Measurement of macrophage adherence and spreading with weak electric fields

Kowolenko, Michael; Keese, Charles R.; Lawrence, David A.; Giæver, Ivar

doi:10.1016/0022-1759(90)90342-s

Cited by 59 publications

(45 citation statements)

References 29 publications

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“…As the cells attach and spread on the small electrodes, their effective area is partially blocked, and this results in an increase in impedance. We have used the device to detect the response of murine macrophages to biologically active compounds (38). In more recent work, we have used a spectrum of ac frequencies from 22 to 90,000 Hz.…”

Section: Resultsmentioning

confidence: 99%

Electrical method for detection of endothelial cell shape change in real time: assessment of endothelial barrier function.

Tiruppathì

Malik

Vecchio

et al. 1992

Proc. Natl. Acad. Sci. U.S.A.

408

320

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We have developed an electrical method to study endothelial cell shape changes in real time in order to examine the mechanisms of alterations in the endothelial barrier function. Endothelial shape changes were quantified by using a monolayer of endothelial cells grown on a small (10-3 cm2) evaporated gold electrode and measuring the changes in electrical impedance. Bovine pulmonary microvessel endothelial cells and bovine pulmonary artery endothelial cells were used to study the effects of a-thrombin on cell-shape dynamics by the impedance measurement. a-Thrombin produced a dose-dependent decrease in impedance that occurred within 0.5 m inin both cell types, indicative of retraction of endothelial cells and widening of interendothelial junctions because of "rounding up" of the cells. The a-thrombin-induced decrease in impedance persisted for -2 hr, after which the value recovered to basal levels. Pretreatment of endothelial cells with the protein kinase C inhibitor, calphostin C, or with 8-bromoadenosine 3',5'-cyclic monophosphate prevented the decreased impedance, suggesting that the endothelial cell change is modulated by activation of second-messenger pathways. The a-thrombin-induced decrease in impedance was in agreement with the previously observed increases in transendothelial albumin permeability and evidence of formation of intercellular gaps after a-thrombin challenge. The impedance measurement may be a valuable in vitro method for the assessment of mechanisms of decreased endothelial barrier function occurring with inflammatory mediators. Since the rapidly occurring changes in endothelial cell shape in response to mediators such as thrombin are mediated activation of second-messenger pathways, the ability to monitor endothelial cell dynamics in real time may provide insights into the signal-transduction events mediating the increased endothelial permeability.The vascular endothelium plays a central role in the maintenance of vascular homeostasis. Vascular endothelial cell monolayer functions as a barrier between the blood and interstitial compartments (1). A decrease in the barrier properties of vascular endothelium leads to tissue edema. Increased endothelial permeability to plasma proteins is the characteristic feature of many inflammatory conditions. Proinflammatory mediators such as a-thrombin, histamine, platelet-activating factor, and oxygen radicals have been shown to increase vascular endothelial permeability to macromolecules (2-8). However, the intracellular mechanisms by which these agents mediate the response remain unclear.The cultured endothelial cell monolayer grown on a porous filter has been used extensively to study the barrier function of endothelial cells (9, 10). We have examined in a series of studies the permeability-increasing properties of a-thrombin, a potent proinflammatory mediator (2-5, 11). Morphological studies indicated that a-thrombin causes endothelial cell retraction, which is believed to lead to intercellular gap formation and to the observed increases of the...

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Section: Resultsmentioning

confidence: 99%

Electrical method for detection of endothelial cell shape change in real time: assessment of endothelial barrier function.

Tiruppathì

Malik

Vecchio

et al. 1992

Proc. Natl. Acad. Sci. U.S.A.

408

320

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“…In the last two decades, ECIS sensing has been employed to monitor many types of cells, including fibroblasts, endothelial cells, cancer cells, etc. (Earley and Plopper, 2006;Kowolenko et al, 1990;Luong et al, 2001;Tiruppathi et al, 1992;Xiao et al, 2002). More recently, ECIS research turned to excitable cells, such as skeletal muscle cells (Aas et al, 2002) and cardiomyocytes .…”

Section: Introductionmentioning

confidence: 96%

Intervention of cardiomyocyte death based on real-time monitoring of cell adhesion through impedance sensing

Qiu¹,

Liao²,

Zhang³

2009

Biosensors and Bioelectronics

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“…Additionally, they demonstrated the applicability of the method in the study of cell adherence on protein coated surfaces, cultivating bone-marrow derived macrophages [138] and fibroblasts [139] on surfaces coated with fibronectin, gelatin and serum proteins. In the case of macrophages, the cells were activated by introducing heat-killed Listeria monocytogenes, lipopolysaccharide and interferon-g, which initiate biochemical processes, such as increased protein synthesis and Ca 2þ uptake, resulting in morphological changes [140].…”

Section: Impedance Measurements On Cells Adhering To a Growth Substratementioning

confidence: 99%

Chip Based Electroanalytical Systems for Cell Analysis

et al. 2008

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This review with 239 references has as its aim to give the reader an introduction to the kinds of methods used for developing microchip based electrode systems as well as to cover the existing literature on electroanalytical systems where microchips play a crucial role for nondestructive measurements of processes related to living cells, i.e., systems without lysing the cells. The focus is on chip based amperometric and impedimetric cell analysis systems where measurements utilizing solely carbon fiber microelectrodes (CFME) and other nonchip electrode formats, such as CFME for exocytosis studies and scanning electrochemical microscopy (SECM) studies of living cells have been omitted. Included is also a discussion about some future and emerging nano tools and considerations that might have an impact on the future of "nondestructive" chip based electroanalysis of living cells.

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Measurement of macrophage adherence and spreading with weak electric fields

Cited by 59 publications

References 29 publications

Electrical method for detection of endothelial cell shape change in real time: assessment of endothelial barrier function.

Electrical method for detection of endothelial cell shape change in real time: assessment of endothelial barrier function.

Intervention of cardiomyocyte death based on real-time monitoring of cell adhesion through impedance sensing

Chip Based Electroanalytical Systems for Cell Analysis

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