Manganese deficiency alters arterial glycosaminoglycan structure in the Sprague-Dawley rat

Yang, Peiying; Klimis-Tavantzis, Dorothy J.

doi:10.1016/s0955-2863(98)00010-2

Cited by 3 publications

(8 citation statements)

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“…Previous studies have shown that Mn may affect blood pressure by decreasing the tension of isolated vascular tissue preparations in vitro [21]. However, there have been no animal studies to 0955 Our laboratory was the first to report that Mn plays an important role in maintaining integrity of blood vessels [22][23][24][25][26]. Manganese is involved in arterial glycosaminoglycan metabolism by affecting the total proteoglycan content of the aorta, altering the molecular weight and sulfation pattern of chondroitin sulfate in the same tissue and thus predisposing the vessel to lipid deposition, lipoprotein oxidation and CVD [24,25].…”

Section: Introductionmentioning

confidence: 99%

“…However, there have been no animal studies to 0955 Our laboratory was the first to report that Mn plays an important role in maintaining integrity of blood vessels [22][23][24][25][26]. Manganese is involved in arterial glycosaminoglycan metabolism by affecting the total proteoglycan content of the aorta, altering the molecular weight and sulfation pattern of chondroitin sulfate in the same tissue and thus predisposing the vessel to lipid deposition, lipoprotein oxidation and CVD [24,25]. Manganese is essential for preventing surface damage of the aorta and maintaining the density of the extracellular matrix around smooth muscle cells, especially in the medial layer [26].…”

Section: Introductionmentioning

confidence: 99%

“…Manganese is essential for preventing surface damage of the aorta and maintaining the density of the extracellular matrix around smooth muscle cells, especially in the medial layer [26]. Transmission electron microscopy of the arterial wall revealed less dense extracellular matrix around smooth muscle cells, especially in the medial layers of the Mn deficient rats, suggesting possible changes in the endothelial and/or vascular smooth muscle cells, which affect the contractile properties of the arteries [24,25].…”

Section: Introductionmentioning

confidence: 99%

“…In our study we examined the role of dietary Mn on the biomechanical properties and receptor sensitivity of Sprague-Dawley rat aortic rings, as assessed by aortic smooth muscle contraction in response to phenylephrine, an a 1 adrenergic agonist. [22][23][24][25]. Dietary Mn levels of 10 ppm have been reported by the American Institute of Nutrition as an adequate level to prevent deficiency in the rat [27].…”

Section: Introductionmentioning

confidence: 99%

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Dietary manganese suppresses α1 adrenergic receptor-mediated vascular contraction

Kalea

Harris

2005

The Journal of Nutritional Biochemistry

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Dietary manganese suppresses α1 adrenergic receptor-mediated vascular contraction

Kalea

Harris

2005

The Journal of Nutritional Biochemistry

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“…CRITCHFIELD ( A deficiência de manganês também foi associada à redução da biossíntese de glicosaminoglicanas (YANG, 1998), alteração da estrutura e movimento rotacional das proteínas da HDL 2 , com profundas alterações no metabolismo e transporte do colesterol em ratos (TAYLOR, 1997), além de redução do ganho de peso e anormalidades físicas (GONG et al, 1998). …”

Section: Deficiência De Manganêsunclassified

Preparação e caracterização físico-química de complexos de hidrolisados parciais de proteína com minerais

Franzan¹

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AGRADECIMENTOSPrimeiramente a Deus, pelos momentos de luz que sempre me guiaram ao alcance dos meus objetivos.Aos meus pais, Ivonete e Antonio, por acreditar e confiar sempre, pelo patrocínio dos meus sonhos, pelos ensinamentos ao longo de toda a vida, pela presença em todos os momentos que eu precisei e pelo amor incondicional e eterno que sempre teremos uns pelos outros.Ao meu irmão Ricardo, pelo carinho e estímulo de viver a vida intensamente.Ao meu companheiro Sérgio, por estar junto a mim em todos os momentos, bons ou ruins, sentindo sua presença e seu amor mesmo à distância. Pelo apoio, amor, carinho e principalmente pela compreensão. Por me fazer enxergar sempre o que há de bom ao meu redor com entusiasmo e alegria. Por me fazer feliz todos dias e ter dado à minha vida o amor, essencial, e o colorido que faltava para ela se tornar completa e plena.Ao meu orientador, Prof. Dr. Osvaldo de Freitas, por ter me ensinado que quando queremos muito alcançar um objetivo já percorremos metade de sua trajetória, por ter me dado a oportunidade e confiado em desenvolver esse trabalho, pelos ensinamentos e pela contribuição para o meu crescimento profissional, científico e intelectual. RESUMOOs minerais essenciais participam de várias funções vitais do organismo, suas deficiências contribuem de maneira significante para a morbidade e mortalidade na infância, especialmente nos países em desenvolvimento, sendo consideradas como problema de saúde pública em todo o mundo. Minerais apresentados na forma de complexos orgânicos têm sido propostos para correção destas deficiências por apresentarem maior biodisponibilidade, quando comparados aos equivalentes sais inorgânicos. Os minerais cobre, ferro, manganês e zinco são essenciais aos organismos vivos (humano e animal), a manutenção de seus níveis de acordo com as necessidade fisiológicas contribuem para a manutenção da saúde, resultando em benefícios econômicos. No presente trabalho foi realizada a preparação e caracterização físico-química de complexos dos minerais Cobre (Cu), Ferro (Fe), Manganês (Mn) e Zinco (Zn) com hidrolisados parciais de proteína de soja. As hidrólises utilizando as enzimas Aranzime e Alcalase foram mais eficientes que as demais hidrólises enzimáticas ou ácidas, entretanto somente a Alcalase foi aprovada para a manufatura de produtos alimentares de consumo humano. A coordenação entre os metais e o hidrolisado pode ser confirmada por pelo deslocamento das bandas ν NH2 , ν aCOO -e ν sCOO -. Os complexos obtidos de cobre, ferro, manganês e zinco, posuem em sua composição os respectivos percentuais de ligação: 2,21%, 1,22%, 12,38% e 10,40%. Abstract ABSTRACTThe essential minerals participate in many vital functions in the organism, its deficiencies contribute in significant way for the morbidity and mortality in the childhood, especially in developing countries, being considered as public health problem in the whole world. Minerals presented in the form of organics complexes have been considered a good choice for these deficiencies, because it presents g...

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Dietary manganese affects the concentration, composition and sulfation pattern of heparan sulfate glycosaminoglycans in Sprague-Dawley rat aorta

et al. 2006

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We examined the effect of dietary Mn on the composition and structure of heparan sulfate (HS) glycosaminoglycans (GAGs) of rat aorta. Animals were randomly assigned to either a Mn deficient (MnD), adequate (MnA) or supplemented (MnS) diet (Mn<1, 10-15 and 45-50 ppm, respectively). After 15 weeks, aortic tissue GAGs were isolated with papain digestion, alkaline borohydride treatment and anion-exchange chromatography. Cellulose acetate electrophoresis and treatment of the fractions with specific lyases revealed the presence of three GAG populations, i.e. hyaluronan (HA), heparan sulfate (HS) and galactosaminoglycans (GalAGs). Disaccharide composition of the HS fractions was determined by HPCE following treatment with heparin lyases I, II and III. In MnS aortas we observed increased concentration of total GalAGs and decreased concentration of HS and HA, when compared to MnA aortas. Aortas from MnD and MnA rats appeared to have similar distribution of individual GAGs. Heparan sulfate chains of MnS aortas contained higher (41%) concentration of non-sulfated units compared to MnA ones. Variable amounts of trisulfated and disulfated units were found only in MnD and MnA groups but not in MnS. Our results demonstrate that HS biosynthesis in the rat aorta undergoes marked structural modifications that depend upon dietary Mn intake. The reduced expression and undersulfation of HSPGs with Mn supplementation might indicate a reduced ability of vascular cells to interact with biologically active molecules such as growth factors. Alterations in cell-membrane binding ability to a variety of extracellular ligands might affect signal-transduction pathways and arterial functional properties.

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Manganese deficiency alters arterial glycosaminoglycan structure in the Sprague-Dawley rat

Cited by 3 publications

References 32 publications

Dietary manganese suppresses α1 adrenergic receptor-mediated vascular contraction

Dietary manganese suppresses α1 adrenergic receptor-mediated vascular contraction

Preparação e caracterização físico-química de complexos de hidrolisados parciais de proteína com minerais

Dietary manganese affects the concentration, composition and sulfation pattern of heparan sulfate glycosaminoglycans in Sprague-Dawley rat aorta

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