Markers of bile duct tumors

Malaguarnera, Giulia; Giordano, Maria; Paladina, Isabella; Rando, Alessandra; Uccello, Mario; Burzotta, Francesco; Biondi, Antonio; Carnazzo, Santo; Alessandria, I.; Mazzarino, C

doi:10.4251/wjgo.v3.i4.49

Cited by 35 publications

(22 citation statements)

References 142 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Mucins are glycosylated proteins that are normally expressed in the ductal and glandular epithelium of epithelial tissues [17]. The use of MUC 5 Ac (serum mucin) has been studied as a marker for bile duct cancer and as a diagnostic method and, in some cases, as a prognostic evaluation [18,19].…”

Section: Discussionmentioning

confidence: 99%

Absorbable bioprosthesis for the treatment of bile duct injury in an experimental model

Montalvo-Javé

Barrera

Treviño

et al. 2015

International Journal of Surgery

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 99%

Absorbable bioprosthesis for the treatment of bile duct injury in an experimental model

Montalvo-Javé

Barrera

Treviño

et al. 2015

International Journal of Surgery

View full text Add to dashboard Cite

show abstract

“…Markers such as p53, bcl-2, bax, S100A4, mesothelin, COX-2, P-cadherin, Dpc4/ SMAD4, topoisomerase IIa, cdc2/p34, fascin, 14-3-3s, and MUC mucins are among those that have been previously tested to show variable sensitivity and specificity. [6][7][8][9][10][11][12][13][14] Our recent study has shown that combined use of the insulin-like growth factor-II mRNA binding protein-3 (IMP3), S100P, and the von Hippel-Lindau gene product (pVHL) as an immunohistochemical panel is valuable in the detection of adenocarcinoma in bile duct biopsies. 15 By examining 72 histologically well-characterized endoscopic bile duct biopsies (40 malignant, 32 benign/reactive), we found that bile duct adenocarcinoma frequently showed an IMP3-positive/S100P-positive/pVHL-negative staining pattern, followed by an IMP3-negative/S100P-positive/ pVHL-negative pattern.…”

mentioning

confidence: 99%

Use of IMP3, S100P, and pVHL Immunopanel to Aid in the Interpretation of Bile Duct Biopsies With Atypical Histology or Suspicious for Malignancy

Schmidt

Himmelfarb

Shafi

et al. 2012

Applied Immunohistochemistry &Amp; Molecular Morphology

View full text Add to dashboard Cite

Histologic evaluation of an endoscopic bile duct biopsy for malignancy is a known challenge. Our prior study has shown that the insulin-like growth factor-II mRNA binding protein-3 (IMP3), S100P, and the von Hippel-Lindau gene product (pVHL) are a useful immunopanel for the distinction between adenocarcinoma and benign biliary epithelium. To further evaluate the usefulness of the IMP3, S100P, and pVHL immunopanel to aid in the interpretation of bile duct biopsies, 16 histologically challenging bile duct biopsies that exhibited atypical histology or features suspicious for malignancy were immunohistochemically stained for IMP3, S100P, and pVHL. Clinical follow-up data were obtained for each case. The results showed that in the 11 cases that showed adenocarcinoma during follow-up, the following staining patterns in atypical/suspicious cells in the initial biopsies were observed: IMP3-positive/S100P-positive/pVHL-negative or reduced (n=6), IMP3-negative/S100P-positive/pVHL-negative or reduced (n=4), and IMP3-positive/S100P-negative/pVHL-negative (n=1). In the 5 follow-up-proven benign cases, 2 biopsies showed an IMP3-negative/S100P-positive/pVHL-positive pattern in atypical cells and 1 was negative for all 3 proteins. The remaining 2 biopsies exhibited an IMP3-positive/S100P-positive/pVHL-negative or reduced pattern in atypical cells that were histologically considered dysplastic on retrospective review. These observations reaffirm that bile duct adenocarcinoma frequently shows positive IMP3 and/or S100P staining with reciprocal negative or reduced pVHL expression. This staining pattern can also be seen in dysplastic epithelium in the absence of invasive carcinoma. On the contrary, benign biliary epithelium typically lacks IMP3 immunoreactivity and may retain normal pVHL expression. However, caution should be exercised when using this immunopanel in the interpretation of challenging bile duct biopsies, because S100P and pVHL stains may give rise to variable patterns that can be difficult to interpret.

show abstract

“…CEA, a highly glycosylated cell surface glycoprotein, is considered a colon-specific oncofetal protein 5 and is often found in patients with malignant tumors of the digestive system such as stomach, colon, biliary tract, and pancreas cancer. 11 In the current case, neoplastic cells were negative for CEA, whereas, in human medicine, this marker shows a high sensitivity and specificity for cholangiocarcinoma. 13,14 As the inner control was also negative for both CEA and TTF1, it was concluded that these human antibodies do not react with llama biliary and pulmonary epithelium.…”

Section: Panopticmentioning

confidence: 98%

Metastatic cholangiocarcinoma in a llama (Lama glama)

Taulescu

Bolfă²,

Buiga³

et al. 2012

J VET Diagn Invest

View full text Add to dashboard Cite

A 2-year-old female llama (Lama glama) from a private zoological park was submitted to the Pathology Department of the Faculty of Veterinary Medicine from Cluj-Napoca (Romania) for necropsy. The animal had a history of depression, anorexia, progressive weakening, ataxia, decubitus ulcers, fever, dyspnea, and abdominal distension. Death occurred 6 weeks after the onset of clinical signs. Necropsy, and cytological, histological, and immunohistochemical examinations were performed. Grossly, the animal was emaciated. Subcutaneous edema of the posterior limbs, abdomen, and thorax, hydrothorax, hydro-pericardium, and severe ascites (approximately 15 liters of fluid) with fibrin clots adherent to the visceral peritoneum, and multiple, acute, gastric ulcers were observed.A firm, white-gray, multinodular mass, 25 cm in diameter was found in the liver parenchyma. Moreover, multiple small nodules, unencapsulated, with poorly defined margins, and ranging from 0.5 to 4 cm in diameter scattered throughout the liver parenchyma were present. The cut surface of the tumors varied from gray-white to red-brown (Fig. 1). Multiple areas of necrosis, a few microabscesses, and venous thrombosis were also found within the liver parenchyma. Similar nodular lesions affecting the mesenteric, tracheobronchial, and submandibular lymph nodes, lungs (Fig. 2), parietal pleura, pericardium, diaphragm, the serosal surface of the gut, and peritoneum were present. Lesions were umbelicated, firm, and gray, and ranged from 0.5 to 3 cm in diameter. The lymph nodes were markedly enlarged and multifocally replaced by metastatic lesions. No tumors were detected in other organs, including kidney, stomach, pancreas, and mammary glands.Samples from liver, lung, lymph nodes, diaphragm, and pericardium were evaluated by cytological, histological, and immunohistochemical examinations. For cytology, multiple smears from the hepatic masses were stained by Dia-Quick Panoptic.a For histology, the samples were fixed in 10% phosphate buffered formalin for 24 hr, embedded in paraffin wax, cut into 3-5 µm sections, and stained with hematoxylin and eosin and periodic acid-Schiff (PAS). Histological interpretation of lesions was done according to the World Health Organization Classification of Tumors of the Alimentary System of Domestic Animals.6 For immunohistochemistry, sections from liver mass, lung nodules, and normal liver and lung tissue (inner control) were selected. After dewaxing, heat-induced epitope retrieval pretreatment, and endogenous peroxidase inactivation (H 2 O 2 1% in phosphate buffered saline b [PBS]), slides were incubated with goat serum 10% in PBS for reducing background staining. Sections were incubated with the following mouse monoclonal primary antibodies: broad spectrum cytokeratins (panCK), c CK20, c CK19, c CK7, c thyroid transcription factor 1 (TTF1), c and carcinoembryonic antigen (CEA), b for 24 hr at 4°C. After washes, the secondary antibody (labeled streptavidin biotin) was applied, followed by incubation with diaminobenzidine. Negative...

show abstract

Markers of bile duct tumors

Cited by 35 publications

References 142 publications

Absorbable bioprosthesis for the treatment of bile duct injury in an experimental model

Absorbable bioprosthesis for the treatment of bile duct injury in an experimental model

Use of IMP3, S100P, and pVHL Immunopanel to Aid in the Interpretation of Bile Duct Biopsies With Atypical Histology or Suspicious for Malignancy

Metastatic cholangiocarcinoma in a llama (Lama glama)

Contact Info

Product

Resources

About