Measurement of adsorption isotherms by liquid chromatography

Jacobson, Jana; Frenz, John; Horváth, Csaba

doi:10.1016/s0021-9673(00)96140-5

Cited by 189 publications

(60 citation statements)

References 17 publications

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“…In most cases, aggregation alters the apparent values of these physical properties. Many researchers have used the frontal analysis (of breakthrough curves) to estimate equilibrium isotherm parameters (Huber and Gerritse, 1971 ;Jacobson et al, 1984). If reactions, such as aggregation, occur in these systems, the accuracy of frontal analysis may be greatly compromised.…”

Section: Effect Of Aggregation On Parameter Estimationmentioning

confidence: 99%

Effects of protein aggregation in isocratic nonlinear chromatography

et al. 1991

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Section: Effect Of Aggregation On Parameter Estimationmentioning

confidence: 99%

Effects of protein aggregation in isocratic nonlinear chromatography

et al. 1991

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“…1) of Eqs. (2) and (3) shows that, for a Langmuir-type isotherm, the migration velocity of a shock of concentration C is smaller than the migration velocity of concentration C on the rear part of the zone. Therefore, the shock continuously erodes and its velocity decreases.…”

Section: The Ideal Modelmentioning

confidence: 99%

“…The scientific interest for this type of chromatography had a strong impulse at the same time as fundamental works in separation science. Enlightening the behavior of solutes and separation media and changes caused by different experimental variables constituted the base for future developments in nonlinear chromatography.Chromatographic methods based on packed column for the determination of adsorption isotherms allowed to avoid some of the main drawbacks affecting static methods (e. g. the slowness and uncertainty in reaching equilibrium, the large amount of solute and adsorbent required) [2]. Success in preparative applications requires successful operations at high concentrations.…”

mentioning

confidence: 99%

Determination of adsorption isotherms by means of HPLC: Adsorption mechanism elucidation and separation optimization

Marchetti¹,

Cavazzini²,

Pasti³

et al. 2009

J of Separation Science

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The purpose of this review is to illustrate the most important techniques for isotherm determination by means of HPLC. Starting on the traditional Frontal Analysis approach, Frontal Analysis by Characteristic Point, Elution by Characteristic Point, Perturbation Method in its different applications will be considered to conclude with the most recent Inverse Method approach. Since many of these techniques are based on the fundamentals of nonlinear chromatography, a short overview of the theory of nonlinear chromatography is presented. Emphasis is given to the most recent applications of these techniques for pharmaceutical applications, characterization of binding mechanisms, bioaffinity studies, molecular and chiral recognition processes.

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“…Adsorption isotherms of BAEE and CLME were determined by frontal chromatography according to the method of Jacobson et al 9 The steric factor was determined from the breakthrough volume of nonlinear frontal chromatographic experiments. The characteristic charge was determined from the induced salt gradients produced during these experiments.…”

Section: Determination Of Adsorption Isotherms and Steric Mass Actionmentioning

confidence: 99%

Protected amino acids as novel low‐molecular‐weight displacers in cation‐exchange displacement chromatography

Kundu

Vunnum

Jayaraman

et al. 1995

Biotech & Bioengineering

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Although the ability to carry out simultaneous concentration and purification in a single displacement step has significant advantages for downstream processing of pharmaceuticals, a major impediment to the implementation of displacement chromatography has been the lack of suitable displacer compounds. An important recent advance in the state of the art of displacement chromatography has been the discovery that low-molecular-weight dendritic polymers can be successfully employed as displacers for protein purification in ion-exchange systems. In this article, protected amino acid esters (based on arginine and lysine) are shown to be useful displacers for protein purification in cation-exchange systems. A dynamic affinity plot is employed to evaluate the affinity of these low-molecular-weight compounds under dis-placement conditions. In contrast to large polyelectroyte displacers, the efficacy of these low-molecular-weight displacers was shown to be dependent on both the initial carrier salt concentration and the displacer concentration. In addition to the funcamental interest generated by low-molecular-weight displacers, it is likely that these displacers will have significant operatioal advantages as compared with large polyelectrolyte displacers. (c) 1995 John Wiley & Sons, Inc.

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Measurement of adsorption isotherms by liquid chromatography

Cited by 189 publications

References 17 publications

Effects of protein aggregation in isocratic nonlinear chromatography

Effects of protein aggregation in isocratic nonlinear chromatography

Determination of adsorption isotherms by means of HPLC: Adsorption mechanism elucidation and separation optimization

Protected amino acids as novel low‐molecular‐weight displacers in cation‐exchange displacement chromatography

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