Mapping gene H controlling cane pubescence in raspberry and its association with resistance to cane botrytis and spur blight, rust and cane spot

Abstract

Disease resistance is increasing in importance, as consumers require high-quality raspberry fruit at a time when chemical disease control is undesirable. Breeders have limited resources and rarely include a primary screen for each fungal disease. Marker-assisted breeding would facilitate the introduction of resistance into elite germplasm and breeding lines. An additional 20 simple sequence repeats have been added to the existing raspberry linkage map. Gene H, which determines cane pubescence (genotype HH or Hh), the recessive allele of which gives glabrous canes (genotype hh), has been mapped on to linkage group 2 and shown to be closely associated with resistance to cane botrytis and spur blight but not rust or cane spot. Other map regions on linkage groups 3, 5 and 6 associated with disease resistance are reported here.

Environmental and seasonal influences on red raspberry flavour volatiles and identification of quantitative trait loci (QTL) and candidate genes

“…An evolving genetic linkage map from this reference population was available (Graham et al 2004(Graham et al , 2006 Kassim et al 2009;McCallum et al 2010;Woodhead et al 2010Woodhead et al , 2012. Genes related to volatile regulation were identified and were mapped when polymorphism was detected.…”

Characterisation of Gene H in Red Raspberry: Explaining its Role in Cane Morphology, Disease Resistance and Timing of Fruit Ripening

“…The mapping population, as described previously [1,9,13,14] used to identify the Gene H region, consists of a full sib family generated from a cross between the European red raspberry cv. Glen Moy (genotype Hh) and the North American red raspberry cv.…”

“…The Gene H region on raspberry linkage group 2 is interesting as it is associated with a number of diverse traits [1]. It has been known for some time that pubescence in raspberry is determined by Gene H (genotype HH or Hh), the recessive allele of which gives glabrous canes (genotype hh).…”

“…than non-hairy ones [2,3] but more susceptible to cane spot (Elsinoe veneta), powdery mildew (Sphaerotheca macularis) and yellow rust (Phragmidium rubi-idaei) [4][5][6][7][8]. Gene H was shown to be the most significant marker in a QTL on Linkage Group (LG) 2 that control cane pubescence (hairs) and disease resistances [1] and also delayed ripening [9].…”

“…An alternative explanation is that the gene itself is responsible through pleiotrophic effects on each of the resistances [10]. The gene is known to have other effects besides its main effect on cane pubescence: it is associated with a small increase in spine frequency and decrease in spine size [1,4,11]. Hairs and spines are both outgrowths of epidermal cells and their early development is inter-related [12].…”