Matrine Reduces Proliferation of Human Lung Cancer Cells by Inducing Apoptosis and Changing miRNA Expression Profiles

Liu, Yongqi; Li, Yang; Qin, Jie; Wang, Qian; She, Yali; Luo, Yali; He, Jianxin; Li, Jing-Ya; Xie, Xiaodong

doi:10.7314/apjcp.2014.15.5.2169

Cited by 27 publications

(17 citation statements)

References 55 publications

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“…According to nasopharyngeal darcinoma, miR-29c could promote the apoptosis of cancer cells and increase the sensitivity of cancer cells to radiotherapy and chemotherapy . In human lung cancer cells, matrine could reduce proliferation of cancer cells by inducing apoptosis and down-regulating the expression of BCL-2, author detected the expression of miR and found that the mechanism may be associated with the up-regulating of miR-29 c (Liu et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

Exosome-derived microRNA-29c Induces Apoptosis of BIU-87 Cells by Down Regulating BCL-2 and MCL-1

Xu¹,

Wu²,

Fan³

et al. 2014

Asian Pacific Journal of Cancer Prevention

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Asian Pac J Cancer Prev, 15 (8), 3471-3476 IntroductionThe incidence of bladder carcinoma is in the first in Chinese urological tumor, which includes the 70% non invasive cancer and 30% invasive cancer. The patients accompanied with systemic metastasis have poor prognosis and a lifetime risk of recurrence.Tumor-derived exosomes had been regarded as a good carrier of anti-tumor therapy for its advantages of tumor specific antigen recognition, easy to obtain. For example, exosomes from tumor cells treated by heat shock or genetic modification were associated with ODN-CpG adjuvants to deal with the tumor bearing mice, showing that it enhanced the antitumor activity of the secretion of exosomes (Chaput et al., 2004). Researches on exosomesderived miRNA indicated that it could be used as a new

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Section: Discussionmentioning

confidence: 99%

Exosome-derived microRNA-29c Induces Apoptosis of BIU-87 Cells by Down Regulating BCL-2 and MCL-1

Xu¹,

Wu²,

Fan³

et al. 2014

Asian Pacific Journal of Cancer Prevention

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“…The upregulation of Bax and downregulation of Bcl-2 can cause apoptosis of human colon cancer cells in mitochondrial apoptotic pathway (Chang et al, 2013). Our previous Western blotting results showed matrine can inhibit the protein expression of bcl-2, VEGF, HDAC1 and increase the protein expression of bax, down regulate the ratio of bcl-2/bax, promoting apotosis of A549 cancer cells, inhibiting the proliferation of A549 cancer cells (Liu et al, 2014). Kuraridin and nor-kurarinone isolated from Sophora flavescens can also induce apoptosis of human gastric adenocarcinoma SGC-7901 cells (Rasul et al, 2011).…”

Section: Discussionmentioning

confidence: 94%

Tumor Inhibition Effects and Mechanisms of Angelica sinensis and Sophorae flavescentis ait Decoction Combined with Cisplatin in Xenograft Mice

Yan

Liu²,

Li³

et al. 2014

Asian Pacific Journal of Cancer Prevention

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“…Bcl-2 and Bax are two important proteins and play role in mitochondrial-mediated pathway (Huang et al, 2014). The ratio between Bcl-2 and Bax expression level determines whether cells respond to an apoptotic signal (Liu et al, 2014). In contrast to Bcl-2, Bax protein accumulation in high amounts in the cell initiates apoptosis.…”

Section: Discussionmentioning

confidence: 99%

Effects of Hypobaric Conditions on Apoptosis Signalling Pathways in HeLa Cells

Arıcan

Khalilia²,

Serbes³

et al. 2014

Asian Pacific Journal of Cancer Prevention

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Nowadays increasing effectiveness in cancer therapy and investigation of formation of new strategies that enhance antiproliferative activity against target organs has become a subject of interest. Although the molecular mechanisms of apoptosis can not be fully explained, it is known that cell suicide program existing in their memory genetically is activated by pathophysiological conditions and events such as oxidative stress. Low pressure (hypobaric) conditions that create hypoxia promote apoptosis by inhibiting cell cycling. In this study, determination of the effects of fractional hypobaric applications at different times on HeLa cells at cellular and molecular levels were targeted. Experiments were carried out under hypobaric conditions (35.2 kPa) in a specially designed hypobaric cabin including 2% O 2 and 98% N. Application of fractional hypobaric conditions was repeated two times for 3 hours with an interval of 24 hours. At the end of the implementation period cells were allowed to incubate for 24 hours for activation of repair mechanisms. Cell kinetic parameters such as growth rate (MTT) and apoptotic index were used in determination of the effect of hypobaric conditions on HeLa cells. Also in our study expression levels of the Bcl-2 gene family that have regulatory roles in apoptosis were determined by the RT-PCR technique to evaluate molecular mechanisms. The results showed that antiproliferative effect of hypobaric conditions on HeLa cells started three hours from the time of application and increased depending on the period of exposure. While there was a significant decrease in growth rate values, there was a significant increase in apoptotic index values (p<0.01). Also molecular studies showed that hypobaric conditions caused a significant increase in expression level of proapoptotic gene Bax and significant decrease in antiapoptotic Bfl-1. Consequently fractional application of hypobaric conditions on HeLa cell cultures increased both antiproliferative and apoptotic effects and these effects were triggered by the Bax gene.

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Matrine Reduces Proliferation of Human Lung Cancer Cells by Inducing Apoptosis and Changing miRNA Expression Profiles

Cited by 27 publications

References 55 publications

Exosome-derived microRNA-29c Induces Apoptosis of BIU-87 Cells by Down Regulating BCL-2 and MCL-1

Exosome-derived microRNA-29c Induces Apoptosis of BIU-87 Cells by Down Regulating BCL-2 and MCL-1

Tumor Inhibition Effects and Mechanisms of Angelica sinensis and Sophorae flavescentis ait Decoction Combined with Cisplatin in Xenograft Mice

Effects of Hypobaric Conditions on Apoptosis Signalling Pathways in HeLa Cells

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