Tuberculosis (TB) remains a significant public health challenge globally, with Indonesia being the second-highest country in TB cases worldwide. Aceh Province has one of the highest TB incidence rates in Indonesia. This study aims to estimate and map the spatial distribution patterns of TB relative risk across districts in Aceh Province, Indonesia, to reveal significant variations. The study employed an ecological time-series study design, utilizing the Bayesian Conditional Autoregressive (CAR) approach with the Besag-York-Mollie (BYM) model for spatial estimation and mapping of TB relative risk. TB case data and population data for 23 districts/cities in Aceh Province from 2016 to 2022 were analyzed. Spatial analysis was used to estimate and map TB's relative risk, aiding in identifying areas with higher transmission risks. The results showed that the relative risk of TB varied across districts/cities in Aceh Province over the study period. However, Lhokseumawe and Banda Aceh consistently exhibited high to very high relative risks over the years. In 2022, Lhokseumawe City and Banda Aceh City had the highest relative risks by 2.26 and 2.17, respectively, while Sabang City and Bener Meriah District had the lowest by 0.43 and 0.32, respectively. This study provides valuable insights into the heterogeneous landscape of TB risk in Aceh Province, which can inform targeted interventions and planning strategies for effective TB control. Using the Bayesian CAR BYM model proved effective in estimating and mapping TB's relative risk, highlighting areas requiring prioritized attention in TB prevention and control efforts.