Mass spectrometry imaging: An emerging technology for the analysis of metabolites in insects

Yang, Fei-Ying; Chen, Junhui; Ruan, Qian-Qian; Saqib, Hafiz Sohaib Ahmed; He, Weiyi; You, Minsheng

doi:10.1002/arch.21643

Cited by 19 publications

(19 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Due to these limitations, the use of SIMS in food science is limited to the characterization of small molecule compounds such as elements and lipids, but has the advantage of a very high lateral resolution (>1 µm) compared with other ionization techniques (Table 1). The second ionization technique used for MSI is MALDI, which has been widely used in not only food science [11,12], but also in many biological research fields [18][19][20][21]. The first report on the utilization of MALDI-MS was by Karas and Hillenkamp in 1988, who performed successful ionization of serum At the start of MSI history in 1962, SIMS revealed the first ion images, which showed the distribution of Mg + , Al + , and Si + ions in the specimen [16].…”

Section: History Of Msi and Widely Used Ionization Methodsmentioning

confidence: 99%

Application of Mass Spectrometry Imaging for Visualizing Food Components

Yoshimura

Zaima

2020

Foods

View full text Add to dashboard Cite

Consuming food is essential for survival, maintaining health, and triggering positive emotions like pleasure. One of the factors that drive us toward such behavior is the presence of various compounds in foods. There are many methods to analyze these molecules in foods; however, it is difficult to analyze the spatial distribution of these compounds using conventional techniques, such as mass spectrometry combined with high-performance liquid chromatography or gas chromatography. Mass spectrometry imaging (MSI) is a two-dimensional ionization technology that enables detection of compounds in tissue sections without extraction, purification, separation, or labeling. There are many methods for ionization of analytes, including secondary ion mass spectrometry, matrix-assisted laser desorption/ionization, and desorption electrospray ionization. Such MSI technologies can provide spatial information on the location of a specific analyte in food. The number of studies utilizing MSI technologies in food science has been increasing in the past decade. This review provides an overview of some of the recent applications of MSI in food science and related fields. In the future, MSI will become one of the most promising technologies for visualizing the distribution of food components and for identifying food-related factors by their molecular weights to improve quality, quality assurance, food safety, nutritional analysis, and to locate administered food factors.

show abstract

Section: History Of Msi and Widely Used Ionization Methodsmentioning

confidence: 99%

Application of Mass Spectrometry Imaging for Visualizing Food Components

Yoshimura

Zaima

2020

Foods

View full text Add to dashboard Cite

show abstract

“…The MALDI-MSI [60][61][62] was conducted in larvae of two contrasting phenotypes (FS and FT, Figure 5a) and also on FT-larvae that were slowly pre-frozen to -30°C (FT-frozen, Fig. 5b).…”

Section: Matrix-assisted Laser Desorption/ionization Mass Spectrometry Imaging (Maldi-msi)mentioning

confidence: 99%

“…The MALDI-MSI (Khalil et al, 2017;Tuthill II et al, 2020;Yang et al, 2020) was conducted in LD and SDA larvae and also on SDA-frozen larvae (Fig. A1).…”

Section: Matrix-assisted Laser Desorption/ionization Mass Spectrometry Imaging (Maldi-msi)mentioning

confidence: 99%

A mixture of innate cryoprotectants is key for freeze tolerance and cryopreservation of a drosophilid fly larva

Kučera

Moos

Štětina

et al. 2021

Preprint

View full text Add to dashboard Cite

BackgroundOrganisms evolved biochemical strategies to cope with environmental stressors. For instance, insects that naturally tolerate internal freezing produce complex mixtures of multiple cryoprotectants (CPs). Better knowledge on composition of these mixtures, and on mechanisms of how the individual CPs interact, could inspire development of laboratory CP formulations optimized for cryopreservation of cells and other biological material.ResultsHere we identify and quantify (using high resolution mass spectrometry) a range of putative CPs in larval tissues of a subarctic fly, Chymomyza costata, that survives long-term cryopreservation in liquid nitrogen. The CPs (proline, trehalose, glutamine, asparagine, glycine betaine, glycerophosphoethanolamine, glycerophosphocholine, and sarcosine) accumulate in hemolymph in a ratio of 313:108:55:26:6:4:3:0.5 mmol.L-1. Using calorimetry, we show that the artificial mixtures, mimicking the concentrations of major CPs’ in hemolymph of freeze-tolerant larvae, suppress the melting point of water and significantly reduce the ice fraction. We demonstrate in a bioassay that mixtures of CPs administered through the diet act synergistically rather than additively to enable cryopreservation of otherwise freeze-sensitive larvae. Using MALDI-MSI, we show that during slow extracellular freezing of whole larvae trehalose becomes concentrated in partially dehydrated hemolymph and stimulates transition to the amorphous glass phase. In contrast, proline moves to the boundary between extracellular ice and dehydrated hemolymph and tissues where it likely forms a layer of dense viscoelastic liquid.ConclusionOur results suggest that different components of innate cryoprotective mixtures of freeze-tolerant insect act in synergy during extracellular freezing. We propose that transitions to amorphous glass (stimulated by trehalose) and viscoelastic liquids (having proline as major component) may protect macromolecules and cells from thermomechanical shocks associated with freezing and transfer into and out of liquid nitrogen.

show abstract

“…Mass spectrometry imaging (MSI) is a powerful technique used for visualising spatial distribution of molecules in situ without prior labelling. It allows for performing the analysis from tissues to cells without any a priori knowledge of the potential target (Yang et al, 2020). MSI has been used in various studies of compounds, such as lipids, proteins and peptides.…”

Section: Introductionmentioning

confidence: 99%

Recent developments of MALDI MSI application in plant tissues analysis

et al. 2020

View full text Add to dashboard Cite

Mass spectrometry imaging (MSI) combined with matrix-assisted laser desorption/ionization (MALDI) is an efficient technology applied in plant metabolomics research. This technique allows for visualization of spatial distribution of metabolites such as: lipids, proteins, peptides and DNA sequences, by determining the x, y coordinates of the compounds exactly in plant tissue. Simplicity of the tissue preparation without the need of prior exact knowledge about the analytes is a great advantage of this method. In this review, we provide an overview of experimental workflow including sample preparation, data acquisition and analysis, methodology, and some recent applications of MALDI MS imaging in plant metabolomics research.

show abstract

Mass spectrometry imaging: An emerging technology for the analysis of metabolites in insects

Cited by 19 publications

References 55 publications

Application of Mass Spectrometry Imaging for Visualizing Food Components

Application of Mass Spectrometry Imaging for Visualizing Food Components

A mixture of innate cryoprotectants is key for freeze tolerance and cryopreservation of a drosophilid fly larva

Recent developments of MALDI MSI application in plant tissues analysis

Contact Info

Product

Resources

About