Management of calves in commercial dairy farms in Mecklenburg-Western Pomerania, Germany and its impact on calf mortality and prevalence of rotavirus and Cryptosporidium parvum infections in pre-weaned calves

Falkenberg, U.; Krömker, Volker; Konow, M.; Flor, J.; Sanftleben, P.; Losand, B.

doi:10.1016/j.vas.2022.100243

Cited by 5 publications

(2 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Здоров'я телят і благополуччя тварин становлять інтерес для виробників, споживачів і лікарів (Falkenberg et al, 2022). Високі показники захворюваності та смертності призводять до величезних економічних втрат, суперечать добробуту тварин і безпечності харчових продуктів.…”

Section: вступunclassified

Development of methods for prevention of cryptosporidiosis of calves

Фотіна¹,

Шкромада²,

Berezovsky³

et al. 2022

SMLNUVMB

View full text Add to dashboard Cite

Cryptosporidiosis of calves, caused by the parasite Cryptosporidium parvum, is a dangerous disease for calves for up to one month. Sick animals suffer from debilitating diarrhea, intoxication, and dehydration, which can lead to death. Currently, effective preventive and curative measures have not been developed. Therefore, new strategies for preventing cryptosporidiosis in cattle are being sought. The study was conducted on the farm for raising young cattle in Holstein LTD agricultural firm “Lan” Sumy region in February 2021. The circulating microflora in the calf and the clinical condition of the animals were determined. The first experimental group of calves used the antimicrobial agent Trimeratinvet powder (water-soluble powder for oral administration). In the second experimental group, calves were fed the acidifier Kronocid L. As a control, healthy calves were used, which received a regular diet. Conducted a study of fecal masses of calves from birth to one month. The presence of Cryptosporidium spp. in fecal masses of calves was determined microscopically and by nested polymerase chain reaction. The count of microorganisms was performed after cultivation on elective media and determined the number of colony-forming units in CFU/cm3. The EXPERTISTM Rainbow diagnostic kit was used to diagnose Rotavirus and Coronavirus. Studies have shown that Cryptosporidium in calves under one month of age is the most common cause of diarrhea (42 %). Acidifier Kronocid L and Trimeratinvet inhibited the reproduction of cryptosporidia by stopping sporulation. Acidifier at a pH of 4.5 penetrated the oocyst's shell, causing cytoplasm compression. At 60 minutes of exposure, Kronocid L reduced the possibility of sporulation and destroyed 90 % of treated oocysts, which is 20 % more than the antimicrobial agent Trimeratinvet. In the experimental group using Trimeratinvet, the onset of chewing occurred later than eight days compared to the control group and with Kronocid L. Calves of the experimental groups had an average weight gain during the month with Trimeratinvet less by 20.8 % (P ≤ 0.05), with Kronocid L – by 14.3 % compared to healthy. Prospects for further research are to determine the effect of Cronocid L and other species of Cryptosporidium in adult cattle.

show abstract

Section: вступunclassified

Development of methods for prevention of cryptosporidiosis of calves

Фотіна¹,

Шкромада²,

Berezovsky³

et al. 2022

SMLNUVMB

View full text Add to dashboard Cite

show abstract

“…The parasite is considered a significant cause of neonatal profuse watery diarrhea due to its life cycle of C. parvum is done in the epithelial cells in gut canal (3). Infection by C. parvum results in intercellular colonization of the extra-cytoplasmic microvillus of the small intestine (4) and leads to production losses and a high mortality rate in pre-weaning calves during the first 28 days of life (5). Cryptosporidium parvum is easily transmitted to humans, especially immune-compromised individuals, and is therefore considered an anthroponotic zoonotic disease (6(.…”

Section: Introductionmentioning

confidence: 99%

Detection of Cryptosporidium parvum in calf feces using microscopical, serological, and molecular methods

Felefel¹,

Abdel-Rady²,

El-Rahim³

et al. 2023

IJVS

View full text Add to dashboard Cite

Cryptosporidiosis is a critical gastrointestinal disease in calves. This study examined 150 fecal samples of diarrheic calves collected from the eastern region of Saudi Arabia for detection of Cryptosporidium parvum using the Modified Ziehl-Neelsen (MZN) method, Enzyme-Linked Immunosorbent Assay (ELISA), and conventional Polymerase Chain Reaction (PCR). The performance of these methods was assessed using diagnostic accuracy tests. The present study identified C. parvum oocysts in fecal samples by modified Ziehl-Neelsen 40/150; 26.66%, ELIZA 60/150; 40%, and PCR 78/150; 52% methods, respectively. The microscopic method revealed higher specificity 65.27% than the ELIZA 51.38%, while the ELIZA showed higher sensitivity 32.05% than MZN method 19.23%. However, MZN and ELIZA methods were unsatisfactory diagnostic tools compared with the PCR as the area under the curve values in Receiver Operator Characteristic (ROC) analysis were less than 0.6. Furthermore, using the kappa analysis test revealed no agreement between MZN and ELISA methods compared with PCR at P<0.05.

show abstract