Maternal supplementation with corn oil associated or not with di-n-butyl phthalate increases circulating estradiol levels of gerbil offspring and impairs sperm reserve

Abstract

This study evaluated the consequences of gestational exposure to di-n-butyl phthalate (DBP) for testicular steroidogenesis and sperm parameters of the adult gerbil and the interference of corn oil (co), a vehicle widely used for administration of liposoluble agents, on DBP effects. Pregnant gerbils received no treatment or were treated from gestational day 8 to 23 via gavage with 0.1 mL/day of co only or containing DBP (100 mg/kg/day). Maternal co intake enhanced serum estradiol levels and testicular content of ERα, and reduced sperm reserve of adult offspring. Gestational DBP exposure caused dyslipidemia, increased serum and intratesticular estradiol levels and reduced sperm reserve and motility. Thus, maternal co supplementation alters circulating estradiol and impairs sperm quantity and quality of offspring. Gestational DBP exposure alters lipid metabolism and testicular steroidogenesis and worsens the negative effects of co on the sperm reserve and motility of gerbil. Therefore, co interferes with the reproductive response to DBP.

Exposure to Dibutyl Phthalate and Reproductive-Related Outcomes in Animal Models: Evidence From Rodents Study

“…Through the evaluation of the rest of the researches, nine researches, including conference reports, non-human studies, or studies unrelated to the analysis, were excluded. In the end, we included 19 researches for analysis ( Tsutsumi et al, 2004 ; Zhang et al, 2004 ; Farombi et al, 2007 ; Hutchison et al, 2008 ; Kim et al, 2010 ; Scarano et al, 2010 ; Bao et al, 2011 ; Ahmad et al, 2014 ; Giribabu et al, 2014 ; Nair, 2015 ; Aly et al, 2016 ; Dobrzyńska et al, 2017 ; Giribabu and Reddy, 2017 ; Nelli and Pamanji, 2017 ; Negrin et al, 2018 ; de Souza et al, 2019 ; Venturelli et al, 2019 ; Wang et al, 2019 ) ( Table 2 ).…”

Postnatal exposure to finasteride causes different effects on the prostate of male and female gerbils

“…In addition to exogenous hormone exposure, other substances, which are not hormones but which alter normal hormonal function—called endocrine disruptors—, also have the capacity of altering prostate development (Doshi et al, 2011; Vandenberg et al, 2011; Sanches et al, 2016b). These substances, such as BPA (Josephson, 2006; Colleta et al, 2017), phthalates (Guo and Kannan, 2013; Christante et al, 2018; Negrin et al, 2018) and parabens (Boberg et al, 2010; Costa et al, 2017), mimic the action of hormones on the body. Other compounds act on enzymes that metabolize steroids, including anastrozole, exemestane and letrozole; these have an inhibitory role on aromatase, an enzyme that converts testosterone to oestradiol (Ellem and Risbridger, 2010).…”

Efeitos do excesso de lipídios saturados e da curcumina na dieta materna sobre os parâmetros reprodutivos de ratos machos adultos

“…Portanto, é possível que os fitoesteroides desse óleo possam ter levado à programação do metabolismo lipídico da prole, em particular no fígado. De fato, esse não é um achado ocasional, pois outros estudos do nosso laboratório mostraram que a suplementação diária de gerbilos do 8º ao 23º dia gestacional com óleo de milho também afetou o metabolismo lipídico da prole [65]. Essa programação do desenvolvimento pelos fitoesteroides do óleo de milho pode ter ocorrido via modulação da expressão de receptores nucleares envolvidos com o metabolismo lipídico e que são ligantes de esteroides, como os LXRs [66].…”

“…Conforme citado anteriormente, o óleo de milho na dieta materna também alterou o metabolismo lipídico e reduziu a reserva espermática dos gerbilos da Mongólia e prejudicou a quantidade e a qualidade dos espermatozoides da prole adulta [65]. Então, os prejuízos reprodutivos da suplementação materna com óleo de milho na gestação/lactação parecem ser comuns aos roedores, embora no gerbilo reduzindo reserva espermática e no rato afetando a motilidade.…”