Mangiferin prevents the growth of gastric carcinoma by blocking the PI3K-Akt signalling pathway

Abstract

The aim of the present study was to investigate the effects of mangiferin on gastric carcinoma cells and to determine the possible mechanisms underlying such effects. The MTT assay was performed to evaluate the antiproliferative effect of mangiferin. Following treatment, apoptosis rates of SGC-7901 were established by flow cytometry and laser confocal microscopy, and western blot analysis was used to detect the expression of apoptosis-related proteins. The MTT assay showed that mangiferin inhibited the proliferation of SGC-7901 and BCG-823 cells in a dose-dependent and time-dependent manner. After SGC-7901 cells were exposed to mangiferin for 24, 48 and 72 h, the half-maximal inhibitory concentration values were 16.00, 8.63 and 4.79 µmol/l, respectively. SGC-7901 cell apoptosis induced by mangiferin was observed by Annexin V/PI doubling staining and terminal deoxynucleotidyl transferase dUTP nick end labeling-positive staining. We found a significant decrease in Bcl-2, Bcl-xL and Mcl-1 expression and a significant increase in Bax, Bad and cleaved caspase-3 and caspase-9 expression in SGC-7901 cells by mangiferin treatment. Moreover, mangiferin significantly decreased the levels of p-PI3K, p-Akt and p-mTOR, but had no effects on those of PI3K, Akt and mTOR in epidermal growth factor-treated SGC-7901 cells. Interestingly, the proapoptotic effect of mangiferin on SGC-7901 cells was partially blocked by the Akt activator SC79, whereas LY294002 significantly increased mangiferin-induced apoptosis and growth inhibition. Taken together, our findings indicate that mangiferin effectively inhibits cell growth and induces apoptosis of gastric cancer cells through inhibiting the PI3K/Akt pathways with relative safety, and may be used as a novel chemotherapeutic agent against gastric cancer.

Potential Therapeutic Value of Mangiferin for Lung Adenocarcinoma (Luad): A Comprehensive Study Based on in Vitro Experiments and Bioinformatics

“…Studies employing molecular biology and biochemical pharmacology to investigate the underlying mechanism governing these effects have opened new approaches for clinical antineoplastic drug development. In our study, mangiferin is a widely sourced treatment option that has shown therapeutic value in such cancer types as liver cancer, ovarian cancer, gastric cancer, and lung cancer [15,17,37,38], while potential targets, such as NFκB, PPARү, MMP-7, MMP-9 and EMT [14], were predicted. Similarly, we adopted the experimental method of a self-control study treated with mangiferin to explore the medicinal effect of mangiferin in LUAD cell lines.…”

Mangiferin induces immune responses and evaluates the survival rate in WEHI‐3 cell generated mouse leukemia in vivo

“…Mangiferin presents various pharmacological activity, including anti-cancer activity in many human cancer cell lines. [12][13][14][16][17][18][19][20][21][22][23][24]28 In the present study, we used murine acute myelomonocytic leukemia WEHI-3 cells to generate AML mice and then to investigate the effects of mangiferin on immune responses and survival rate of AML mice in vivo. The experiments were divided into two parts; one part is focused on immune responses, and the other part is the survival rate of leukemia mice after exposure to mangiferin.…”

“…Therefore, many studies attempted to find compounds from natural products to enhance the immune responses of leukemia patients. Mangiferin presents various pharmacological activity, including anti‐cancer activity in many human cancer cell lines 12‐14,16‐24,28 . In the present study, we used murine acute myelomonocytic leukemia WEHI‐3 cells to generate AML mice and then to investigate the effects of mangiferin on immune responses and survival rate of AML mice in vivo.…”

“…7 Mangiferin (1,3,6,7-tetrahydroxyxanthone-C2-β-D-glucoside), a flavonoid, was isolated from leaves and stem bark of Mangifera indica 8,9 and fruit peels and root of Mangifera indica L. 10 Mangiferin has been shown to present anti-inflammatory, 11,12 antioxidant, 13 antiviral 14 and antidiabetic 15 , and radioprotective 16 activities. Mangiferin was attractive for its anti-cancer activities in many cancer cell lines such as breast cancer, 17 colon cancer, 18 gastric carcinoma, 19 leukemia, 20 lung cancer, 21 multiple myeloma, 22 ovarian adenocarcinoma 23 , and prostate cancer. 24 Recently, the role of mangiferin in the immune responses has been focused and gained more attention and literature also reported that mangiferin exhibits immunomodulatory activities.…”

“…Mangiferin has been shown to present anti‐inflammatory, 11,12 antioxidant, 13 antiviral 14 and antidiabetic 15 , and radioprotective 16 activities. Mangiferin was attractive for its anti‐cancer activities in many cancer cell lines such as breast cancer, 17 colon cancer, 18 gastric carcinoma, 19 leukemia, 20 lung cancer, 21 multiple myeloma, 22 ovarian adenocarcinoma 23 , and prostate cancer 24 …”