Mapping the Sw-5 locus for tomato spotted wilt virus resistance in tomatoes using RAPD and RFLP analyses

Stevens, Mikel R.; Lamb, Elizabeth; Rhoads, David B.

doi:10.1007/bf00221989

Cited by 95 publications

(59 citation statements)

References 30 publications

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“…Many disease resistance genes have been identified in tomato using random amplified polymorphic DNA (RAPD) following the BSA approach (Stevens et al 1995, Chagué et al 1996, De Giovanni et al 2004). Once it is identified, this marker is then converted into co-dominant markers, such as cleaved amplified polymorphic sequence (CAPS), sequence characterized amplified region (SCAR) which are more reliable than RAPDs.…”

Section: Introductionmentioning

confidence: 99%

RAPD Markers Linked to Late Blight Resistance in Tomato

Joshi

Panthee

Louws

et al. 2013

Nepal Journal of Science and Technology

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Identification of marker is the prerequisite for marker assisted selection (MAS) and is also very effective for gene pyramiding. Bulked Segregant Analysis (BSA) technique was used to identify RAPD markers linked to the late blight disease (caused by Phytopthora infestans (Mont. de Bary) resistance in tomato using F 2 population generated by crossing tomato inbred lines, NC 85L-1W (2007) which is resistant to late blight and NC 839-2(2007)-1 which is susceptible to it. A total of 250 F 2 plants, and 10 plants each of the parents and F 1 were used for BSA. Transgressive segregation was observed for late blight resistance. The segregation of susceptible and resistance perfectly fit the expected ratio of 3:1, that means resistance is governed by single recessive gene. Only 34 RAPD primers (17.26%) were found polymorphic between parents. Sixteen RAPD primers (47%) out of 34 gave polymorphic bands between resistant and susceptible bulks of the late blight. Four RAPD primers, namely MRTOMR-026, MRTOMR-031, MRTOMR-038 and MRTOMR-046 were identified as linked markers to loci related to late blight disease. Among those, two were linked to susceptible and two to resistance. Because of low reproducibility and dominant nature of RAPD, these markers need to be converted to SCAR markers.

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Section: Introductionmentioning

confidence: 99%

RAPD Markers Linked to Late Blight Resistance in Tomato

Joshi

Panthee

Louws

et al. 2013

Nepal Journal of Science and Technology

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“…The gene Sw-5 is closely linked to the restriction fragment length polymorphism (RFLP) markers CT220 and CT71 and is located near the telomere of chromosome 9 (Stevens et al 1995). Sw-5 is a member of a loosely clustered gene family, members of which have been genetically mapped close to Sw-5, to other regions of chromosome 9, and to chromosome 12 (Brommonschenkel et al 2000).…”

Section: Introductionmentioning

confidence: 99%

The development of an improved PCR-based marker system for Sw-5, an important TSWV resistance gene of tomato

Garland¹,

Sharman²,

Persley³

et al. 2005

Aust. J. Agric. Res.

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Abstract. Sw-5 is an important disease resistance gene of tomato, providing broad resistance to Tomato spotted wilt virus (TSWV). A cleaved amplified polymorphic sequence (CAPS) marker, closely linked to the gene, has been reported. Although the Sw-5 locus has been characterised, a gene-specific marker has not been developed. This paper presents a PCR-based marker-system that consists of the co-amplification of a dominant marker representing the Sw-5 gene sequence, and the modified CAPS marker as a positive control and indicator of genotype.Additional keyword: rapid DNA extraction.

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“…Stevens et al (1995) using a near isogenic line developed a RAPD marker of the size of 2200bp (base pare) linked to Sw-5 gene. They determined the location of the gene for resistance to TSWV to be between the RFLP CT71 and CT220 markers, near the telomere on the short arm of chromosome 9.…”

Section: Viral Diseasementioning

confidence: 99%

Tomato Molecular Markers

Szczechura

Staniaszek

Habdas

2011

Journal of Fruit and Ornamental Plant Research

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SummaryTomato (Solanum lycopersicum L.) is one of the most popular vegetable grown in many regions of the world. Due to its high taste quality and nutritional value increase interest in the cultivation of this species and its consumption. Using the latest achievements in fields of genetics, molecular biology and biotechnology, breeders can create new varieties with improved useful traits. Introduction of DNA markers, especially those based on the polymerase chain reaction (PCR) has led to breakthrough in the plants genetic research, including tomato. They are successfully used for plant genomes mapping, phylogenetics studies, selection of parental forms in plant breeding, and above all to identify the genes of important traits. For tomato have been identified and mapped 9309 molecular markers. High-density genetic maps development gives an opportunity to use them in genetic research and breeding programs. Identification of DNA markers closely linked to studied gene can significantly facilitate the identification of desirable traits in material breeding, or accelerate the plants selection for elimination of genotypes with undesirable genes. Material breeding selection using molecular markers, defined as MAS (marker-assisted-selection) is increasingly being used in tomato breeding programs, contributing to facilitated identification of genes or QTL and their transfer into the cultivated species from wild form.

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Mapping the Sw-5 locus for tomato spotted wilt virus resistance in tomatoes using RAPD and RFLP analyses

Cited by 95 publications

References 30 publications

RAPD Markers Linked to Late Blight Resistance in Tomato

RAPD Markers Linked to Late Blight Resistance in Tomato

The development of an improved PCR-based marker system for Sw-5, an important TSWV resistance gene of tomato

Tomato Molecular Markers

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