Mapping and candidate gene identification of loci determining tolerance to greenbug (Schizaphis graminum, Rondani) in barley

Tocho, E.; Börner, Andreas; Lohwasser, U.; Castro, Ana María

doi:10.1007/s10681-012-0740-y

Cited by 8 publications

(8 citation statements)

References 32 publications

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“…The ISSR marker for resistance QTL1 on chromosome 2H was less precise in the Barke than in the Lina genetic background when validated in BC 2 F 1 DH lines, probably due to it being located less distally, at 13.1 cM (Cheung et al 2010 ), than the SNP markers from the present study at 0–6 cM. Barley chromosomes 2H and 3H may harbor QTL for resistance to RWA and GB as well, but their locations differ from those conferring resistance to R. padi (Nieto-Lopez and Blake 1994 ; Mittal et al 2008 ; Cheung et al 2010 ; Tocho et al 2012 , 2013 ; Azhaguvel et al 2014 ; Dahleen et al 2015 ).…”

Section: Discussionmentioning

confidence: 58%

Introgression of resistance to Rhopalosiphum padi L. from wild barley into cultivated barley facilitated by doubled haploid and molecular marker techniques

Åhman

Bengtsson

2019

Theor Appl Genet

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Key message Long-term pre-breeding using Hordeum vulgare ssp. spontaneum as a donor of bird cherry-oat aphid resistance has resulted in agronomically improved resistance sources of barley along with easy-to-use molecular markers. Abstract Bird cherry-oat aphid ( Rhopalosiphum padi L.) is a pest and a virus vector in barley to which there are no bred-resistant cultivars. The present study describes how resistance from Hordeum vulgare ssp. spontaneum has been introgressed in cultivated barley via five successive crosses with the same cultivar Lina (BC) and in parallel with other more modern barley cultivars. Most of the selections for resistance are based on measurements of individual aphid growth in the laboratory. This very slow phenotyping method has been complemented by molecular marker evaluation and application in part of the breeding material. Doubled haploid production in each generation has been crucial for more precise selection of lines with the quantitatively expressed resistance. A field trial of selected “BC 3 ”-generation lines essentially confirmed the laboratory results, so did genotyping of the whole pedigree of parents and selected “BC 2 ” and “BC 4 ” offspring lines. The Infinium iSelect 50 K SNP assay confirmed relationships between lines and discerned several new markers for a resistance QTL on chromosome 2H. Electronic supplementary material The online version of this article (10.1007/s00122-019-03287-3) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 58%

Introgression of resistance to Rhopalosiphum padi L. from wild barley into cultivated barley facilitated by doubled haploid and molecular marker techniques

Åhman

Bengtsson

2019

Theor Appl Genet

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“…As we used a different, qualitative method, it is likely that the greenbug resistance in Post 90*4/R015 from the present study and the Oregon Wolfe Barley from Tocho et al. () is controlled by different mechanisms. It is also likely that the virulence of the greenbugs used in the two studies was different, which is common among natural greenbug populations (Weng et al.…”

Section: Discussionmentioning

confidence: 64%

“…Tocho et al. () found that greenbug tolerance in ‘Oregon Wolfe Barley’ was controlled by three quantitative trait loci (QTL) that were located in chromosome 2H (for foliage area and dry weight), 5H (for final dry weight) and 7H (for chlorophyll content). As we used a different, qualitative method, it is likely that the greenbug resistance in Post 90*4/R015 from the present study and the Oregon Wolfe Barley from Tocho et al.…”

Section: Discussionmentioning

confidence: 99%

Molecular mapping of greenbug (Schizaphis graminum) resistance gene Rsg1 in barley

Azhaguvel¹,

Mornhinweg²,

Vidya-Saraswathi³

et al. 2014

Plant Breeding

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The greenbug, Schizaphis graminum (Rondani) is an extremely damaging aphid pest of barley (Hordeum vulgare L.) particularly in the southern Great Plains of the USA. The simply inherited, dominant resistance gene Rsg1 is in all greenbug-resistant US barley cultivars. In this study, we conducted molecular mapping of Rsg1 using an F 2:3 population derived from a cross between the greenbug-resistant Post 90*4/R015 and susceptible CI2260 inbred lines. Segregation of host responses to greenbug biotype E infestation confirmed that a single dominant gene is responsible for greenbug resistance in Post 90*4/R015. Simple sequence repeat (SSR) markers evenly distributed along the seven barley chromosomes were employed for the construction of a framework genetic map. Linkage analysis placed the Rsg1 locus in the long arm of chromosome 3H (3HL) flanked by SSR markers Bmag0877 and GBM1420 that were 35 cM apart. Polymorphic single-nucleotide polymorphism (SNP) markers in 3HL were identified from an Illumina GoldenGate SNP assay and used for targeted mapping to locate Rsg1 to an 8.4-cM interval. Comparative analysis identified syntenic genomic regions in Brachypodium distachyon chromosome 2, in which 37 putative genes were annotated including a NB-LRR-type resistance gene homologue that may be a potential candidate gene for the Rsg1 locus of barley. Results from this study offer a starting point for fine mapping and cloning of this aphid resistance gene in barley.

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“…QTL analyses and genome-wide association mapping of RWA resistance in barley have revealed several responsible loci; among them loci on chromosome 2H and 3H (Nieto-Lopez and Blake 1994;Mittal et al 2008Mittal et al , 2009Tocho et al 2012Tocho et al , 2013Dahleen et al 2015). The RWA resistance loci on 2H is at 27 and 39 cM (Mittal et al 2008;Mittal et al 2009, respectively), 18 and 20 cM (Dahleen et al 2015), 70-96 cM (Tocho et al 2012) and at 70-96 cM for GB resistance as well (Tocho et al 2013) and thus not at the same locus as the present QTL for R. padi resistance. The RWA resistance locus on 3H is at around 147 and 108 cM (Mittal et al 2008(Mittal et al , 2009 and 136 cM (Dahleen et al 2015), potentially within the region of the gramine QTL in our study.…”

Section: Discussionmentioning

confidence: 80%

Quantitative trait locus for resistance to the aphid Rhopalosiphum padi L. in barley (Hordeum vulgare L.) is not linked with a genomic region for gramine concentration

Macaulay

Ramsay

Åhman

2019

Arthropod-Plant Interactions

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Gramine is an indole alkaloid found in certain grass species. Results of previous studies have diverged as to whether this compound might cause resistance to aphids or not. In a breeding program aiming to introduce resistance to the cereal pest Rhopalosiphum padi L. in barley, a cultivar has been crossed with the progenitor of cultivated barley, Hordeum vulgare ssp. spontaneum, and an F 1-derived population of doubled haploid (DH) lines was screened both for seedling gramine concentration and resistance to R. padi. The resistance was measured as individual aphid growth in the laboratory. The present study aims to determine if there is a genetic relationship between aphid growth retardation and gramine concentration. To do so, the lines were genotyped with a 384 SNP oligonucleotide pool assay and QTL analyses were performed for both traits. A previously identified aphid resistance locus on the distal part of chromosome 2HS was thereby confirmed, with resistance inherited from H. v. ssp. spontaneum, whereas one or more QTL for gramine concentration were potentially indicated on chromosome 3H, thus corroborating that the two traits are not linked genetically.

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Mapping and candidate gene identification of loci determining tolerance to greenbug (Schizaphis graminum, Rondani) in barley

Cited by 8 publications

References 32 publications

Introgression of resistance to Rhopalosiphum padi L. from wild barley into cultivated barley facilitated by doubled haploid and molecular marker techniques

Introgression of resistance to Rhopalosiphum padi L. from wild barley into cultivated barley facilitated by doubled haploid and molecular marker techniques

Molecular mapping of greenbug (Schizaphis graminum) resistance gene Rsg1 in barley

Quantitative trait locus for resistance to the aphid Rhopalosiphum padi L. in barley (Hordeum vulgare L.) is not linked with a genomic region for gramine concentration

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