Measurement of naproxen in human plasma by chip‐based immunoaffinity capillary electrophoresis

Abstract

An electrokinetic immunoassay performed in a chip-based capillary electrophoresis system is described for the rapid measurement of naproxen in human plasma. The system employs a fluorescently labeled antibody to capture and detect the analyte of interest within a 5 min total assay time with an LOD of 0.025 microg/mL and a saturation level of 450 microg/mL. The system compared well with a conventional HPLC technique but was found to be much faster. Application of the electrokinetic assay to the study of patients with allergy to naproxen demonstrated increased concentrations of the drug extending past the predicted elimination half-life. The portability of the system and its ability to process up to 18 samples per hour makes it suitable for use in emergency room situations.

Development and Validation of Liquid Chromatography- Mass Spectroscopy/Mass Spectroscopy Method for Quantitative Analysis of Naproxen in Human Plasma after Liquid-Liquid Extraction

“…Representative chromatograms of an aqueous standard with internal standard mixture and plasma samples spiked with naproxen and IS were shown in Figures 2 and 3. Calibration curve of naproxen was linear over the concentration range of 500.1 ng/mL to 100028.5ng/mL for plasma which is as good as or superior to the previous methods reported [13,15].…”

“…Most of its therapeutic activity is probably mediated through prostaglandin synthesis inhibition [6][7][8]. Several chromatographic methods have been reported for determination of naproxen in raw material [9], tablets [10][11][12], plasma [13][14][15], urine [16], intestinal perfusion samples [17] and pharmaceutical preparations [18][19][20].…”

A Validated Stability-Indicating UHPLC Method for Determination of Naproxen and Its Related Compounds in Bulk Drug Samples

“…Naproxen is cited in the European Pharmacopoeia to have contamination by compounds A, B, D, E [5,6] and Imp-C is likely to be present in the preparation of Naproxen by using asymmetric hydrogenation step process [7]. A survey on literature reveals that many chromatographic methods have been reported for the determination of Naproxen in raw material [8], tablets [9,10], plasma [11][12][13], urine [14], intestinal perfusion samples [15] and pharmaceutical preparations [16]. However, no stability indicating UHPLC method is reported for estimation of related compounds and assay of naproxen from bulk drug samples.…”

An Ultra-Sensitive and Selective LC-UV Method for the Simultaneous Determination of Pravastatin, Diltiazem, Naproxen Sodium and Meloxicam in API, Pharmaceutical Formulations and Human Serum

“…Different techniques have been reported for the determination of NAP i.e. chip-based immunoaffinity capillary electrophoresis [20], HPLC [21], HPTLC [22], LC-MS/MS [23] and GC/MS [24]. For the determination of MEL, spectrophotometric [25], Voltammetric [26], fluorimetric [27], electrochemical [28], HPLC [29] and capillary electrophoresis [30] are reported.…”