results demonstrated that PCS extract decreased the sub-G1 DNA content. Interestingly, PCS extract attenuated NF-κB activity via suppressed NF-κB nuclear translocation and protein expression. Moreover, PCS extract remarkably decreased c-Jun phosphorylation and decreased proinflammatory cytokines, along with iNOS and COX-2 levels in UVB-treated cells compared to the UVB-control group. This finding exhibited that PCS extract minimized inflammation in keratinocyte cells induced by UVB radiation.

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“…Nrf2 can establish a glutathione-mediated UVB detoxification mechanism in the epidermis, alleviate the toxic effects of UVB on epidermal cells and inhibit the apoptosis of mouse epidermal cells [ 40 ]. Malonic acid extracted from Korean pine can increase the levels of antioxidant enzymes HO-1 and SOD by regulating the Nrf2 signaling pathway, and increase the survival rate of HaCaT cells by inhibiting ROS, confirming that it can inhibit skin inflammation [ 41 ]. In addition, Nrf2 affects the downstream AP-1 gene, which in turn affects the expression of genes such as IL-1β, TNF-α and MMPs, and regulates the body’s inflammatory response process [ 27 ].…”

Section: Discussionmentioning

confidence: 99%

Two Laminaria japonica Fermentation Broths Alleviate Oxidative Stress and Inflammatory Response Caused by UVB Damage: Photoprotective and Reparative Effects

et al. 2022

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UVB radiation can induce oxidative stress and inflammatory response in human epidermal cells. We establish a UVB-induced damage model of human immortalized epidermal keratinocytes (HaCaT) to explore the protective and reparative effects of Laminaria japonica on UVB-damaged epidermal inflammation after fermentation by white Ganoderma lucidum (Curtis) P. Karst and Saccharomyces cerevisiae. Compared with unfermented Laminaria japonica, fermented Laminaria japonica possesses stronger in vitro free radical scavenging ability. Laminaria japonica white Ganoderma lucidum fermentation broth (LJ-G) and Laminaria japonica rice wine yeast fermentation broth (LJ-Y) can more effectively remove excess reactive oxygen species (ROS) in cells and increase the content of the intracellular antioxidant enzymes heme oxygenase-1 (HO-1) and NAD(P)H quinone oxidoreductase 1 (NQO-1). In addition, fermented Laminaria japonica effectively reduces the content of pro-inflammatory factors ILs, TNF-α and MMP-9 secreted by cells. The molecular research results show that fermented Laminaria japonica activates the Nrf2 signaling pathway, increases the synthesis of antioxidant enzymes, inhibits the gene expression levels of pro-inflammatory factors, and alleviates cellular oxidative stress and inflammatory response caused by UVB radiation. Based on the above results, we conclude that fermented Laminaria japonica has stronger antioxidant and anti-inflammatory activity than unfermented Laminaria japonica, possesses good safety, and can be developed and used as a functional inflammation reliever. Fermented Laminaria japonica polysaccharide has a more slender morphological structure and more rockulose, with better moisturizing and rheological properties.

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Malonic Acid Isolated from Pinus densiflora Inhibits UVB-Induced Oxidative Stress and Inflammation in HaCaT Keratinocytes

Cited by 17 publications

References 41 publications

Role of Phytochemicals in Skin Photoprotection via Regulation of Nrf2

Role of Phytochemicals in Skin Photoprotection via Regulation of Nrf2

Purple Corn Silk Extract Attenuates UVB-Induced Inflammation in Human Keratinocyte Cells

Two Laminaria japonica Fermentation Broths Alleviate Oxidative Stress and Inflammatory Response Caused by UVB Damage: Photoprotective and Reparative Effects

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