Measurement of ethanol using fluorescence quenching

Sharma, Ashutosh; Quantrill, Nigel S. M.

doi:10.1016/0584-8539(94)80038-3

Cited by 12 publications

(12 citation statements)

References 12 publications

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“…The direct spectroscopic determination of NAD(P)H is based on the fact that the absorption/excitation band(s) of NAD(P)H are between 280–360 nm, with the maximum occurring at 340 ± 30 nm and the emission band at 460 ± 50 nm. Any absorption/emission band of NAD(P) + could not be detected [ 34 , 35 ].…”

Section: Introductionmentioning

confidence: 99%

“…A typical example with NAD + cofactor is alcohol dehydrogenase (ADH, EC 1.1.1.1), which can catalyze the reversible oxidation of alcohol to the corresponding aldehydes and ketones with the reduction of the nicotine adenine dinucleotide [ 35 , 36 ]. The oxidative reaction of ethanol to acetaldehyde coupled with the reduction of NAD + to NADH is catalyzed by the ADH enzyme: CH 3 CH 2 OH + NAD + → CH 3 CHO + NADH + H + …”

Section: Introductionmentioning

confidence: 99%

“…In addition, it can be also used for the determination of ethanol in food, alcoholic drinks, etc. Some examples are given elsewhere [ 35 , 37 , 38 , 39 , 40 , 41 ].…”

Section: Introductionmentioning

confidence: 99%

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Luminescent Sensor Based on Ln(III) Ternary Complexes for NAD(P)H Detection

Smrčka

Lubal

2020

Molecules

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Ln(III) complexes of macrocyclic ligands are used in medicinal chemistry, for example as contrast agents in MRI or radiopharmaceutical compounds, and in diagnostics using fluorescence imaging. This paper is devoted to a spectroscopic study of Ln(III) ternary complexes consisting of macrocyclic heptadentate DO3A and bidentate 3-isoquinolinate (IQCA) ligands. IQCA serves as an efficient antenna ligand, leading to a higher quantum yield and Stokes shift (250–350 nm for Eu, Tb, Sm, Dy in VIS region, 550–650 nm for Yb, Nd in NIR region). The shielding-quenching effect of NAD(P)H on the luminescence of the Ln(III) ternary complexes was investigated in detail and this phenomenon was utilized for the analytical determination of this compound. This general approach was verified through an enzymatic reaction during which the course of ethanol transformation catalyzed by alcohol-dehydrogenase (ADH) was followed by luminescence spectroscopy. This method can be utilized for selective and sensitive determination of ethanol concentration and/or ADH enzyme activity. This new analytical method can also be used for other enzyme systems coupled with NAD(P)H/NAD(P)+ redox pairs.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Luminescent Sensor Based on Ln(III) Ternary Complexes for NAD(P)H Detection

Smrčka

Lubal

2020

Molecules

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“…NADH absorbs ultraviolet (UV) excitation of 340 nm while NAD + does not. Previously, EtOH has been measured by use of NADH fluorescence [27][28][29]. NADH-dependent biosensors are not only useful for EtOH but also useful for many other substances such as glucose, acetaldehyde, formaldehyde, etc.…”

Section: Introductionmentioning

confidence: 99%

A NADH-dependent fiber-optic biosensor for ethanol determination with a UV-LED excitation system

Kudo

Sawai

Wang

et al. 2009

Sensors and Actuators B: Chemical

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“…NADH comes with the unique optical property that when it absorbs 340 nm UV light, it will release visible fluorescence while NAD + does not have same property [22]. Thus, the ethanol concentration is determined according to the fluorescence intensity emitted from the NADH [21,[23][24][25]. This kind of NADH-dependent optical Optical isopropanol biosensor using NADH-dependent secondary alcohol dehydrogenase (S-ADH) Po-Jen Chien et al…”

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confidence: 99%

Optical isopropanol biosensor using NADH-dependent secondary alcohol dehydrogenase (S-ADH)

Chien

Suzuki

et al. 2016

Talanta

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Isopropanol (IPA) is an important solvent used in industrial activity often found in hospitals as antiseptic alcohol rub. Also, IPA may have the potential to be a biomarker of diabetic ketoacidosis. In this study, an optical biosensor using NADH-dependent secondary alcohol dehydrogenase (S-ADH) for IPA measurement was constructed and evaluated. An ultraviolet light emitting diode (UV-LED, λ=340nm) was employed as the excitation light to excite nicotinamide adenine dinucleotide (NADH). A photomultiplier tube (PMT) was connected to a two-way branch optical fiber for measuring the fluorescence emitted from the NADH. S-ADH was immobilized on the membrane to catalyze IPA to acetone and reduce NAD(+) to be NADH. This IPA biosensor shows highly sensitivity and selectivity, the calibration range is from 500 nmol L(-1) to 1mmolL(-1). The optimization of buffer pH, temperature, and the enzyme-immobilized method were also evaluated. The detection of IPA in nail related cosmetic using our IPA biosensor was also carried out. The results showed that large amounts of IPA were used in these kinds of cosmetics. This IPA biosensor comes with the advantages of rapid reaction, good reproducibility, and wide dynamic range, and is also expected to use for clinical IPA detections in serum or other medical and health related applications.

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Measurement of ethanol using fluorescence quenching

Cited by 12 publications

References 12 publications

Luminescent Sensor Based on Ln(III) Ternary Complexes for NAD(P)H Detection

Luminescent Sensor Based on Ln(III) Ternary Complexes for NAD(P)H Detection

A NADH-dependent fiber-optic biosensor for ethanol determination with a UV-LED excitation system

Optical isopropanol biosensor using NADH-dependent secondary alcohol dehydrogenase (S-ADH)

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