Marine Transducing Bacteriophage Attacking a Luminous Bacterium

Keynan, A.; Nealson, Kenneth H.; Sideropoulos, Henry; Hastings, J. Woodland

doi:10.1128/jvi.14.2.333-340.1974

Cited by 26 publications

(19 citation statements)

References 17 publications

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“…Many vibriophages have been described (1,4,(7)(8)(9)(10)(11)(12)16). Most of them are for Vibrio cholerae, some for V. parahaemolyticus, and a few for other Vibrio species.…”

mentioning

confidence: 99%

A Broad‐Host‐Range Vibriophage, KVP40, Isolated from Sea Water

Matsuzaki

Tanaka

Koga

et al. 1992

Microbiology and Immunology

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A broad-host-range vibriophage, KVP40, was isolated from sea water by using Vibrio parahaemolyticus 1010 (EB101) as the indicator host. The host range of KVP40 extended over at least 8 Vibrio and 1 Photobacterium species. KVP40 was a large tailed phage containing double-stranded DNA and belonged to Ackermann's morphotype A2. KVP40 DNA was cleaved by 11 different type II restriction endonucleases including EcoRI and HindIII, but not by 17 other enzymes including BamHI, Kpnl and Sall. Many vibriophages have been described (1,4,(7)(8)(9)(10)(11)(12)16). Most of them are for Vibrio cholerae, some for V. parahaemolyticus, and a few for other Vibrio species. These phages are generally specific for a single Vibrio species on which the individual phage was isolated. This report describes a unique vibriophage isolated from sea water, which showed a relatively broad host specificity for species of family Vibrionaceae. This phage, designated KVP40, belongs to a rather rare morphotype A2 according to Ackermann's classification (1).Bacterial strains, for which the host range of KVP40 was determined, are listed in Table 1. The following culture media were used for experiments. YPH, YP, and YPL media (pH 8.0) contained 0.3% Yeast Extract (Difco Lab., Detroit, Mich., U.S.A.) and 1% Polypepton (Daigo Eiyo-Kagaku, Osaka, Japan) as basal constituents, and were supplemented with 5, 3, and 0.2% NaCl, respectively. Two percent agar was added to the media for solid culture, but 0.5% for soft agar media. Phage KVP40 was isolated from a sample of highly polluted sea water collected at Urado Bay, Kochi, Japan, with V. parahaemolyticus 1010 as the host. Phage lysates (ca. 109 PFU/ml) were prepared by growing strain 1010 with KVP40 in YP medium at 30 C until lysis occurred. Plaque formation of KVP40 on various bacterial species was examined by either the conventional soft-agar-overlay method or the spot test using YP agar medium with the exception that plaque formation on V. costicola was determined with YPH agar. In some experiments, YPL agar was also used. Plates were incubated for 20 hr at 30 C.Host range of phage KVP40 is shown in

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“…Many vibriophages have been described (1,4,(7)(8)(9)(10)(11)(12)16). Most of them are for Vibrio cholerae, some for V. parahaemolyticus, and a few for other Vibrio species.…”

mentioning

confidence: 99%

A Broad‐Host‐Range Vibriophage, KVP40, Isolated from Sea Water

Matsuzaki

Tanaka

Koga

et al. 1992

Microbiology and Immunology

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“…Some were also shown to be psychrophilic [23] The most convincing transduction studies carried out in environmentally relevant experiments were in situ studies in fresh water where transduction of plasmid DNA was shown in Pseudomonas aeruginosa [24]. To our knowledge, no attempts have been made to study transduction directly in marine waters.…”

Section: Transductionmentioning

confidence: 99%

Gene transfer in the marine environment

Hermansson¹

1994

FEMS Microbiology Ecology

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This review summarises the literature on bacterial gene transfer in marine ecosystems. Relevant experiments carried out in model systems are also included. Prerequisites for the main gene transfer mechanisms, transformation, transduction and conjugation are discussed, such as concentrations of extraceUular DNA in marine waters, numbers of bacteriophages in sea water and frequency of plasmids in marine bacteria. Transfer of chromosomal genes as well as plasmids are considered. We also discuss the possibility that gene transfer is more frequent in surface-associated bacterial communities. Examples of relevant studies using various solid surfaces and from the air-water interface are summarised. We suggest that there is a higher 'flow-rate' of genetic information through surface-associated communities compared to bulk water communities.

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“…It was almost three decades ago that the first description of bacteriophages infecting luminescent bacteria was reported (Keynan et al, 1974). After a long gap of 25 years, bacteriophage-mediated toxicity of V. harveyi in Penaeus monodon by the transfer of a gene controlling toxin production was reported (Ruangpan et al, 1999), followed by the description of VHML associated with toxin-producing strains (Oakey & Owens, 2000;Oakey et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

Characterization of four lytic transducing bacteriophages of luminescent Vibrio harveyi isolated from shrimp (Penaeus monodon) hatcheries

Sanjeevi

Bagthasingh

Alavandi

et al. 2011

FEMS Microbiol Lett

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Four lytic bacteriophages designated as φVh1, φVh2, φVh3, and φVh4 were isolated from commercial shrimp hatcheries, possessing broad spectrum of infectivity against luminescent Vibrio harveyi isolates, considering their potential as biocontrol agent of luminescent bacterial disease in shrimp hatcheries, and were characterized by electron microscopy, genomic analysis, restriction enzyme analysis (REA), and pulsed-field gel electrophoresis (PFGE). Three phages φVh1, φVh2, and φVh4 had an icosahedral head of 60-115 nm size with a long, noncontractile tail of 130-329 × 1-17 nm, belonged to the family Siphoviridae. φVh3 had an icosahedral head (72 ± 5 nm) with a short tail (27 × 12 nm) and belonged to Podoviridae. REA with DraI and PFGE of genomic DNA digested with ScaI and XbaI and cluster analysis of their banding patterns indicated that φVh3 was distinct from the other three siphophages. PFGE-based genome mean size of the four bacteriophages φVh1, φVh2, φVh3, and φVh4 was estimated to be about 85, 58, 64, and 107 kb, respectively. These phages had the property of generalized transduction as demonstrated by transduction with plasmid pHSG 396 with frequencies ranging from 4.1 × 10(-7) to 2 × 10(-9) per plaque-forming unit, suggesting a potential ecological role in gene transfer among aquatic vibrios.

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Marine Transducing Bacteriophage Attacking a Luminous Bacterium

Cited by 26 publications

References 17 publications

A Broad‐Host‐Range Vibriophage, KVP40, Isolated from Sea Water

A Broad‐Host‐Range Vibriophage, KVP40, Isolated from Sea Water

Gene transfer in the marine environment

Characterization of four lytic transducing bacteriophages of luminescent Vibrio harveyi isolated from shrimp (Penaeus monodon) hatcheries

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