Manipulation of electroosmotic flow in capillary electophoresis

Yao, Xian-Wei; Wu, Dan; Regnier, Fred E.

doi:10.1016/0021-9673(93)80052-a

Cited by 67 publications

(16 citation statements)

References 33 publications

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“…20,21 Cationic coating of the capillary eliminates analyte adsorption while providing an EOF compatible with nanospray CZE/ ES-MS. For these reasons, a series of coatings was investigated for use with the nanospray CZE/ES-MS interface.…”

Section: Capillary Coating and Separation Efficiencymentioning

confidence: 99%

Disposable Emitters for On-line Capillary Zone Electrophoresis/Nanoelectrospray Mass Spectrometry

Bateman

White

Thibault

1997

Rapid Commun. Mass Spectrom.

116

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A new method for preparing disposable microsprayers, for on-line capillary-zone electrophoresis/electrospray mass spectrometry (CZE/ES-MS) at sub-microliter per minute flow rates, is described. Different means of coupling these microsprayers to the CZE columns are presented, and compared with the more traditional one-piece capillary arrangement used for on-line CZE/ES-MS analysis. Enhancement of separation performance was obtained using capillaries coated with [(acryloylamino) propyl]trimethylammonium chloride reagents, an attractive alternative to other amine-coated capillaries. The use of disposable microsprayers provided ease of operation and flexibility for on-line CZE/ES-MS experiments, since CZE columns can be prepared independently from the microsprayers. The application of these microsprayers to unattended CZE/ES-MS operation for more than 8 hours was evaluated, and found to provide relative standard deviation values of 3-8% on peak areas and less than 1% on migration times. Full-scan mass spectral acquisition was obtained for low femtomol injections of peptides. The technique was applied to the analysis of a single chain lectin from Phaseolus vulgaris, using both CZE/ES-MS and CZE combined with tandem mass spectrometry. The mass spectral data obtained in this way provided information on the structure of glycopeptides identified in digests of the glycoprotein.

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Section: Capillary Coating and Separation Efficiencymentioning

confidence: 99%

Disposable Emitters for On-line Capillary Zone Electrophoresis/Nanoelectrospray Mass Spectrometry

Bateman

White

Thibault

1997

Rapid Commun. Mass Spectrom.

116

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“…Yao and Regnier et al [29,30] manipulated the EOF by absorbing several surfactants and polymers including methylcellulose (MC)-15, MC-25, polyvinyl alcohol and poly(vinylpyrrolidine) to the octadecylsilane-derived capillary inner surface. The MC-25 coated capillary was found to have more than twice the resolution to any others, while the polyvinyl alcohol coating provided a faster separation.…”

Section: Ciefmentioning

confidence: 99%

CE‐based analysis of hemoglobin and its applications in clinical analysis

et al. 2006

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This review focuses on the developments and trends in CE including CIEF, CZE, MEKC, two-dimensional conjunction of CIEF-capillary gel electrophoresis, and MEKC-CZE on microfluidic devices coupled to different detection approaches, such as UV absorbance, LIF, MS, and chemiluminescence etc. for performing analysis of hemoglobin (Hb), also with an emphasis on its applications in clinical analysis. Analysis of human Hb is of important clinical sense for numerous hemoglobinopathies associated with the congenital defects and abnormal contents of Hb. The diversiform modes render CE a comprehensive primary clinical tool for Hb analysis, which is rapid, sensitive, high-resolution, and not labor-intensive.

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“…Next to the screening of libraries and the calculation of binding constants, affinity capillary electrophoresis can also be used for the calculation of the binding stoichiometries, the estimation of protein charges, and study of the association and dissociation kinetics (K d = K off /K on ) [33,34]. Even enzyme-catalyzed reactions have been characterized by ACE [35,36].…”

Section: Theorymentioning

confidence: 99%