Maturation of Fetal Human Neural Xenografts in the Adult Rat Brain

Belkadi, Abdelaziz; Gény, Christian; Naimi, Souad; Jeny, R.; Peschanski, Marc; Riché, D.

doi:10.1006/exnr.1997.6414

Cited by 22 publications

(6 citation statements)

References 40 publications

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“…2,7,16,18,25,70 Although many studies have shown that immunosuppression prolonged survival of xenografts, 6,12,56,72 there is evidence that common immunosuppression agents like FK506 and cyclosporin A may play a neuroprotective role in other acute neurodegenerative conditions, including cerebral ischemia. 8,11,32,73 Therefore, to limit outcome variables, immunosuppression was not included in this experimental paradigm, and the posttransplantation survival interval was selected partially based on previous data on immunocompetent animals.…”

Section: Discussionmentioning

confidence: 98%

Survival and integration of transplanted postmitotic human neurons following experimental brain injury in immunocompetent rats

Philips¹,

Muir²,

Saatman³

et al. 1999

Journal of Neurosurgery

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Section: Discussionmentioning

confidence: 98%

Survival and integration of transplanted postmitotic human neurons following experimental brain injury in immunocompetent rats

Philips¹,

Muir²,

Saatman³

et al. 1999

Journal of Neurosurgery

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“…In contrast, glial cells possessed dark or higher electron dense nuclei without clearly visible nucleoli. Chromatin was highly concentrated along the nuclear membrane, which made the nuclear membrane stand out of the background as a dark ring [26,27]. In this culture system, astrocytes and oligodendrocytes could not be distinguished based on their ultrastructural features.…”

Section: Synaptogenesis Of Nscsmentioning

confidence: 93%

Synaptic transmission of neural stem cells seeded in 3-dimensional PLGA scaffolds

Yu¹,

Zeng²,

Zeng³

et al. 2009

Biomaterials

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“…Sources for cell transplantation in the nervous system includes fetal neural tissues [1], [2], [3], embryonic stem (ES) cells [4], [5], induced pluripotent stem (IPS) cells [6], neural stem cells (NSCs) [7], [8], non-neural somatic stem cells [9], [10], [11], [12] or even direct conversion of non-neural cells into neurons [13]. Each of these cell types have the potential to replace cells lost to injury or disease [14] or to modulate brain or spinal cord function [15]; with each having their own advantages and disadvantages.…”

Section: Introductionmentioning

confidence: 99%

Purification of Immature Neuronal Cells from Neural Stem Cell Progeny

et al. 2011

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Large-scale proliferation and multi-lineage differentiation capabilities make neural stem cells (NSCs) a promising renewable source of cells for therapeutic applications. However, the practical application for neuronal cell replacement is limited by heterogeneity of NSC progeny, relatively low yield of neurons, predominance of astrocytes, poor survival of donor cells following transplantation and the potential for uncontrolled proliferation of precursor cells. To address these impediments, we have developed a method for the generation of highly enriched immature neurons from murine NSC progeny. Adaptation of the standard differentiation procedure in concert with flow cytometry selection, using scattered light and positive fluorescent light selection based on cell surface antibody binding, provided a near pure (97%) immature neuron population. Using the purified neurons, we screened a panel of growth factors and found that bone morphogenetic protein-4 (BMP-4) demonstrated a strong survival effect on the cells in vitro, and enhanced their functional maturity. This effect was maintained following transplantation into the adult mouse striatum where we observed a 2-fold increase in the survival of the implanted cells and a 3-fold increase in NeuN expression. Additionally, based on the neural-colony forming cell assay (N-CFCA), we noted a 64 fold reduction of the bona fide NSC frequency in neuronal cell population and that implanted donor cells showed no signs of excessive or uncontrolled proliferation. The ability to provide defined neural cell populations from renewable sources such as NSC may find application for cell replacement therapies in the central nervous system.

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Maturation of Fetal Human Neural Xenografts in the Adult Rat Brain

Cited by 22 publications

References 40 publications

Survival and integration of transplanted postmitotic human neurons following experimental brain injury in immunocompetent rats

Survival and integration of transplanted postmitotic human neurons following experimental brain injury in immunocompetent rats

Synaptic transmission of neural stem cells seeded in 3-dimensional PLGA scaffolds

Purification of Immature Neuronal Cells from Neural Stem Cell Progeny

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