Measurement of Individual vs Total Antioxidants

Redox status of patients before cardiac surgery

“…Creatinine levels in patients examined in this study varied from 0.4 to 6.0 mg per deciliter (mean ± SD: 1.04 ± 0.56), with reference levels for healthy persons being 0.6 to 1.2 mg/dl for women and 0.7 to 1.3 mg/dl for men (internal reference values of UKGM, Giessen). Our data are in accordance with the results of Lamont et al [34], who found a correlation between TAOS and creatinine (0.256) and an even higher correlation with uric acid (0.526).…”

“…Levels for healthy individuals reported in other studies range from 719 ± 0.260 μM to 1,670 ± 130 μM [31,13,32], although they were determined with various methods that correlate only partially [33]. Measuring the antioxidative capacity of blood plasma can be useful because several interacting antioxidant components such as total protein, uric acid, bilirubin, carotinoids, tocopherols, ascorbic acid, and unknown components are assessed simultaneously [12,13,34,35]. However, it should be kept in mind that the ABTS radicals, which are quenched in the assay, do not resemble physiological radical species [36].…”

Oxidative stress and psoriasis: the effect of antitumour necrosis factor-α inhibitor treatment

“…In terms of participation of the individual components to the overall antioxidant system, calculated on the basis of the single individual concentration and the respective antioxidant stoichiometric coefficient, the main contributor to TAC is uric acid (40–55%), followed by thiol groups (10–24%), ascorbic acid (8–15%) and vitamin E (< 10%). The contribution of single antioxidants to the overall TAC leaves an unexplained percentage ranging from 20–40%, which might be accounted for by unknown components, unmeasured molecules (coenzyme Q10) and/or synergistic interactions 38 …”

Total Antioxidant Capacity in Children with Acute Appendicitis

“…Although several studies have demonstrated that total antioxidant capacity may be correlated with individual antioxidants including total protein, albumin, uric acid, and bilirubin, the overall antioxidant capacity may give more relevant biological information than that obtained by the measurement of individual parameters, as it takes account of the cumulative effect of all antioxidants present in plasma and body fluids [4,9]. A great variety of methods have been proposed for the assay of the total antioxidant capacity of organisms [1,6,9]. Antioxidant capacity is the number of moles of a given free radical scavenged by a test solution, independently of the capacity of any single antioxidant present in the mixture.…”