Matrix-assisted laser desorption ionization-time of flight mass spectrometry analysis of Escherichia coli categories

Dallagassa, Cibelle B.; Huergo, Luciano F.; Stets, Maria Isabel; Pedrosa, Fábio O.; Souza, Emanuel Maltempi de; Cruz, Leonardo M.; Assis, Flávia E.A.; Wolf, Susan M.; Volanski, W.; Picheth, Geraldo; Pigatto-Denardi, C.P.; Farah, S.M.S.S.; Picheth, Geraldo

doi:10.4238/2014.january.29.2

Cited by 10 publications

(5 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For the analyzed strains, the range of mass peaks obtained was of approximately between 2,000 and 11,000 m/z, of which intraspecific similarities and distinctions are observed in Table 2 and Figure 2. The results obtained for the strains analyzed were similar to those already described for Escherichia coli strains [10,14,15,17,18,19,20,26,32].…”

Section: Resultssupporting

confidence: 85%

“…This method has been rapidly expanded and successfully applied in the field of identification of microbial clinical specimens in European countries and North America, in a fast, stable and cost-effective way [15,24]. The use of MALDI TOF for the identification of different strains of Escherichia coli is described in the literature [10,15,17,25,26,32]. Thus, the purpose of the present study was to verify the ability of ERIC-PCR and MALDI-TOF methods to discriminate between different lineages of Escherichia coli strains including lineages of Escherichia coli K-12.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Genomic and proteomic approach as a tool to discrimination of Escherichia coli strains of biotechnological interest

Barbosa¹,

Leite²,

Xavier³

et al. 2019

jabb

View full text Add to dashboard Cite

The Methods based on genomic and proteomic approaches are described as effective tools for the identification of microorganisms. The development of methodologies capable of differentiating, interspecifically, pathogenic, wild and genetically modified Escherichia coli strains is desirable for the fields of healthcare and Biotechnology. The purpose of this study was to verify the viability of ERIC-PCR and MALDI TOF methods in differentiating lineages of Escherichia strains. For this purpose, laboratory Escherichia coli ATCC8739, Escherichia coli W3110, Escherichia coli BL21DE3+, Escherichia coli JM109, Escherichia coli MC 1061 and Escherichia coli DH5α were subjected to ERIC-PCR and MALDI TOF mass spectrometry analyzes. Genomic (ERIC-PCR) and proteomic (MALDI-TOF) methods were able to discriminate between different lineages of Escherichia coli strains including lineages of Escherichia coli K-12. However, the MALDI TOF proteomic approach revealed being able to differentiate interspecifically lineages of Escherichia coli strains. The determination of the most frequent masses found in the studied Escherichia coli strains in addition to future experiments of peptide sequencing profile and SDS-PAGE can be used as a guideline for validating a method for proteomic identification of these strains.

show abstract

Section: Resultssupporting

confidence: 85%

Section: Introductionmentioning

confidence: 99%

Genomic and proteomic approach as a tool to discrimination of Escherichia coli strains of biotechnological interest

Barbosa¹,

Leite²,

Xavier³

et al. 2019

jabb

View full text Add to dashboard Cite

show abstract

“…Raw data were converted into peak lists using FlexAnalysis 3.0 (Bruker-Daltonics, Bremen, Germany). Cell extracts (38,39) were spotted on three different positions of the MALDI plate, generating at least three spectra per strain. Matrix was 10 mg/ml ␣-cyano-4-hydroxycinnamic acid in 50% acetonitrile-2.5% trifluoroacetic acid.…”

mentioning

confidence: 99%

Biochemical Characteristics, Adhesion, and Cytotoxicity of Environmental and Clinical Isolates of Herbaspirillum spp

et al. 2015

View full text Add to dashboard Cite

e Herbaspirillum bacteria are best known as plant growth-promoting rhizobacteria but have also been recovered from clinical samples. Here, biochemical tests, matrix-assisted laser deionization-time of flight (MALDI-TOF) mass spectrometry, adherence, and cytotoxicity to eukaryotic cells were used to compare clinical and environmental isolates of Herbaspirillum spp. Discrete biochemical differences were observed between human and environmental strains. All strains adhered to HeLa cells at low densities, and cytotoxic effects were discrete, supporting the view that Herbaspirillum bacteria are opportunists with low virulence potential.

show abstract

“…In detection of Salmonella Enteritidis, proteomic approaches have been used to detect isogenic Type Three Secretion System-I (TTSS-I) known as M1511 and its wild-type isolate, P125109 using a high-resolution Fourier transform mass spectrometer [ 156 ]. For Escherichia coli identification, matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-ToF MS) can also be used because it is a rapid, low cost, reliable and accurate proteomic method for E. coli identification up to the subspecies level compared with classical culture microbiological techniques [ 157 , 158 ].…”

Section: Omics-based Technologies For Age Diagnosis and Managementmentioning

confidence: 99%

Acute Infectious Gastroenteritis: The Causative Agents, Omics-Based Detection of Antigens and Novel Biomarkers

et al. 2021

View full text Add to dashboard Cite

Acute infectious gastroenteritis (AGE) is among the leading causes of mortality in children less than 5 years of age worldwide. There are many causative agents that lead to this infection, with rotavirus being the commonest pathogen in the past decade. However, this trend is now being progressively replaced by another agent, which is the norovirus. Apart from the viruses, bacteria such as Salmonella and Escherichia coli and parasites such as Entamoeba histolytica also contribute to AGE. These agents can be recognised by their respective biological markers, which are mainly the specific antigens or genes to determine the causative pathogen. In conjunction to that, omics technologies are currently providing crucial insights into the diagnosis of acute infectious gastroenteritis at the molecular level. Recent advancement in omics technologies could be an important tool to further elucidate the potential causative agents for AGE. This review will explore the current available biomarkers and antigens available for the diagnosis and management of the different causative agents of AGE. Despite the high-priced multi-omics approaches, the idea for utilization of these technologies is to allow more robust discovery of novel antigens and biomarkers related to management AGE, which eventually can be developed using easier and cheaper detection methods for future clinical setting. Thus, prediction of prognosis, virulence and drug susceptibility for active infections can be obtained. Case management, risk prediction for hospital-acquired infections, outbreak detection, and antimicrobial accountability are aimed for further improvement by integrating these capabilities into a new clinical workflow.

show abstract

Matrix-assisted laser desorption ionization-time of flight mass spectrometry analysis of Escherichia coli categories

Cited by 10 publications

References 19 publications

Genomic and proteomic approach as a tool to discrimination of Escherichia coli strains of biotechnological interest

Genomic and proteomic approach as a tool to discrimination of Escherichia coli strains of biotechnological interest

Biochemical Characteristics, Adhesion, and Cytotoxicity of Environmental and Clinical Isolates of Herbaspirillum spp

Acute Infectious Gastroenteritis: The Causative Agents, Omics-Based Detection of Antigens and Novel Biomarkers

Contact Info

Product

Resources

About