Mass Mortalities of Juvenile Abalones, Haliotis spp., Caused by Amyotrophia.

Momoyama, Kazuo; Nakatsugawa, Toshio; Yurano, Noriyoshi

doi:10.3147/jsfp.34.7

Cited by 15 publications

(22 citation statements)

References 4 publications

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“…As an example, Nakatsugawa et al [30] identified the virus in a primary culture of hemocytes of Japanese black abalone Nordotic discus discus which was affected with amyotrophia. Later, the amyotrophia infection was identified in H. discus hannai [31], H. discus discus, Haliotis madeka species [32]. Moreover, herpes-like virus has been isolated from ganglioneuritis affected abalone [33].…”

Section: Discussionmentioning

confidence: 99%

Allograft inflammatory factor-1 in disk abalone (Haliotis discus discus): Molecular cloning, transcriptional regulation against immune challenge and tissue injury

Zoysa

Nikapitiya

Kim

et al. 2010

Fish & Shellfish Immunology

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Section: Discussionmentioning

confidence: 99%

Allograft inflammatory factor-1 in disk abalone (Haliotis discus discus): Molecular cloning, transcriptional regulation against immune challenge and tissue injury

Zoysa

Nikapitiya

Kim

et al. 2010

Fish & Shellfish Immunology

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“…The present study has important implications for the conservation of the abalone resource. Techniques for artificial production of Ezo‐awabi seedlings are well established, but the production of Kuro‐awabi seedlings has been hampered, due in large part to the high mortality of seedlings caused by infectious disease 29 . A growing concern underlies whether the mass‐release of Ezo‐awabi seedlings into the habitat of Kuro‐awabi so as to increase the abalone resource is acceptable in the sense of the ecology, biology and genetics of the resource.…”

Section: Discussionmentioning

confidence: 99%

Genetic difference between Ezo-awabi Haliotis discus hannai and Kuro-awabi H. discus discus populations: Microsatellite-based population analysis in Japanese abalone

Hara

Sekino

2005

Fisheries Sci

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This study documents the genetic population relationships between Ezo-awabi Haliotis discus hannai and Kuro-awabi H. discus discus by means of the microsatellite technique with an emphasis on the extent of genetic difference between Ezo-and Kuro-awabi populations. Eight markers were employed to screen five populations each collected from Ezo-and Kuro-awabi habitats. All eight loci showed polymorphisms in all populations (number of alleles per locus, 11.1-14.1; averaged expected heterozygosity, 0.64-0.70). The number of loci that yielded significant genetic heterogeneities (allele frequency distribution and/or pairwise q ) between Ezo-and Kuro-awabi populations was larger than that generated in comparisons between populations within Ezo-and Kuro-awabi habitats. According to the bootstrap neighbor-joining trees constructed on the basis of two genetic distance measures ( D A and D ST ), the 10 populations fell into two clusters of populations (Ezo-awabi and Kuro-awabi habitat groups), and the results of hierarchical AMOVA testing also supported the neighborjoining clustering. The outcomes presented here suggest that the microsatellite markers used in this study are potentially an efficient means to differentiate Ezo-and Kuro-awabi populations.

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“…Virus-like particles with a diameter of 50-60 nm were observed in secondary lysosomes in the cytoplasm of cells of affected abalone 9 . These authors described that these particles were not likely to be the etiological agent because the size of the causative agent was estimated to be between 100-220 nm by filtration experiments 9,16 .…”

mentioning

confidence: 99%

A novel Asfarvirus-like virus identified as a potential cause of mass mortality of abalone

Matsuyama

Takano

Nishiki

et al. 2020

Sci Rep

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A novel Asfarvirus-like virus is proposed as the etiological agent responsible for mass mortality in abalone. The disease, called abalone amyotrophia, originally was recognized in the 1980s, but efforts to identify a causative agent were unsuccessful. We prepared a semi-purified fraction by nuclease treatment and ultracentrifugation of diseased abalone homogenate, and the existence of the etiological agent in the fraction was confirmed by a challenge test. Using next-generation sequencing and PCR-based epidemiological surveys, we obtained a partial sequence with similarity to a member of the family Asfarviridae. BLASTP analysis of the predicted proteins against a virus database resulted in 48 proteins encoded by the novel virus with top hits against proteins encoded by African swine fever virus (ASFV). Phylogenetic analyses of predicted proteins of the novel virus confirmed that ASFV represents the closest relative. Comparative genomic analysis revealed gene-order conservation between the novel virus and ASfV. In situ hybridization targeting the gene encoding the major capsid protein of the novel virus detected positive signals only in tissue from diseased abalone. The results of this study suggest that the putative causative agent should be considered a tentative new member of the family Asfarviridae, which we provisionally designate abalone asfa-like virus (AbALV). African swine fever virus (ASFV) is the causative agent of African swine fever (ASF). The virus causes a hemorrhagic fever with high mortality, with rates approaching 100% in domestic pigs 1. The virus infects domestic pigs and their relatives and ticks 2. ASF outbreaks had been recorded in Africa and Europe, but in recent years the disease has spread to China, Vietnam, Cambodia, Mongolia, Hong Kong, and Korea, becoming a threat to the swine industry worldwide 3. ASFV is a member of nucleocytoplasmic large DNA viruses (NCLDVs) with an average diameter of 200 nm. Although some related viruses, such as faustovirus 4 , kaumoebavirus 5 , and pacmanvirus 6 , have been reported, ASFV is the only member of the Asfarviridae family 7. In the present paper, we describe a virus likely to be the closest ASFV relative found to date; this novel virus was isolated as the presumptive causative agent of abalone amyotrophia. Mass mortalities of abalone have been reported since the early 1980s, during seed production in Japan. The disease was designated abalone amyotrophia because diseased abalone develop muscle atrophy in the mantle and foot 8. Diseased abalone show reduced ability to adhere to the substrate, and some diseased abalone exhibit incisions on the front margin of the shell and brown pigmentation inside of the shell 9. Histopathological evaluation has revealed the presence of abnormal cell masses that are produced extensively, primarily in the ganglion and peripheral nerve of the foot muscle 9. Cumulative mortality can reach 50% and higher 10. Abalone herpesvirus (AbHV) 11,12 and abalone shriveling syndrome-associated virus (AbSV) 13 also cause mortality ac...

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Mass Mortalities of Juvenile Abalones, Haliotis spp., Caused by Amyotrophia.

Cited by 15 publications

References 4 publications

Allograft inflammatory factor-1 in disk abalone (Haliotis discus discus): Molecular cloning, transcriptional regulation against immune challenge and tissue injury

Allograft inflammatory factor-1 in disk abalone (Haliotis discus discus): Molecular cloning, transcriptional regulation against immune challenge and tissue injury

Genetic difference between Ezo-awabi Haliotis discus hannai and Kuro-awabi H. discus discus populations: Microsatellite-based population analysis in Japanese abalone

A novel Asfarvirus-like virus identified as a potential cause of mass mortality of abalone

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