Mapping the Proteome of Barrel Medic (<i>Medicago truncatula</i>),

Watson, Bonnie S.; Asirvatham, Victor S.; Wang, Liangjiang; Sumner, Lloyd W.

doi:10.1104/pp.102.019034

Cited by 212 publications

(168 citation statements)

References 78 publications

(90 reference statements)

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“…Protein separation by 2-DE is known to have physical limitation in separating proteins, where proteins of extreme pIs and Mr are excluded from the 2-DE profiles [42,43]. Limited Mr range of 2-DE electrophoresed spots has also been reported in other studies of the proteome of maize (Zea mays) leaves [44] and cell suspension cultures [45] as well as different tissues [46] of barrel medic (Medicago truncatula), amongst others. The IEF pH restriction of 4-7 in sorghum leaf proteome has also been previously reported [23].…”

Section: -De Image Analysis and Annotation Of Stress-responsive Protmentioning

confidence: 89%

Identification and profiling of salinity stress-responsive proteins in Sorghum bicolor seedlings

Ngara

Ndimba

Borch-Jensen

et al. 2012

Journal of Proteomics

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Sorghum bicolor, a drought tolerant cereal crop, is not only an important food source in the semi arid/arid regions but also a potential model for studying and gaining a better understanding of the molecular mechanisms of drought and salt stress tolerance in cereals. In this study, seeds of a sweet sorghum variety, MN1618, were planted and grown on solid MS growth medium with or without 100 mM NaCl. Heat shock protein expression immunoblotting assays demonstrated that this salt treatment induced stress within natural physiological parameters for our experimental material. 2D PAGE in combination with MS/MS proteomics techniques were used to separate, visualise and identify salinity stress responsive proteins in young sorghum leaves. Out of 281 Coomassie stainable spots, 118 showed statistically significant responses (p<0.05) to salt stress treatments. Of the 118 spots, 79 were selected for tandem mass spectrometric identification, owing to their good resolution and abundance levels, and of these, 55 were positively identified. Identified proteins were divided into six functional categories including both known and novel/putative stress responsive proteins. Molecular and physiological functions of some of our proteins of interest are currently under investigation via bioinformatic and molecular biology approaches.

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Section: -De Image Analysis and Annotation Of Stress-responsive Protmentioning

confidence: 89%

Identification and profiling of salinity stress-responsive proteins in Sorghum bicolor seedlings

Ngara

Ndimba

Borch-Jensen

et al. 2012

Journal of Proteomics

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“…Proteomic investigations into nodule biology complement parallel transcription-profiling studies by revealing subtleties that might reflect differential levels of gene regulation. Soybean, Lotus and Medicago have been the subject of numerous proteomic studies [61][62][63]64 ]. These studies were targeted to various tissues during development or under biotic and abiotic elicitation.…”

Section: Database (Url)mentioning

confidence: 99%

Genetics and functional genomics of legume nodulation

Stacey

Libault

Brechenmacher

et al. 2006

Current Opinion in Plant Biology

252

138

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Gram-negative soil bacteria (rhizobia) within the Rhizobiaceae phylogenetic family (a-proteobacteria) have the unique ability to infect and establish a nitrogen-fixing symbiosis on the roots of leguminous plants. This symbiosis is of agronomic importance, reducing the need for nitrogen fertilizer for agriculturally important plants (e.g. soybean and alfalfa). The establishment of the symbiosis involves a complex interplay between host and symbiont, resulting in the formation of a novel organ, the nodule, which the bacteria colonize as intracellular symbionts. This review focuses on the most recent discoveries relating to how this symbiosis is established. Two general developments have contributed to the recent explosion of research progress in this area: first, the adoption of two genetic model legumes, Medicago truncatula and Lotus japonicus, and second, the application of modern methods in functional genomics (e.g. transcriptomic, proteomic and metabolomic analyses). IntroductionNodulation is a highly host-specific interaction in which, with few exceptions, specific rhizobial strains infect a limited range of plant hosts. Plants secrete (iso)flavonoids that are recognized by the compatible bacteria, resulting in the induction of nodulation genes. These nodulation genes encode enzymes that synthesize a specific lipochitin nodulation signal (Nod signal), which activates many of the early events in the root hair infection process [1][2][3][4][5]. During the infection process, the bacteria enter the plant via the root epidermis and induce the reprogramming of root cortical cell development and the formation of a nodule. In the most well-studied cases, infection occurs through root hairs. The first observable event in the infection process is the curling of the root hair, which likely occurs through the gradual and constant reorientation of the direction of root hair growth. The bacteria become enclosed within the root hair curl where the plant cell wall is degraded, the cell membrane is invaginated and an intracellular tubular structure (i.e. the infection thread) is initiated. It is within this infection thread that the bacteria enter the root hair cell and eventually ramify into the root cortex. Before the infection thread reaches the base of the root hair cell, the root cortical cells are induced to de-differentiate, activating their cell cycle and causing them to divide to form the nodule primordium. In addition to the cortical cells, pericycle cells are also activated and undergo some cell divisions. When the infection thread reaches the cells of the developing primordium, the bacteria are released into cells via endocytosis. Inside a plant cell, the bacteria are enclosed in vacuole-like structures (symbiosomes) in which they differentiate into bacteroids. It is within these symbiosomes that the bacteria convert N 2 to NH 3 . The nodule is a true organ in which there is cellular specialization. For example, in addition to infected plant cells, uninfected plant cells also carry out the function of nitrogen as...

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“…Several proteomic approaches on PM preparations from tobacco (Nicotiana tabacum) leaves and Arabidopsis (Arabidopsis thaliana) seedlings now suggest localization of remorins in association with PMs (Watson et al, 2003;Marmagne et al, 2004;Mongrand et al, 2004;Sazuka et al, 2004;Nelson et al, 2006;Valot et al, 2006). Interestingly, remorins have also been found in detergent-resistant membrane fractions, called lipid rafts (Mongrand et al, 2004;Shahollari et al, 2004;Bhat and Panstruga, 2005;Laloi et al, 2007;Lefebvre et al, 2007).…”

mentioning

confidence: 99%