Marine cyanobacteria tune energy transfer efficiency in their light‐harvesting antennae by modifying pigment coupling

Kolodny, Yehoshua; Zer, Hagit; Propper, Mor; Yochelis, Shira; Paltiel, Yossi; Keren, Nir

doi:10.1111/febs.15371

Cited by 24 publications

(25 citation statements)

References 51 publications

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“…Further, the symbionts' fluorescence peak in the shallow and in the transplanted fragments was similar ( Figure 2D), meaning that the antennae to reaction centers ratio of the transplants has become similar to that of the shallow colonies. It was recently discovered, in marine cyanobacteria, that enhancement of photosynthetic efficiency could be achieved by tuning the coupling within the antennae complexes (Kolodny et al, 2020), a mechanism that might also be utilized in Symbiodiniaceae and could explain the observed differences in YII (Figure 2A).…”

Section: Discussionmentioning

confidence: 99%

Energy Sources of the Depth-Generalist Mixotrophic Coral Stylophora pistillata

et al. 2020

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Energy sources of corals, ultimately sunlight and plankton availability, change dramatically from shallow to mesophotic (30–150 m) reefs. Depth-generalist corals, those that occupy both of these two distinct ecosystems, are adapted to cope with such extremely diverse conditions. In this study, we investigated the trophic strategy of the depth-generalist hermatypic coral Stylophora pistillata and the ability of mesophotic colonies to adapt to shallow reefs. We compared symbiont genera composition, photosynthetic traits and the holobiont trophic position and carbon sources, calculated from amino acids compound-specific stable isotope analysis (AA-CSIA), of shallow, mesophotic and translocated corals. This species harbors different Symbiodiniaceae genera at the two depths: Cladocopium goreaui (dominant in mesophotic colonies) and Symbiodinium microadriaticum (dominant in shallow colonies) with a limited change after transplantation. This allowed us to determine which traits stem from hosting different symbiont species compositions across the depth gradient. Calculation of holobiont trophic position based on amino acid δ15N revealed that heterotrophy represents the same portion of the total energy budget in both depths, in contrast to the dogma that predation is higher in corals growing in low light conditions. Photosynthesis is the major carbon source to corals growing at both depths, but the photosynthetic rate is higher in the shallow reef corals, implicating both higher energy consumption and higher predation rate in the shallow habitat. In the corals transplanted from deep to shallow reef, we observed extensive photo-acclimation by the Symbiodiniaceae cells, including substantial cellular morphological modifications, increased cellular chlorophyll a, lower antennae to photosystems ratios and carbon signature similar to the local shallow colonies. In contrast, non-photochemical quenching remains low and does not increase to cope with the high light regime of the shallow reef. Furthermore, host acclimation is much slower in these deep-to-shallow transplanted corals as evident from the lower trophic position and tissue density compared to the shallow-water corals, even after long-term transplantation (18 months). Our results suggest that while mesophotic reefs could serve as a potential refuge for shallow corals, the transition is complex, as even after a year and a half the acclimation is only partial.

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Section: Discussionmentioning

confidence: 99%

Energy Sources of the Depth-Generalist Mixotrophic Coral Stylophora pistillata

et al. 2020

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“…Specifically, our cells were grown at 20 °C and 60 μmol photons m −2 s −1 temperature and irradiance, respectively, and under these same conditions, three Synechococcus strains displayed F v / F m values of 0.26–0.34 [ 96 ]. In addition, work with our same strain grown at 10 and 150 μmol photons m −2 s −1 obtained F v / F m values of 0.36 and 0.15, respectively [ 97 ].…”

Section: Resultsmentioning

confidence: 99%

Protist impacts on marine cyanovirocell metabolism

Howard-Varona

Roux

Bowen

et al. 2022

ISME Communications

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The fate of oceanic carbon and nutrients depends on interactions between viruses, prokaryotes, and unicellular eukaryotes (protists) in a highly interconnected planktonic food web. To date, few controlled mechanistic studies of these interactions exist, and where they do, they are largely pairwise, focusing either on viral infection (i.e., virocells) or protist predation. Here we studied population-level responses of Synechococcus cyanobacterial virocells (i.e., cyanovirocells) to the protist Oxyrrhis marina using transcriptomics, endo- and exo-metabolomics, photosynthetic efficiency measurements, and microscopy. Protist presence had no measurable impact on Synechococcus transcripts or endometabolites. The cyanovirocells alone had a smaller intracellular transcriptional and metabolic response than cyanovirocells co-cultured with protists, displaying known patterns of virus-mediated metabolic reprogramming while releasing diverse exometabolites during infection. When protists were added, several exometabolites disappeared, suggesting microbial consumption. In addition, the intracellular cyanovirocell impact was largest, with 4.5- and 10-fold more host transcripts and endometabolites, respectively, responding to protists, especially those involved in resource and energy production. Physiologically, photosynthetic efficiency also increased, and together with the transcriptomics and metabolomics findings suggest that cyanovirocell metabolic demand is highest when protists are present. These data illustrate cyanovirocell responses to protist presence that are not yet considered when linking microbial physiology to global-scale biogeochemical processes.

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“…Each protein sample was placed in a quartz cuvette and time-resolved photoluminescence measurements were performed using a time-correlated single-photon counting (TCSPC) technique as described in Kolodny et al 2021. 44 Donor excitation was induced using a Fianium WhiteLase SC-400 supercontinuum pulsed laser, at a repetition rate of 10 MHZ, monochromatized to the donor absorption peak at 462 nm. Collected light was filtered using a short-pass filter, to block the acceptor emission, and a long-pass filter, to block reflected excitation-beam photons.…”

Section: Methodsmentioning

confidence: 99%