Mating and hormonal triggers regulate accessory gland gene expression in male Drosophila

Herndon, Laura A.; Chapman, Tracey; Kalb, John M.; Lewin, Sharon R.; Partridge, Linda; Wolfner, Mariana F.

doi:10.1016/s0022-1910(97)00062-0

Cited by 72 publications

(63 citation statements)

References 39 publications

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“…The juvenile hormone-binding proteins from other insects, to which Takeout is related, are synthesized in the fat body and secreted into the hemolymph, where they are thought to carry juvenile hormone or other small lipophilic ligands to target cells (Nowock et al 1975;Glinka et al 1995;Wojtasek and Prestwich 1995). It is not known whether juvenile hormone plays any role in Drosophila male courtship behavior, but it has been shown that juvenile hormone stimulates the synthesis of accessory gland proteins (Herndon et al 1997). A role for juvenile hormone has also been found in the reproductive behavior of the Caribbean fruit fly Anastrepha suspense (Teal et al 2000).…”

Section: Genes and Development 2887mentioning

confidence: 99%

The Drosophila takeout gene is regulated by the somatic sex-determination pathway and affects male courtship behavior

Dauwalder¹,

Tsujimoto²,

Moss³

et al. 2002

Genes Dev.

190

254

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The Drosophila somatic sex-determination regulatory pathway has been well studied, but little is known about the target genes that it ultimately controls. In a differential screen for sex-specific transcripts expressed in fly heads, we identified a highly male-enriched transcript encoding Takeout, a protein related to a superfamily of factors that bind small lipophilic molecules. We show that sex-specific takeout transcripts derive from fat body tissue closely associated with the adult brain and are dependent on the sex determination genes doublesex (dsx) and fruitless (fru). The male-specific Doublesex and Fruitless proteins together activate Takeout expression, whereas the female-specific Doublesex protein represses takeout independently of Fru. When cells that normally express takeout are feminized by expression of the Transformer-F protein, male courtship behavior is dramatically reduced, suggesting that male identity in these cells is necessary for behavior. A loss-of-function mutation in the takeout gene reduces male courtship and synergizes with fruitless mutations, suggesting that takeout plays a redundant role with other fru-dependent factors involved in male mating behavior. Comparison of Takeout sequences to the Drosophila genome reveals a family of 20 related secreted factors. Expression analysis of a subset of these genes suggests that the takeout gene family encodes multiple factors with sex-specific functions.

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Section: Genes and Development 2887mentioning

confidence: 99%

The Drosophila takeout gene is regulated by the somatic sex-determination pathway and affects male courtship behavior

Dauwalder¹,

Tsujimoto²,

Moss³

et al. 2002

Genes Dev.

190

254

View full text Add to dashboard Cite

show abstract

“…However, assessing the production of these materials involves more than dissecting or homogenizing a male to determine whether he contains sperm and seminal-fluid proteins. One also needs to consider that wild-type males replenish their sperm and seminal fluid on a daily basis (DiBenedetto et al, 1990;Herndon et al, 1997), but not fast enough to avoid infertility after several matings in quick succession (Lefevre and Jonsson, 1962). This appears to be due to depletion of accessory-gland secretions but not of mature sperm (Hihara, 1981).…”

Section: Control Of Ejaculationmentioning

confidence: 99%

New reproductive anomalies in fruitless‐mutant Drosophila males: Extreme lengthening of mating durations and infertility correlated with defective serotonergic innervation of reproductive organs

et al. 2001

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Several features of male reproductive behavior are under the neural control of fruitless (fru) in Drosophila melanogaster. This gene is known to influence courtship steps prior to mating, due to the absence of attempted copulation in the behavioral repertoire of most types of fru-mutant males. However, certain combinations of fru mutations allow for fertility. By analyzing such matings and their consequences, we uncovered two striking defects: mating times up to four times the normal average duration of copulation; and frequent infertility, regardless of the time of mating by a given transheterozygous fru-mutant male. The lengthened copulation times may be connected with fru-induced defects in the formation of a male-specific abdominal muscle. Production of sperm and certain seminal fluid proteins are normal in these fru mutants. However, analysis of postmating qualities of females that copulated with transheterozygous mutants strongly implied defects in the ability of these males to transfer sperm and seminal fluids. Such abnormalities may be associated with certain serotonergic neurons in the abdominal ganglion in which production of 5HT is regulated by fru. These cells send processes to contractile muscles of the male's internal sex organs; such projection patterns are aberrant in the semifertile fru mutants. Therefore, the reproductive functions regulated by fruitless are expanded in their scope, encompassing not only the earliest stages of courtship behavior along with almost all subsequent steps in the behavioral sequence, but also more than one component of the culminating events.

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“…Corpora allata activity in drones, and consequently also the juvenile hormone titer, increase within the first days of adult life, and exhibit a peak during this period of sexual maturation (Tozetto et al, 1995;Giray and Robinson, 1996), one of the consequences being promotion of flight activity (Tozetto et al, 1997). Ecdysteroids, which play important roles in the reproductive physiology of males in many insect species, and have been studied extensively with regard to their effects on male accessory gland products Gillott, 1995, 1997;Herndon et al, 1997), have not been investigated in this context in honey bees. In the present study, the hemolymph ecdysteroid titer was determined for sexually immature and mature drones, and these titer analyses were then compared to the effects on protein concentration and pattern in mucus gland secretion observed after 20-hydroxyecdysone (20E) injection.…”

Section: Introductionmentioning

confidence: 99%

Protein content and pattern during mucus gland maturation and its ecdysteroid control in honey bee drones

Colonello-Frattini

Hartfelder

2003

Apidologie

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-We analyzed mucus gland protein content and pattern for drones of Africanized honey bees. The effect of exogenous ecdysteroids on mucus gland maturation was judged against the endogenous ecdysteroid titer. During the first 5 days of adult life, the mucus protein content increases steeply, whereas the protein pattern becomes reduced in complexity. Subsequently, the protein content decreases, reaching a plateau level at day 8. The protein pattern of mature glands is characterized by three dominant polypeptides. Injection of 20-hydroxyecdysone into newly emerged drones abolished the normal increase in protein content and prolonged the persistence of the protein pattern typical for immature glands. Ecdysteroids thus appear to act as negative regulators in the maturation process of drone mucus glands. This hypothesis received support from analyses of the hemolymph ecdysteroid titer, which was found to rapidly decline soon after emergence. male accessory gland / ecdysone / radioimmunoassay / Apis mellifera / mucus protein

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Mating and hormonal triggers regulate accessory gland gene expression in male Drosophila

Cited by 72 publications

References 39 publications

The Drosophila takeout gene is regulated by the somatic sex-determination pathway and affects male courtship behavior

The Drosophila takeout gene is regulated by the somatic sex-determination pathway and affects male courtship behavior

New reproductive anomalies in fruitless‐mutant Drosophila males: Extreme lengthening of mating durations and infertility correlated with defective serotonergic innervation of reproductive organs

Protein content and pattern during mucus gland maturation and its ecdysteroid control in honey bee drones

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