Matrix metalloproteinases (MMP) - MMP-1,-2,-9 and its endogenous activity regulators in transformed by E7 oncogene HPV16 and HPV18 cervical carcinoma cell lines

Ryzhakova, O. S.; Solov'eva, N. I.

doi:10.18097/pbmc20135905530

Cited by 14 publications

(11 citation statements)

References 15 publications

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“…HeLa cell lines are transformed by Human papillomavirus 18 [ 24 ], and Human papillomavirus types 16 and 18 are the most common strains in Taiwan, accounting for nearly 70% of all cervical cancer cases [ 1 ]. Acute myeloid leukemia (AML) is the most common form of leukemia in adults; therefore, human AML cell line U937 was used as the raw material in our study.…”

Section: Resultsmentioning

confidence: 99%

Linalool Induces Cell Cycle Arrest and Apoptosis in Leukemia Cells and Cervical Cancer Cells through CDKIs

Chang

Shieh

Chen

et al. 2015

IJMS

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Plantaginaceae, a popular traditional Chinese medicine, has long been used for treating various diseases from common cold to cancer. Linalool is one of the biologically active compounds that can be isolated from Plantaginaceae. Most of the commonly used cytotoxic anticancer drugs have been shown to induce apoptosis in susceptible tumor cells. However, the signaling pathway for apoptosis remains undefined. In this study, the cytotoxic effect of linalool on human cancer cell lines was investigated. Water-soluble tetrazolium salts (WST-1) based colorimetric cellular cytotoxicity assay, was used to test the cytotoxic ability of linalool against U937 and HeLa cells, and flow cytometry (FCM) and genechip analysis were used to investigate the possible mechanism of apoptosis. These results demonstrated that linalool exhibited a good cytotoxic effect on U937 and HeLa cells, with the IC50 value of 2.59 and 11.02 μM, respectively, compared with 5-FU with values of 4.86 and 12.31 μM, respectively. After treating U937 cells with linalool for 6 h, we found an increased sub-G1 peak and a dose-dependent phenomenon, whereby these cells were arrested at the G0/G1 phase. Furthermore, by using genechip analysis, we observed that linalool can promote p53, p21, p27, p16, and p18 gene expression. Therefore, this study verified that linalool can arrest the cell cycle of U937 cells at the G0/G1 phase and can arrest the cell cycle of HeLa cells at the G2/M phase. Its mechanism facilitates the expression of the cyclin-dependent kinases inhibitors (CDKIs) p53, p21, p27, p16, and p18, as well as the non-expression of cyclin-dependent kinases (CDKs) activity.

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Section: Resultsmentioning

confidence: 99%

Linalool Induces Cell Cycle Arrest and Apoptosis in Leukemia Cells and Cervical Cancer Cells through CDKIs

Chang

Shieh

Chen

et al. 2015

IJMS

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“…The dissolution of the basement membrane caused by MMP in tumors is a notable process in the movement of tumor cells. A significant correlation has been reported between the activation of MMP and the migration and invasion of tumor cells ( 32 , 33 ). The expression of MMP2 and MMP9 was observed to be higher in patients with lymph node metastases compared with patients without lymph node involvement in oropharyngeal SCC ( 34 ).…”

Section: Discussionmentioning

confidence: 99%

miR-199a-5p induces cell invasion by suppressing E-cadherin expression in cutaneous squamous cell carcinoma

Wang

Cao

et al. 2016

Oncology Letters

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A growing quantity of evidence exists to suggest that microRNAs are significant regulators of multiple cellular processes. When expressed aberrantly in different types of cancer, including cutaneous squamous cell carcinoma (cSCC), they play key roles in tumorigenesis and progression. The aberrant expression of miR-199a-5p has been observed to contribute to carcinogenesis in various types of cancer. However, the role of miR-199a-5p in the progression of cSCC metastasis remains largely unknown. In this study, we determined that miR-199a-5p was the upstream regulator of CDH1 (E-cadherin) and that it could suppress the expression of E-cadherin in cSCC cells. In addition, miR-199a-5p mimics significantly induced cell invasion and the activity of matrix metalloproteinase (MMP)2 and MMP9 in cSCC cells. In conclusion, these results are likely to aid in elucidating the molecular mechanisms of cSCC progression. In addition, the findings provide a new theoretical basis to further investigate miR-199a-5p as a potential biomarker and a promising approach in cSCC treatment.

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“…Previously, we found that the transformation of broblasts with the E7 HPV16 oncogene was accompanied by the induction of MMP-1 expression [42,45]. The study of MMP expression, carried out by us on E7 HPV16-associated samples of PCSM tumors, showed that the main contribution to the invasive and metastatic potential of the tumor is made by an increase in the expression of collagenases and, rst of all, MMP-1 along with MMP-9, which can serve as markers of invasive and metastatic potential tumors [44][45][46]. In previous our studies, the expression of MMP-1 and EMN in SCC was found both in the cervical tumor and in the stroma of the tumor and uterine tissue, and the expression of MMP-1 in normal tissue was at a lower level than in the tumor, and the level of EMN expression did not change signi cantly.…”

Section: Discussionmentioning

confidence: 99%

Interstitial collagenase MMP-1 and EMMPRIN in cell lines transformed with HPV16 and HPV18, and clinical specimens of cervical squamous cell carcinoma

Timoshenko

Solovyeva

Kugaevskaya

et al. 2021

Preprint

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Background: The aim of this study was to elucidate the features of expression of the inducer of expression of matrix metalloproteinases – EMMPRIN (EMN) and matrix metalloproteinase - MMP-1 in cell lines transformed with HPV16 and HPV18, and clinical samples of squamous cell carcinoma of the cervix uteri (SCC), as factors of tumor invasion.Material and methods: The study was carried out using RT-PCR and immunohistochemical studies (IHC).Results: The data obtained indicate that the expression of mRNA EMN and MMP-1 occurs in all cell lines. No direct correlation was found between the expression of EMN and MMP-1. Expression of mRNA of both EMN and MMP-1 in the HPV18 cell lines was more pronounced than in HPV16. The expression of MMP-1 in tumor tissue of SCC, as a rule, was significantly higher than in normal tissue, according to densitometry data by 5-7 times. The high MMP-1 mRNA expression was found in 90% of tumor samples. Expression of EMN mRNA was found in tumor and normal tissues. In most tumor samples, expression was at or below normal tissue level, and an increase in expression was detected in only isolated cases of SCC. Conclusion: The obtained data are important for understanding the process of tumor development and may have prognostic value. MMP-1 can serve as a marker of the invasive potential of SCC.

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Matrix metalloproteinases (MMP) - MMP-1,-2,-9 and its endogenous activity regulators in transformed by E7 oncogene HPV16 and HPV18 cervical carcinoma cell lines

Cited by 14 publications

References 15 publications

Linalool Induces Cell Cycle Arrest and Apoptosis in Leukemia Cells and Cervical Cancer Cells through CDKIs

Linalool Induces Cell Cycle Arrest and Apoptosis in Leukemia Cells and Cervical Cancer Cells through CDKIs

miR-199a-5p induces cell invasion by suppressing E-cadherin expression in cutaneous squamous cell carcinoma

Interstitial collagenase MMP-1 and EMMPRIN in cell lines transformed with HPV16 and HPV18, and clinical specimens of cervical squamous cell carcinoma

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