Mass Propagation of Rauwolfia serpentina L. Benth

Abstract

A protocol for mass propagation through axillary bud proliferation was established for Rauwolfia serpentina L. Benth. (Apocynaceae). MS medium supplemented with 1.5 mg L(-1) BA and 0.2 mg L(-1) NAA elicited the maximum number of shoots (4 multiple shoots) from nodal explants. These adventitious shoots were best rooted on half strength MS medium supplemented with 1.0 mg L(-1) each of IBA and IAA. The in vitro raised plants were acclimatized in glass house and successfully transplanted to field condition with almost 95% survival.

Improvedin vitroclonal propagation ofRauwolfia serpentinaL. Benth – An endangered medicinal plant

“…The highest percentage of shoot bud proliferation (96%) and the maximum rate of shoot multiplication (13.3 shoots/explants) with a maximum shoot length of 8.2 cm was obtained in MS medium supplemented with 2 mg/l BAP þ 0.25 mg/l NAA þ 3% sucrose (Table I). In contrast to our results, Salma et al (2008) reported 90% shoot formation with 3.89 shoots/explants and an average shoot length of 4.08 cm in MS þ 1.5 mg/l BAP þ 0.2 mg/l NAA, whereas Jain et al (2003) reported 85.6% of shoot initiation in MS þ 5.0 mg/l BAP þ 0.5 mg/l IBA. Similarly, Mallick et al (2012) observed maximum shoot multiplication (3.89/ explant) and shoot growth (mean shoot length 4.08 cm) on MS media supplemented with 1.0 mg/l BAP þ 0.1 mg/l NAA.…”

“…The plant growth regulator (PGR)-free MS medium failed to induce bud proliferation, whereas the MS medium supplemented with PGR enhanced the rate of shoot bud proliferation as reported by Salma et al (2008). The highest percentage of shoot bud proliferation (96%) and the maximum rate of shoot multiplication (13.3 shoots/explants) with a maximum shoot length of 8.2 cm was obtained in MS medium supplemented with 2 mg/l BAP þ 0.25 mg/l NAA þ 3% sucrose (Table I).…”

In vitro regeneration protocol of Rauvolfia serpentina L.

“…In case of leaf explants maximum shoots (9.1) were obtained on MS 2.0 mg/l BAP and 0.5 mg/l NAA supplemented media. Panwar et al (2011) observed that maximum shoots of R. serpentina (25.4 per culture) were obtained from the callus inoculated in shooting medium containing BAP 2.0 mg/l + NAA 0.5 mg/ l. MS medium supplemented with 1.5 mg/l BA and 0.2 mg/l NAA elicited the maximum number of shoots (4 multiple shoots) from nodal explants (Salma et al, 2008). Susila et al (2013) observed best shoot proliferation (92%) in MS medium containing 0.1 mg /l NAA and 2.5 mg/l BA.…”

Effect of copper and zinc on the in vitro regeneration of Rauvolfia serpentina

“…Also Rauvolfia serpentina is seriously threatened with extinction, and propagation through seeds or vegetative parts cannot meet the increasing demand. Therefore, some alternatives, such as plant tissue culture have been adopted (Mathur et al 1987, Mukhopadhyay et al 1991, Roy et al, 1995, Roja and Heble 1996, Tiwari et al 2003, Kataria and Shekhawat 2005, Baksha et al 2007, Bhatt et al 2008, Salma et al 2008, Mishra et al 2010. Surprisingly, these available reports are based strictly on the optimization of different plant growth regulators (PGRs), especially cytokinins.…”