Marcadores biológicos de necrosis miocárdica

Bel, Miguel Santaló; Soldevila, Josep Guindo; Llanos, Jordi Ordóñez

doi:10.1157/13049653

Cited by 8 publications

(5 citation statements)

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“…Los valores de concentración de CK-MB pueden variar por el método de inmunoanálisis utilizado (Santaló et al 2003). Pino (2006) utilizó el mismo método empleado en el presente estudio, lo cual se refleja en la similitud obtenida en las medias aritméticas (186 U/L), cuyo valor es de 181,4 U/L en el presente estudio.…”

Section: Discussionunclassified

“…Esto puede diferir en los pacientes con enfermedad valvular degenerativa en estadios C, los cuales requieren un tiempo de evolución de la enfermedad, generando dificultades en la utilización de CK-MB como biomarcador en medicina veterinaria. Es posible en pacientes humanos una rápida elevación y descenso, la CK-MB puede utilizarse para detectar un reinfarto ulterior (Santaló et al 2003). De allí, que se pueda asumir que es más probable que los valores séricos de CK-MB se encuentren aumentados en casos agudos, lo cual puede diferir en medicina veterinaria.…”

Section: Discussionunclassified

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Niveles séricos de la isoenzima creatina quinasa-MB y lactato deshidrogenasa como indicadores de daño miocárdico en perros con enfermedad valvular degenerativa

Sepúlveda

Paredes

Vásquez

2022

Rev. Med. Vet. Zoot.

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Se analizaron los niveles séricos de creatina quinasa-MB (CK-MB) y lactato deshidrogenasa (LDH) en 10 perros diagnosticados con enfermedad valvular degenerativa y en seis perros clínicamente sanos, con el objetivo de evaluar si sus niveles séricos indican daño miocárdico. Las muestras de suero se analizaron mediante el método UV. Se utilizó la prueba de diferenciación de medias para determinar diferencias entre medias, y la prueba de correlación de Pearson para determinar si existe correlación entre los niveles séricos de ambas enzimas. Los valores de CK-MB y de LDH fueron significativamente diferentes entre los dos grupos de pacientes. Los niveles de CK-MB y LDH tuvieron correlación positiva, pero no significativa.

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Section: Discussionunclassified

Niveles séricos de la isoenzima creatina quinasa-MB y lactato deshidrogenasa como indicadores de daño miocárdico en perros con enfermedad valvular degenerativa

Sepúlveda

Paredes

Vásquez

2022

Rev. Med. Vet. Zoot.

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“…Although some debate remains, the detection of proteins bound to intracellular structures (mitochondria, nucleus, etc), even in small amounts, is usually considered to indicate necrosis. 7 The molecular diagnosis of muscle damage is largely based on measurement of the plasma activity of different sarcoplasmatic enzymes (creatine kinase (CK) and lactate dehydrogenase (LDH)). These enzymes are normally strictly intracellular, and their increased activity in plasma reflects their escape via membrane structures.…”

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confidence: 99%

Role of α-actin in muscle damage of injured athletes in comparison with traditional markers

et al. 2007

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Objective: In order to identify a reliable marker for the early detection of muscle injuries in sports, a-actin protein and other markers of muscle damage were studied in sera of uninjured sportspeople and those with skeletal muscle injury. Methods: Blood samples were obtained from 20 sportspeople with skeletal muscle injury and 48 uninjured sportspeople. Immunoassays were performed to determine cardiac troponin I (TnI), troponin T, lactate dehydrogenase and myoglobin concentrations. Western blot and densitometry were used to measure a-actin concentrations. Skeletal muscle damage was diagnosed according to physical examination, MRI findings and the biochemical criterion of a creatine kinase value .500 IU/l (Rosalki method, Beckman Instruments SL, Fullerton, California, USA). Results were also compared with previously obtained data on injured and uninjured non-sportspeople. Results: The mean serum concentration of a-actin was significantly higher in sportspeople with muscle damage (10.49 mg/ml) than in uninjured sportspeople (3.99 mg/ml). Sera from injured sportspeople showed higher levels of a-actin than of troponin or myoglobin. No significant difference in TnI levels was observed between the groups. Conclusions: According to these results, a-actin is a new and reliable marker of skeletal muscle damage in sportspeople which can be used for the detection of muscle injury. Possible cross interference between skeletal and cardiac muscle damage can be discriminated by the combined use of a-actin and TnI. These data suggest that early measurement of a-actin in sportspeople with suspected muscle damage will allow them to receive earlier and more effective treatment and to return sooner to the practice of their sport.

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“…[5][6][7] An increase in the serum activity of these intracellular enzymes reflects their escape through the membrane of injured cells. 8 9 However, this method has some drawbacks.…”

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confidence: 99%

“…14 The detection of even small amounts of proteins bound to intracellular structures 15 (mitochondria, nucleus, cellular contractile apparatus) always indicates necrosis. 6 Thus, troponins and a-actin have been used as biochemical markers of heart damage. Subunits T, C, and I of troponin are proteins of the contractile apparatus found in different isoforms according to the muscle type.…”

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confidence: 99%

Release of α-actin into serum after skeletal muscle damage

et al. 2005

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Objective: The skeletal muscle protein α-actin was investigated in the serum of subjects with severe skeletal muscle damage to assess its utility as a reliable and predictive marker of muscle damage.Methods: Serum samples were obtained from 33 healthy controls and 33 patients with severe skeletal muscle damage, defined by a total creatine kinase value of >500 IU/l (Rosalki method). Troponin I, troponin T, and myoglobin concentrations were determined by immunoassay and α-actin concentrations by Western blot and densitometry.Results: The mean serum concentration of α-actin in controls and patients with skeletal muscle damage was 600.9 and 1968.51 ng/ml, respectively, a statistically significant difference. Sera of patients with muscle damage showed higher levels of α-actin than of troponin or myoglobin. No significant difference in troponin I levels was observed between the groups.Conclusions: According to these results, α-actin was the most significant skeletal muscle damage marker analysed and may be an ideal candidate for the identification of all types of myofibre injury, including sports injuries. Our findings support the use of α-actin as a marker alongside other currently used biological proteins.

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Marcadores biológicos de necrosis miocárdica

Cited by 8 publications

References 0 publications

Niveles séricos de la isoenzima creatina quinasa-MB y lactato deshidrogenasa como indicadores de daño miocárdico en perros con enfermedad valvular degenerativa

Niveles séricos de la isoenzima creatina quinasa-MB y lactato deshidrogenasa como indicadores de daño miocárdico en perros con enfermedad valvular degenerativa

Role of α-actin in muscle damage of injured athletes in comparison with traditional markers

Release of α-actin into serum after skeletal muscle damage

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