Matrine alters microRNA expression profiles in SGC-7901 human gastric cancer cells

LI, HAILONG; Xie, Shoupin; LIU, XIAOJUN; WU, HONGYAN; Lin, Xiaoyuan; Gu, Jiang; WANG, HUPING; Duan, Yun-you

doi:10.3892/or_xxxxxxxx

Cited by 29 publications

(13 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Acting as oncogenes or tumor suppressors, miRNAs can repress the expression of cancer-related genes (16). Several studies have reported that alterations in miRNA levels occur in various human tumors, including GC, where miRNAs are related to several aspects of cancer pathogenesis including self-renewal, invasion and metastasis by targeting EGR2 (17), MAPk (18) and PI3k/AkT signaling pathways (19). Therefore, we speculate that specific miRNAs may participate in the regulation of EGFR protein expression in GC.…”

Section: Introductionmentioning

confidence: 90%

miR-370 regulates cell proliferation and migration by targeting EGFR in gastric cancer

et al. 2017

View full text Add to dashboard Cite

Epidermal growth factor receptor (EGFR) is known to be overexpressed in ~30% of gastric cancer (GC) cases, and may serve as an effective biomarker for predicting the clinical benefit of anti-EGFR therapy. However, the mechanism underlying the regulation of EGFR expression remains unknown. Evidence indicates that post-transcriptional regulation may exist in the process of EGFR expression. In the present study, we aimed to explore whether miR-370 is involved in this process, and how it impacts the biological behaviors of GC cells. In the present study, we first determined the role of EGFR in GC by means of an EGFR overexpression plasmid and siRNAs. Then, the expression levels of EGFR protein and mRNA in GC tissues were analyzed through immunohistochemistry and quantitative RT-PCR (RT-qPCR). Bioinformatics tools and dual-luciferase assay were applied to predict and validate the relevant miRNA targeting EGFR. Finally, human GC MGC-803 cells were selected to explore the effect of miRNA on cell proliferation and migration. We found that suppression of EGFR inhibited the proliferation and migration of GC cells. In addition, the levels of EGFR protein in the GC tissues were ~4 times higher than that in the corresponding paired non-cancerous tissues while the mRNA levels of EGFR were only ~2-fold as high as that in the adjacent non-cancerous tissues. Bioinformatics tools predicted that miRNA-370 was a regulator of EGFR and dual‑luciferase assay validated that miR-370 could directly bind to the 3'-untranslated region (3'-UTR) of EGFR mRNA. Meanwhile, an inverse correlation between miR-370 and EGFR was found in the GC tissues. Overexpression of miR-370 suppressed the proliferation and migration of GC cells, while downregulation of miR-370 promoted proliferation and migration. The present study may provide new information for understanding the molecular mechanism underlying the regulation of EGFR protein expression in GC and may be of important clinical significance to guide targeted therapy.

show abstract

Section: Introductionmentioning

confidence: 90%

miR-370 regulates cell proliferation and migration by targeting EGFR in gastric cancer

et al. 2017

View full text Add to dashboard Cite

show abstract

“…MicroRNAs (miRNAs/miRs) are considered to have important roles in tumors. It has recently been reported that matrine alters miRNA expression profiles in SGC-7901 human gastric cancer cells, providing a novel and promising approach to identify the mechanisms of action of matrine (18). In this study, an miRNA microarray was used to screen relative miRNA levels of SGC-7901 human gastric cancer cells following matrine treatment (18).…”

Section: Introductionmentioning

confidence: 99%

Matrine exerts inhibitory effects in melanoma through the regulation of miR-19b-3p/PTEN

et al. 2018

View full text Add to dashboard Cite

Matrine, one of the main alkaloid components extracted from the traditional Chinese herb, Sophora flavescens Ait, has various pharmacological effects, and has been reported to exert antitumor activity in melanoma. In the current study, the molecular mechanisms underlying the inhibitory effects of matrine were investigated in melanoma cell line. It was initially confirmed that matrine inhibited proliferation, invasion and induced apoptosis in human A375 and SK-MEL-2 melanoma cell lines in vitro. Subsequently, reverse transcription-quantitative polymerase chain reaction (RT-qPCR) analysis demonstrated that the expression of microRNA (miR)-19b-3p was significantly increased in melanoma cells and was downregulated by treatment with matrine. Furthermore, downregulated miR-19b-3p exerted effects similar to 500 µg/ml matrine on cell proliferation, invasion and apoptosis. Phosphatase and tensin homolog (PTEN) mRNA was identified as a direct target of miR-19b-3p through bioinformatics analysis and a dual-luciferase reporter assay. Additionally, western blotting and RT-qPCR analysis demonstrated that the expression of PTEN protein and mRNA were increased by the treatment with matrine. Furthermore, silencing of PTEN expression reversed the effects of matrine and miR-19b-3p downregulation in A375 and SK-MEL-2 cells. Taken together, the results indicated that matrine may suppress cell proliferation and invasion and induce cell apoptosis partially via miR-19b-3p targeting of PTEN.

show abstract

“…Analyses showed that E2F-1 and Apaf-1 were upregulated and that caspase-9 and -3 were activated after the exposure to matrine [21] . Li et al [22] identified 128 miRNAs exhibiting >2-fold expression changes in matrine-treated cells relative to their expression levels in untreated cells. The data revealed that the majority of the 57 identified enrichment pathways were highly involved in tumorigenesis.…”

Section: Introductionmentioning

confidence: 99%

Anti-tumor activities of active ingredients in Compound Kushen Injection

Wang¹,

et al. 2015

View full text Add to dashboard Cite

Kushen (Radix Sophorae Flavescentis) has a long history of use for the treatment of tumors, inflammation and other diseases in traditional Chinese medicine. Compound Kushen Injection (CKI) is a mixture of natural compounds extracted from Kushen and Baituling (Rhizoma Smilacis Glabrae). The main principles of CKI are matrine (MT) and oxymatrine (OMT) that exhibit a variety of pharmacological activities, including anti-inflammatory, anti-allergic, anti-viral, anti-fibrotic and cardiovascular protective effects. Recent evidence shows that these compounds also produce anti-cancer actions, such as inhibiting cancer cell proliferation, inducing cell cycle arrest, accelerating apoptosis, restraining angiogenesis, inducing cell differentiation, inhibiting cancer metastasis and invasion, reversing multidrug resistance, and preventing or reducing chemotherapy-and/or radiotherapy-induced toxicity when combined with chemotherapeutic drugs. In this review, we summarize recent progress in studying the anti-cancer activities of MT, OMT and CKI and their potential molecular targets, which provide clues and references for further study.

show abstract

Matrine alters microRNA expression profiles in SGC-7901 human gastric cancer cells

Cited by 29 publications

References 38 publications

miR-370 regulates cell proliferation and migration by targeting EGFR in gastric cancer

miR-370 regulates cell proliferation and migration by targeting EGFR in gastric cancer

Matrine exerts inhibitory effects in melanoma through the regulation of miR-19b-3p/PTEN

Anti-tumor activities of active ingredients in Compound Kushen Injection

Contact Info

Product

Resources

About