Mapping of yellow mosaic virus (YMV) resistance in soybean (Glycine max L. Merr.) through association mapping approach

Abstract

Yellow Mosaic Virus (YMV) is a serious disease of soybean. Resistance to YMV was mapped in 180 soybean genotypes through association mapping approach using 121 simple sequence repeats (SSR) and four resistance gene analogue (RGA)-based markers. The association mapping population (AMP) (96 genotypes) and confirmation population (CP) (84 genotypes) was tested for resistance to YMV at hot-spot consecutively for 3 years (2007-2009). The genotypes exhibited significant variability for YMV resistance (P < 0.01). Molecular genotyping and population structure analysis with 'admixture' co-ancestry model detected seven optimal sub-populations in the AMP. Linkage disequilibrium (LD) between the markers extended up to 35 and 10 cM with r2 > 0.15, and >0.25, respectively. The 4 RGA-based markers showed no association with YMV resistance. Two SSR markers, Satt301 and GMHSP179 on chromosome 17 were found to be in significant LD with YMV resistance. Contingency Chi-square test confirmed the association (P < 0.01) and the utility of the markers was validated in the CP. It would pave the way for marker assisted selection for YMV resistance in soybean. This is the first report of its kind in soybean.

LRRK2 at the interface of autophagosomes, endosomes and lysosomes

“…Since the initial cloning of SNCA , there have been multiple PD-related genes identified that additionally converge on the related autophagy-lysosome system and vesicle trafficking pathways (summarized in Table 1, extensively reviewed in [28, 32]). We will therefore summarize some of the key characteristics and players in these intracellular events before turning to the evidence that addresses the role(s) of LRRK2 in vesicle uptake and recycling.…”

Linkage mapping of <i>Mungbean yellow mosaic India virus</i> (MYMIV) resistance gene in soybean

“…They indicated a Single Nucleotide Polymorphism (SNP) on chr 18 (LG G) with a possible association with MYMIV resistance gene. Kumar et al (2015) reported a region on chr 17 (LG D2) in significant linkage disequilibrium with resistance gene in association mapping study. But none of the reported linkage has been validated in mapping population.…”

Mapping of Duplicate Dominant Genes for Mungbean yellow mosaic India virus Resistance in Glycine soja

“…In that study, a single‐nucleotide polymorphism on chromosome 18 showed a possible association with a MYMIV resistance gene. Kumar et al (2015) found a region on chromosome 17 in significant linkage disequilibrium with MYMIV resistance in an association mapping study. Recently Rani et al (2017) mapped a MYMIV resistance gene on chromosome 6 very close to the two SSR markers Satt322 and GMAC7L.…”

“…In that study, a single-nucleotide polymorphism on chromosome 18 showed a possible association with a MYMIV resistance gene. Kumar et al (2015) found a region on chromosome 17 in significant linkage disequilibrium with MYMIV resistance in an association mapping study.…”

“…Backcrossing without a marker linked closely to a desirable trait is very difficult because segregating material that is generated for introgressing an MYMIV resistance gene into high‐yielding adapted cultivars must be screened at hot spots or under artificial conditions. Several reports of molecular markers linked to MYMIV resistance in G. max are available (Yadav et al, 2015; Kumar et al, 2015; Rani et al, 2017), and the linked markers identified by Rani et al (2017) are being actively deployed for introgression of an MYMIV resistance gene into the predominant cultivars used in central India. However, no attempt has been made to map MYMIV resistance gene(s) in G. soja .…”