Soybean is a major source of high quality protein and oil and soybean seed quality is often determined by seed nutritional and antinutritional parameters.
Mungbean Yellow Mosaic India Virus (MYMIV) is one of the most prevalent pathogen that limits soybean production in India. In this study RILs derived from JS335, dominant but MYMIV susceptible variety and PI171443, donor of MYMIV resistance gene in most of the MYMIV resistant varieties released in India and F2 population derived from SL525, a resistant variety released for northern India and NRC101, a susceptible genotype were used to study the inheritance of MYMIV resistance and map the gene responsible for MYMIV resistance. F1s were found to be completely susceptible. F2:3 and RILs population segregated to fit a ratio of 1:2:1 and 1:1 indicating that a single recessive gene controlled resistance to MYMIV. BSA was performed using 144 polymorphic SSR markers. MYMIV resistance gene was mapped on chr 6 (LG C2) within a 3.5-cM genome region between two SSR markers GMAC7L and Satt322 whose size was estimated to be 77.115 kb (position of 12,259,594–12,336,709 bp). This is the first report on linkage mapping of MYMIV resistance gene in soybean. This will be helpful in breeding soybean varieties for resistance against MYMIV responsible for wide spread damage to soybean crop in India using Marker Assisted Selection.
This investigation was conducted in order to determine the degree of biochemical changes during natural ageing of soybean seeds borne on different positions on stem axis. Biochemical analysis of field grown soybean seeds revealed significant differences in their oil and protein contents as a function of nodal positions. Both oil and protein content decreased after 180 days of storage (DOS) at all the nodal positions. Proportions of membrane lipids in seeds were less in basal as compared to apical positions and their proportion in stored seeds also decreased with increasing storage periods. The content of starch, total soluble sugars and reducing sugars in seeds decreased during storage for 180 days but it didn't show positional variations in their contents. The extent of lipid peroxidation increased during storage was associated with the corresponding decrease in the activities of antioxidant enzymes viz catalase and peroxidase in seeds. Seeds at basal positions showed higher rate of lipid peroxidation and lower peroxidase activity as compared to apical positions. Results suggest that soybean seeds collected from basal positions showed higher deteriorative changes during storage in comparison to apical positions that might be related to higher lipid content in seeds from basal portion of soybean stem axis as compared to apical portion.
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