Marker-assisted pyramiding of resistance loci to grape downy mildew

Saifert, Luciano; Sánchez-Mora, Fernando David; Assumpção, Wilson Taybar; Zanghelini, Jean Alberto; Giacometti, Renan; Novak, Eduardo Irineu; Vesco, Lírio Luiz Dal; Nodari, Rubens Onofre; Eibach, Rudolf; Welter, Leocir José

doi:10.1590/s0100-204x2018000500009

Cited by 25 publications

(20 citation statements)

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“…Further experiments with genotypes containing Rpv3–2, in the absence of Rpv3–1, are required to determine whether the Rpv3–2 -mediated resistance is also compromised by this avrRpv3ˉ isolate. The combination (pyramiding) of different R loci is recognized as an important strategy to increase durability of resistance against plant pathogens [2, 58]. An understanding of the mechanisms underlying R gene mediated resistance and the recognized avr effectors will be crucial role in finding successful resistance loci combinations to guarantee a durable resistance against grapevine downy mildew.…”

Section: Discussionmentioning

confidence: 99%

Rpv3–1 mediated resistance to grapevine downy mildew is associated with specific host transcriptional responses and the accumulation of stilbenes

et al. 2019

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Background European grapevine cultivars ( Vitis vinifera spp.) are highly susceptible to the downy mildew pathogen Plasmopara viticola . Breeding of resistant V. vinifera cultivars is a promising strategy to reduce the impact of disease management. Most cultivars that have been bred for resistance to downy mildew, rely on resistance mediated by the Rpv3 ( R esistance to P . v iticola ) locus. However, despite the extensive use of this locus, little is known about the mechanism of Rpv3 -mediated resistance. Results In this study, Rpv3 -mediated defense responses were investigated in Rpv3 + and Rpv3ˉ grapevine cultivars following inoculation with two distinct P. viticola isolates avrRpv3 + and avrRpv3ˉ , with the latter being able to overcome Rpv3 resistance. Based on comparative microscopic, metabolomic and transcriptomic analyses, our results show that the Rpv3–1 -mediated resistance is associated with a defense mechanism that triggers synthesis of fungi-toxic stilbenes and programmed cell death (PCD), resulting in reduced but not suppressed pathogen growth and development. Functional annotation of the encoded protein sequence of genes significantly upregulated during the Rpv3–1 -mediated defense response revealed putative roles in pathogen recognition, signal transduction and defense responses. Conclusion This study used histochemical, transcriptomic and metabolomic analyses of Rpv3 + and susceptible cultivars inoculated with avirulent and virulent P. viticola isolates to investigate mechanism underlying the Rpv3–1 -mediated resistance response. We demonstrated a strong correlation between the expressions of stilbene biosynthesis related genes, the accumulation of fungi-toxic stilbenes, pathogen growth inhibition and PCD. Electronic supplementary material The online version of this article (10.1186/s12870-019-1935-3) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Rpv3–1 mediated resistance to grapevine downy mildew is associated with specific host transcriptional responses and the accumulation of stilbenes

et al. 2019

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“…SSRs are highly polymorphic and transferable among several species of the genus Vitis [17]. Since SSRs provide unique fingerprints for cultivar identification [18], they have been used for genetic resource characterization [19,20], parentage analysis [21,22], genetic mapping [23,24], detection of quantitative trait loci (QTLs) [25], and assisted selection [26].…”

Section: Plos Onementioning

confidence: 99%

Genetic structure and molecular diversity of Brazilian grapevine germplasm: Management and use in breeding programs

Oliveira¹,

Souza

Oliveira

et al. 2020

PLoS ONE

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The management of germplasm banks is complex, especially when many accessions are involved. Microsatellite markers are an efficient tool for assessing the genetic diversity of germplasm collections, optimizing their use in breeding programs. This study genetically characterizes a large collection of 410 grapevine accessions maintained at the Agronomic Institute of Campinas (IAC) (Brazil). The accessions were genotyped with 17 highly polymorphic microsatellite markers. Genetic data were analyzed to determine the genetic structure of the germplasm, quantify its allelic diversity, suggest the composition of a core collection, and discover cases of synonymy, duplication, and misnaming. A total of 304 alleles were obtained, and 334 unique genotypes were identified. The molecular profiles of 145 accessions were confirmed according to the literature and databases, and the molecular profiles of more than 100 genotypes were reported for the first time. The analysis of the genetic structure revealed different levels of stratification. The primary division was between accessions related to Vitis vinifera and V. labrusca, followed by their separation from wild grapevine. A core collection of 120 genotypes captured 100% of all detected alleles. The accessions selected for the core collection may be used in future phenotyping efforts, in genome association studies, and for conservation purposes. Genetic divergence among accessions has practical applications in grape breeding programs, as the choice of relatively divergent parents will maximize the frequency of progeny with superior characteristics. Together, our results can enhance the management of grapevine germplasm and guide the efficient exploitation of genetic diversity to facilitate the development of new grape cultivars for fresh fruits, wine, and rootstock.

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“…Similarly, in apple, pyramiding of three resistance QTLs to scab resulted in durable resistance which acted at different stages of fungal infection cycle ( Laloi et al, 2017 ). Marker-assisted gene pyramiding by combining two or more downy mildew resistance genes was performed in several crops such as in grape vine ( Schwander et al, 2012 ; Nascimento-Gavioli et al, 2017 ; Saifert et al, 2018 ) and sunflower ( Qi et al, 2017 ; Qi and Ma, 2019 ). Pyramiding of QTLs with different specificities/broad-spectrum QTLs, and QTLs associated with different resistance mechanism are expected to increase the durability by showing stable levels of resistance in multiple environments.…”

Section: Downy Mildewmentioning

confidence: 99%

Molecular Breeding Strategy and Challenges Towards Improvement of Downy Mildew Resistance in Cauliflower (Brassica oleracea var. botrytis L.)

et al. 2021

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Cauliflower (Brassica oleracea var. botrytis L.) is one of the important, nutritious and healthy vegetable crops grown and consumed worldwide. But its production is constrained by several destructive fungal diseases and most importantly, downy mildew leading to severe yield and quality losses. For sustainable cauliflower production, developing resistant varieties/hybrids with durable resistance against broad-spectrum of pathogens is the best strategy for a long term and reliable solution. Identification of novel resistant resources, knowledge of the genetics of resistance, mapping and cloning of resistance QTLs and identification of candidate genes would facilitate molecular breeding for disease resistance in cauliflower. Advent of next-generation sequencing technologies (NGS) and publishing of draft genome sequence of cauliflower has opened the flood gate for new possibilities to develop enormous amount of genomic resources leading to mapping and cloning of resistance QTLs. In cauliflower, several molecular breeding approaches such as QTL mapping, marker-assisted backcrossing, gene pyramiding have been carried out to develop new resistant cultivars. Marker-assisted selection (MAS) would be beneficial in improving the precision in the selection of improved cultivars against multiple pathogens. This comprehensive review emphasizes the fascinating recent advances made in the application of molecular breeding approach for resistance against an important pathogen; Downy Mildew (Hyaloperonospora parasitica) affecting cauliflower and Brassica oleracea crops and highlights the QTLs identified imparting resistance against this pathogen. We have also emphasized the critical research areas as future perspectives to bridge the gap between availability of genomic resources and its utility in identifying resistance genes/QTLs to breed downy mildew resistant cultivars. Additionally, we have also discussed the challenges and the way forward to realize the full potential of molecular breeding for downy mildew resistance by integrating marker technology with conventional breeding in the post-genomics era. All this information will undoubtedly provide new insights to the researchers in formulating future breeding strategies in cauliflower to develop durable resistant cultivars against the major pathogens in general and downy mildew in particular.

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Marker-assisted pyramiding of resistance loci to grape downy mildew

Cited by 25 publications

References 20 publications

Rpv3–1 mediated resistance to grapevine downy mildew is associated with specific host transcriptional responses and the accumulation of stilbenes

Rpv3–1 mediated resistance to grapevine downy mildew is associated with specific host transcriptional responses and the accumulation of stilbenes

Genetic structure and molecular diversity of Brazilian grapevine germplasm: Management and use in breeding programs

Molecular Breeding Strategy and Challenges Towards Improvement of Downy Mildew Resistance in Cauliflower (Brassica oleracea var. botrytis L.)

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