Matrix effect screening for cloud-point extraction combined with liquid chromatography coupled to mass spectrometry: Bioanalysis of pharmaceuticals

Kojro, Grzegorz; Rudzki, Piotr J.; Pisklak, Dariusz Maciej; Giebułtowicz, Joanna

doi:10.1016/j.chroma.2019.01.031

Cited by 14 publications

(11 citation statements)

References 41 publications

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“…In contrast to 6%, at 1.5% Triton X-114 concentration, ME A was not observed for DES, MAP, MIA, MOC, and OPI. Higher surfactant concentration is related to a higher risk of ME A , as reported previously [31]. The possible reason is the interference of surfactant with droplet evaporation or higher recovery not only of analytes, but also impurities of samples that interfere with the analyte ionization.…”

Section: The Effects Of Triton X-114supporting

confidence: 57%

“…Some compounds revealed significant ME A in all tested conditions (CIT, DOX, TRA, VEN). All of them showed a significant surfactant effect (the ratio of peak area of the analyte in a solvent with the surfactant to that without the surfactant) previously [31]. That means that the ME A observed is not related to impurities extracted from plasma, but the presence of Triton in the sample.…”

Section: Principal Component Analysis (Pca)mentioning

confidence: 84%

“…In this paper, the influences of surfactant concentration (Triton X-114), volatile salt (AA-0, 10, 20, and 30% (w/v)), and extraction pH (about 3.5, 6.8, 10.2) was tested to select the optimal conditions for the simultaneous determination of 21 antidepressants. Other parameters, i.e., volume of reagents and equilibrium time, were used as described previously [2,31]. However, in the current study higher temperature was selected (60 • C).…”

Section: Development Of Cloud-point Extractionmentioning

confidence: 99%

“…The chromatography separation was not optimized. We used the gradient elution mode, eluent flow rate, and analytical column commonly used in our analysis [31].…”

Section: Development Of Cloud-point Extractionmentioning

confidence: 99%

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Determination of Antidepressants in Human Plasma by Modified Cloud-Point Extraction Coupled with Mass Spectrometry

et al. 2020

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Cloud-point extraction (CPE) is rarely combined with liquid chromatography coupled to mass spectrometry (LC–MS) in drug determination due to the matrix effect (ME). However, we have recently shown that ME is not a limiting factor in CPE. Low extraction efficiency may be improved by salt addition, but none of the salts used in CPE are suitable for LC–MS. It is the first time that the influences of a volatile salt—ammonium acetate (AA)—on the CPE extraction efficiency and ME have been studied. Our modification of CPE included also the use of ethanol instead of acetonitrile to reduce the sample viscosity and make the method more environmentally friendly. We developed and validated CPE–LC–MS for the simultaneous determination of 21 antidepressants in plasma that can be useful for clinical and forensic toxicology. The selected parameters included Triton X-114 concentration (1.5 and 6%, w/v), concentration of AA (0, 10, 20 and 30%, w/v), and pH (3.5, 6.8 and 10.2). The addition of 10% of AA increased recovery twice. For 20 and 30% (w/v) of AA, three phases were formed that prolonged the extraction process. The developed CPE method (6% Triton X-114, 10% AA, pH 10.2) was successfully validated through LC–MS/MS simultaneous determination of 21 antidepressants in human plasma. The linearity was in the range of 10–750 ng/mL (r2 > 0.990).

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Section: The Effects Of Triton X-114supporting

confidence: 57%

Section: Principal Component Analysis (Pca)mentioning

confidence: 84%

Section: Development Of Cloud-point Extractionmentioning

confidence: 99%

“…The chromatography separation was not optimized. We used the gradient elution mode, eluent flow rate, and analytical column commonly used in our analysis [31].…”

Section: Development Of Cloud-point Extractionmentioning

confidence: 99%

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Determination of Antidepressants in Human Plasma by Modified Cloud-Point Extraction Coupled with Mass Spectrometry

et al. 2020

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“…Despite that, some parameters in this procedure need to be optimized, including surfactant concentration, pH, extraction time, and temperature. Furthermore, it is believed that the CPE method can be extensively applied because of its simplicity, rapid execution speed, and least environmental impact (Giebultowicz et al, 2015;Giebultowicz, Kojro, Piotrowski, Kulakowski, & Wroczynski, 2016;Kojro, Rudzki, Pisklak, & Giebultowicz, 2019).…”

Section: Conventional Pretreatment Methodsmentioning

confidence: 99%

Technologies to improve the sensitivity of existing chromatographic methods used for bioanalytical studies

Chen

Gao

Zhong

2020

Biomedical Chromatography

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Chromatographic method has long been recognized as the most widely used separation method in bioanalytical research. However, the relatively low sensitivity of existing chromatographic methods remains a significant challenge, as the requirements for experimental procedures become more demanding. This review discusses the main causes for the low sensitivity of chromatographic methods and aims to introduce different technologies for enhancing their sensitivity in the following aspects: (i) different pretreatment methods for improving clean‐up efficiency and recovery; (ii) derivatization step for altering the chromatographic behavior of analytes and enhancing MS ionization efficiency; (iii) optimal LC–MS conditions and appropriate separation mechanism; and (iv) applications of other chromatographic methods, including miniaturized LC, 2D‐LC, 2D‐GC, and supercritical fluid chromatography. Altogether, this review is devoted to summarizing the recent technologies reported in the literature and providing new strategies for the detection of bioanalytes.

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Twenty years of supramolecular solvents in sample preparation for chromatography: achievements and challenges ahead

Rubio

2020

Anal Bioanal Chem

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Matrix effect screening for cloud-point extraction combined with liquid chromatography coupled to mass spectrometry: Bioanalysis of pharmaceuticals

Cited by 14 publications

References 41 publications

Determination of Antidepressants in Human Plasma by Modified Cloud-Point Extraction Coupled with Mass Spectrometry

Determination of Antidepressants in Human Plasma by Modified Cloud-Point Extraction Coupled with Mass Spectrometry

Technologies to improve the sensitivity of existing chromatographic methods used for bioanalytical studies

Twenty years of supramolecular solvents in sample preparation for chromatography: achievements and challenges ahead

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