Matrix-assisted laser desorption/ionisation time-of-flight (MALDI-TOF) mass spectrometry as a tool for rapid diagnosis of potentially toxigenic Corynebacterium species in the laboratory management of diphtheria-associated bacteria

Konrad, Regina; Berger, Anja; Huber, Ingrid; Boschert, V.; Hörmansdorfer, Stefan; Busch, Ulrich; Hogardt, Michael; Schubert, Sören; Sing, Andreas

doi:10.2807/ese.15.43.19699-en

Cited by 107 publications

(91 citation statements)

References 17 publications

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“…The spectrum generated by the MS process is compared with reference spectra and a specific identification can be made if the isolate is a species included in the reference database. In a study of 116 Corynebacterium species isolates submitted to the German Consiliary Laboratory, MALDI-TOF MS showed agreement with rpoB gene sequencing for 115 of 116 isolates (99.1 %) (Konrad et al, 2010). The only isolate for which MALDI-TOF MS was accurate only to the genus level was one identified by rpoB as Corynebacterium tuberculostearicum.…”

Section: Discussionmentioning

confidence: 94%

Characterization of Corynebacterium species in macaques

Venezia

Cassiday

Marini

et al. 2012

Journal of Medical Microbiology

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Bacteria of the genus Corynebacterium are important primary and opportunistic pathogens. Many are zoonotic agents. In this report, phenotypic (API Coryne analysis), genetic (rpoB and 16S rRNA gene sequencing), and physical methods (MS) were used to distinguish the closely related diphtheroid species Corynebacterium ulcerans and Corynebacterium pseudotuberculosis, and to definitively diagnose Corynebacterium renale from cephalic implants of rhesus (Macaca mulatta) and cynomolgus (Macaca fascicularis) macaques used in cognitive neuroscience research. Throat and cephalic implant cultures yielded 85 isolates from 43 macaques. Identification by API Coryne yielded C. ulcerans (n574), Corynebacterium pseudotuberculosis (n52), C. renale or most closely related to C. renale (n53), and commensals and opportunists (n56). The two isolates identified as C. pseudotuberculosis by API Coryne required genetic and MS analysis for accurate characterization as C. ulcerans. Of three isolates identified as C. renale by 16S rRNA gene sequencing, only one could be confirmed as such by API Coryne, rpoB gene sequencing and MS. This study emphasizes the importance of adjunct methods in identification of coryneforms and is the first isolation of C. renale from cephalic implants in macaques.

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Section: Discussionmentioning

confidence: 94%

Characterization of Corynebacterium species in macaques

Venezia

Cassiday

Marini

et al. 2012

Journal of Medical Microbiology

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“…This technology is touted as being revolutionary, because it does not require extensive training or expertise in MS or chemistry to use and the cost for consumables is described as less than US$0.50 per sample, excluding the cost of the MALDI-TOF instrument (21). Protein fingerprints are deemed to provide identification to genus and species with an accuracy which approaches that of genetic methods (1,29). There is limited data evaluating the use of this tool for the identification of Corynebacterium spp.…”

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confidence: 99%

“…Matrixassisted laser desorption ionization-time of flight (MALDI-TOF) analysis is rapidly becoming a routinely used tool in many microbiology laboratories, whereby proteins liberated from bacteria are ionized and detected by a mass spectrometer (MS), the spectrum is analyzed, and its pattern is compared to entries found in a database, giving rise to a degree of match (1,29). This technology is touted as being revolutionary, because it does not require extensive training or expertise in MS or chemistry to use and the cost for consumables is described as less than US$0.50 per sample, excluding the cost of the MALDI-TOF instrument (21).…”

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confidence: 99%

“…(i) The bacteria are misidentified or not identified compared to identification using genetically based methods (1,29), with Ͼ30% of isolates being incorrectly identified without ancillary testing or not identified at all (2). (ii) There are a limited number of substrates in the panels, which does not provide a sufficient number of unique features to differentiate among all relevant taxa.…”

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confidence: 99%

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The Genus Corynebacterium and Other Medically Relevant Coryneform-Like Bacteria

2012

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Catalase-positive Gram-positive bacilli, commonly called “diphtheroids” or “coryneform” bacteria were historically nearly always dismissed as contaminants when recovered from patients, but increasingly have been implicated as the cause of significant infections. These taxa have been underreported, and the taxa were taxonomically confusing. The mechanisms of pathogenesis, especially for newly described taxa, were rarely studied. Antibiotic susceptibility data were relatively scant. In this minireview, clinical relevance, phenotypic and genetic identification methods, matrix-assisted laser desorption ionization–time of flight (MALDI-TOF) evaluations, and antimicrobial susceptibility testing involving species in the genus Corynebacterium and other medically relevant Gram-positive rods, collectively called coryneforms, are described.

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“…It also can be used to screen biomarkers in complex systems including serum [16,22]. It plays a key role in the toxin's diagnosis and therapy [23,24]. Nanoscale Ultra Performance liquid chromatography coupled to tandem mass spectrometry (NanoUPLC-MS/MS) was used for determination of ricin in solution because of its high sensitivity and the acquisition of reliable data from independent protein digests.…”

Section: Introductionmentioning

confidence: 99%

Rapid Detection of Ricin in Serum Based on Cu-Chelated Magnetic Beads Using Mass Spectrometry

Zhao

Song

Wang

et al. 2016

J. Am. Soc. Mass Spectrom.

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Abstract. The protein toxin ricin obtained from castor bean plant (Ricinus communis) seeds is a potent biological warfare agent due to its ease of availability and acute toxicity.In this study, we demonstrated a rapid and simple method to detect ricin in serum in vitro. The ricin was mixed with serum and digested by trypsin, then all the peptides were efficiently extracted using Cu-chelated magnetic beads and were detected with matrixassisted laser desorption/ionization time-of-flight mass spectrometry. The specific ricin peptides were identified by Nanoscale Ultra Performance liquid chromatography coupled to tandem mass spectrometry according to their sequences. The assay required 2.5 hours, and a characteristic peptide could be detected down to 4 ng/μl and used as a biomarker to detect ricin in serum. The high sensitivity and simplicity of the procedure makes it valuable in clinical practice.

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Matrix-assisted laser desorption/ionisation time-of-flight (MALDI-TOF) mass spectrometry as a tool for rapid diagnosis of potentially toxigenic Corynebacterium species in the laboratory management of diphtheria-associated bacteria

Abstract: S, Sing A. Matrix-assisted laser desorption/ionisation time-of-flight (MALDI-TOF) mass spectrometry as a tool for rapid diagnosis of potentially toxigenic Corynebacterium species in the laboratory management of diphtheriaassociated bacteria.

Cited by 107 publications

References 17 publications

Characterization of Corynebacterium species in macaques

Characterization of Corynebacterium species in macaques

The Genus Corynebacterium and Other Medically Relevant Coryneform-Like Bacteria

Rapid Detection of Ricin in Serum Based on Cu-Chelated Magnetic Beads Using Mass Spectrometry

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