Optical isopropanol biosensor using NADH-dependent secondary alcohol dehydrogenase (S-ADH)

Abstract

Isopropanol (IPA) is an important solvent used in industrial activity often found in hospitals as antiseptic alcohol rub. Also, IPA may have the potential to be a biomarker of diabetic ketoacidosis. In this study, an optical biosensor using NADH-dependent secondary alcohol dehydrogenase (S-ADH) for IPA measurement was constructed and evaluated. An ultraviolet light emitting diode (UV-LED, λ=340nm) was employed as the excitation light to excite nicotinamide adenine dinucleotide (NADH). A photomultiplier tube (PMT) was connected to a two-way branch optical fiber for measuring the fluorescence emitted from the NADH. S-ADH was immobilized on the membrane to catalyze IPA to acetone and reduce NAD(+) to be NADH. This IPA biosensor shows highly sensitivity and selectivity, the calibration range is from 500 nmol L(-1) to 1mmolL(-1). The optimization of buffer pH, temperature, and the enzyme-immobilized method were also evaluated. The detection of IPA in nail related cosmetic using our IPA biosensor was also carried out. The results showed that large amounts of IPA were used in these kinds of cosmetics. This IPA biosensor comes with the advantages of rapid reaction, good reproducibility, and wide dynamic range, and is also expected to use for clinical IPA detections in serum or other medical and health related applications.

Fluorometric Sniff-Cam (Gas-Imaging System) Utilizing Alcohol Dehydrogenase for Imaging Concentration Distribution of Acetaldehyde in Breath and Transdermal Vapor after Drinking

“…A glass gas outlet was placed on a backside of the ADH-immobilized mesh. Incidentally, it is reported that the environmental temperature affects enzyme activity and fluorescence intensity of NADH . Thus, environmental temperature was kept at 25 ± 1.5 °C for all experiments using an air conditioner.…”

“…Incidentally, it is reported that the environmental temperature affects enzyme activity and fluorescence intensity of NADH. 34 Thus, environmental temperature was kept at 25 ± 1.5 °C for all experiments using an air conditioner. Thus, the temperature did not affect the enzyme activity and fluorescence intensity of NADH.…”

Silicon Photomultiplier Current and Prospective Applications in Biological and Radiological Photonics

“…If light is the physical quantity transduced by the electronic component of the device, then we have an optical biosensor [46]. A SiPM can be integrated in a biosensor serving as the optical transducer and detecting small changes in absorbance, luminescence, polarization, or refractive index between reactants and products of a biological process [47,48]. Bio-luminescence and light scattering are optical phenomena used by biosensors that have proven most effective in the field of bioimaging research [49,50]; on the other hand, the collection of photoluminescence is very difficult due its extremely low intensity, typically in the order of a micro-lux [51,52].…”

Immobilization of alcohol dehydrogenase from Saccharomyces cerevisiae onto carboxymethyl dextran-coated magnetic nanoparticles: a novel route for biocatalyst improvement via epoxy activation

“…Because of its enantioselectivity, it is an excellent catalyst in the pharmaceutical industry, since it can produce high-value enantiopure drugs 75 , 76 . ADH also has great application for production of various starting materials and intermediates, and synthesis of chiral compounds, but mainly for regeneration of the coenzymes NAD(P) and NAD(P)H. It has been applied in biosensor technology, as well 77 – 80 . However, ADH has low stability, which limits its use in industrial applications 81 , owing to its high sensitivity to temperature and pH.…”