Linkage mapping of <i>Mungbean yellow mosaic India virus</i> (MYMIV) resistance gene in soybean

Abstract

Mungbean Yellow Mosaic India Virus (MYMIV) is one of the most prevalent pathogen that limits soybean production in India. In this study RILs derived from JS335, dominant but MYMIV susceptible variety and PI171443, donor of MYMIV resistance gene in most of the MYMIV resistant varieties released in India and F2 population derived from SL525, a resistant variety released for northern India and NRC101, a susceptible genotype were used to study the inheritance of MYMIV resistance and map the gene responsible for MYMIV resistance. F1s were found to be completely susceptible. F2:3 and RILs population segregated to fit a ratio of 1:2:1 and 1:1 indicating that a single recessive gene controlled resistance to MYMIV. BSA was performed using 144 polymorphic SSR markers. MYMIV resistance gene was mapped on chr 6 (LG C2) within a 3.5-cM genome region between two SSR markers GMAC7L and Satt322 whose size was estimated to be 77.115 kb (position of 12,259,594–12,336,709 bp). This is the first report on linkage mapping of MYMIV resistance gene in soybean. This will be helpful in breeding soybean varieties for resistance against MYMIV responsible for wide spread damage to soybean crop in India using Marker Assisted Selection.

Genotypic Analysis for Retrieval of MYMV Resistant Progenies from Certain Crosses of Mungbean [Vigna radiata (L.) Wilczek]

“…Crossing is made with susceptible and resistance parents to study the inheritance of MYMV resistance. In first filial generation, the plants of all the crosses showed susceptible symptom indicating that susceptibility was dominant over resistance (Narasimhulu et al, 2016, Webster et al, 20004) Similar results on the dominant behavior of MYMV susceptibility in the F 1 were also reported in the mungbean by Khan et al (2007) and Rani et al (2017). To deduce the inheritance pattern of MYMV resistance, eight first filial crosses were forwarded to second filial generation for the study of segregating pattern.…”

Mapping of Duplicate Dominant Genes for Mungbean yellow mosaic India virus Resistance in Glycine soja

“…Kumar et al (2015) found a region on chromosome 17 in significant linkage disequilibrium with MYMIV resistance in an association mapping study. Recently Rani et al (2017) mapped a MYMIV resistance gene on chromosome 6 very close to the two SSR markers Satt322 and GMAC7L. As viruses continuously mutate to evade resistance mechanisms of host plants, it is essential to continue to map MYMIV resistance genes from new sources to meet future challenges.…”

“…Further studies are required to investigate whether the MYMIV resistance genes mapped in this study are related to the same gene family. The SSR markers Satt322 and GMAC7L, previously reported as linked to MYMIV resistance in G. max (Rani et al, 2017), are being successfully deployed to introgress a MYMIV resistance gene from PI 171443 into the predominant cultivated varieties of central India. However, breakdown of resistance to plant disease can frequently occur, particularly when the resistance to a specific disease is conditioned by a single gene.…”

“…Backcrossing without a marker linked closely to a desirable trait is very difficult because segregating material that is generated for introgressing an MYMIV resistance gene into high‐yielding adapted cultivars must be screened at hot spots or under artificial conditions. Several reports of molecular markers linked to MYMIV resistance in G. max are available (Yadav et al, 2015; Kumar et al, 2015; Rani et al, 2017), and the linked markers identified by Rani et al (2017) are being actively deployed for introgression of an MYMIV resistance gene into the predominant cultivars used in central India. However, no attempt has been made to map MYMIV resistance gene(s) in G. soja .…”

“…Several studies have examined the inheritance of MYMIV resistance in G. max. Rani et al (2017) reported MYMIV resistance controlled by a single recessive gene in PI 171443, Singh and Mallick (1978) reported MYMIV resistance controlled by double recessive genes in PI 171443, and Talukdar et al (2013) reported MYMIV resistance controlled by a single dominant gene in YMDresistant cultivars 'DS97-12' and 'DS98-14'. One report by Bhattacharyya et al (1999) indicated a single dominant gene controlling YMD resistance gene in G. soja accession PI 393551; however, this result has not been further validated.…”