Measurement of plasma 25-hydroxyvitamin D2, 25-hydroxyvitamin D3 and 3-epi-25-hydroxyvitamin D3 in population of patients with cardiovascular disease by UPLC-MS/MS method

Abouzid, Mohamed; Karaźniewicz−Łada, Marta; Pawlak, Kornel; Burchardt, Paweł; Kruszyna, Łukasz; Główka, Franciszek

doi:10.1016/j.jchromb.2020.122350

Cited by 18 publications

(16 citation statements)

References 31 publications

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“…Written informed consent was obtained from each participant. In addition, 25(OH)D2, 25(OH)D3 and 3-epi-25(OH)D3 concentrations were assessed using the validated UPLC-MS/MS method [ 29 ]. Briefly, 200 μL of patients’ plasma was mixed with 20 μL of methanol and 20 μL of the internal standard, D6-25-hydroxyvitamin D3.…”

Section: Methodsmentioning

confidence: 99%

“…Recovery of the analytes from plasma samples were 85–107%. The inter- and inter-day precision was in the range of 4–18%, while accuracy of the method was 86–115% [ 29 ]. The applied UPLC-MS/MS method enabled for separating 25(OH)D2 and 25(OH)D3 from 3-epi-25(OH)D3, which allowed for avoiding overestimation of the 25(OH)D3 concentration in the studied group of patients with CVD.…”

Section: Methodsmentioning

confidence: 99%

“…According to Strathmann [ 28 ], 3% of adults would be misclassified as sufficient if 3-epi-25(OH)D3 was included in the quantification of 25(OH)D3. Moreover, recent research shows that the presence of the C-3 epimers may constitute up to 50% of the 25(OH)D content in adults [ 29 ]. Because the clinical impact of 3-epi-25(OH)D3 remains ambiguous, many studies are trying to measure it in various situations such as in pregnancy [ 30 ], chronic liver disease [ 31 ], and thyroid disorders [ 32 ].…”

Section: Introductionmentioning

confidence: 99%

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Vitamin D Receptor Gene Polymorphism and Vitamin D Status in Population of Patients with Cardiovascular Disease—A Preliminary Study

et al. 2021

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The association between vitamin D receptor (VDR) polymorphism and the risk of cardiovascular diseases (CVD) remains unclear. This study aimed to assess a relationship between the VDR genotypes, plasma concentrations of vitamin D metabolites, and the occurrence of cardiovascular and metabolic disorders. Fifty-eight patients treated for various cardiological afflictions were included. Identification of VDR polymorphisms: ApaI, TaqI, BsmI, and FokI were carried out using the PCR-RFLP method. Plasma concentrations of 25-hydroxyvitamin-D2, 25-hydroxyvitamin-D3, and 3-epi-25-hydroxyvitamin D3 were assessed by the UPLC-MS/MS method. Lower incidence of BsmI AA genotype in the studied patients was observed compared with healthy controls, but the difference was insignificant. Among patients with the TT genotype, frequency of hypertension was higher than among carriers of other ApaI genotypes (p < 0.01). In addition, carriers of the TT ApaI, TC TaqI, and GA BsmI genotypes had an increased risk of obesity, while the presence of the FokI TT genotype was associated with a higher incidence of heart failure and hypertension. In conclusion, the BsmI AA genotype can be protective against CVD, but this observation needs study on a larger group of patients. Particular VDR genotypes were associated with 25-hydroxyvitamin-D levels, and the mechanism of this association should be further investigated.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Vitamin D Receptor Gene Polymorphism and Vitamin D Status in Population of Patients with Cardiovascular Disease—A Preliminary Study

et al. 2021

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“…Recovery of 25(OH)D 2 and 25(OH)D 3 from human plasma was about 70%. The analytes proved to be stable during the storage conditions and sample pretreatment [ 27 ].…”

Section: Methodsmentioning

confidence: 99%

Differences in the Concentration of Vitamin D Metabolites in Plasma Due to the Low-Carbohydrate-High-Fat Diet and the Eastern European Diet—A Pilot Study

et al. 2021

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Vitamin D deficiency is a global problem with many health consequences, and it is currently recommended to supplement vitamin D. Change of diet should also be considered to ensure adequate vitamin D in the human body. The aim of this study was to assess the concentration of vitamin D metabolites in two different groups: one group on the low-carbohydrate-high-fat (LCHF) diet and the other group on the Eastern European (EE) diet. In the first stage, 817 participants declaring traditional EE diet or LCHF diet were investigated. Nutrition (self-reported 3-day estimated food record) and basic anthropometric parameters were assessed. After extra screening, 67 participants on the EE diet and 41 on the LCHF diet were qualified for the second stage. Plasma 25-hydroxycholecalciferol (25(OH)D3) and (25(OH)D2) concentration was measured by the validated HPLC—MS/MS method. Plasma 25(OH)D3 concentration was significantly higher in the group on the LCHF diet (34.9 ± 15.9 ng/mL) than in the group on the EE diet (22.6 ± 12.1 ng/mL). No statistical differences were observed in plasma 25(OH)D2 concentration between the study groups (p > 0.05). Women had a higher plasma 25(OH)D2 concentration than men regardless of diet type. The LCHF diet had a positive influence on plasma vitamin D concentration. However, long-term use of the LCHF diet remains contentious due to the high risk of cardiovascular disease. This study confirmed that the type of diet influences the concentration of vitamin D metabolites in the plasma.

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“…Recently, interest in the other forms and metabolites of vitamin D has arisen. The measurement of 25(OH)D using standard chromatographic methods may result in overestimated values because of the presence of the C-3 epimers, which may constitute up to 50% of the 25(OH)D content in adults [36,37]. Clinically, this issue is particularly important in estimating vitamin D status in infants where levels of the epimeric form may be higher than concentrations of 25(OH)D [38].…”

Section: Hplc-ms/msmentioning

confidence: 99%

Clinical Significance of Analysis of Vitamin D Status in Various Diseases

et al. 2020

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Vitamin D plays a role not only in the proper functioning of the skeletal system and the calcium-phosphate equilibrium, but also in the immune system, the cardiovascular system and the growth and division of cells. Although numerous studies have reported on the analysis of vitamin D status in various groups of patients, the clinical significance of measurements of vitamin D forms and metabolites remains ambiguous. This article reviews the reports analyzing the status of vitamin D in various chronic states. Particular attention is given to factors affecting measurement of vitamin D forms and metabolites. Relevant papers published during recent years were identified by an extensive PubMed search using appropriate keywords. Measurement of vitamin D status proved to be a useful tool in diagnosis and progression of metabolic syndrome, neurological disorders and cancer. High performance liquid chromatography coupled with tandem mass spectrometry has become the preferred method for analyzing the various forms and metabolites of vitamin D in biological fluids. Factors influencing vitamin D concentration, including socio-demographic and biochemical factors as well as the genetic polymorphism of the vitamin D receptor, along with vitamin D transporters and enzymes participating in vitamin D metabolism should be considered as potential confounders of the interpretation of plasma total 25(OH)D concentrations.

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Measurement of plasma 25-hydroxyvitamin D2, 25-hydroxyvitamin D3 and 3-epi-25-hydroxyvitamin D3 in population of patients with cardiovascular disease by UPLC-MS/MS method

Cited by 18 publications

References 31 publications

Vitamin D Receptor Gene Polymorphism and Vitamin D Status in Population of Patients with Cardiovascular Disease—A Preliminary Study

Vitamin D Receptor Gene Polymorphism and Vitamin D Status in Population of Patients with Cardiovascular Disease—A Preliminary Study

Differences in the Concentration of Vitamin D Metabolites in Plasma Due to the Low-Carbohydrate-High-Fat Diet and the Eastern European Diet—A Pilot Study

Clinical Significance of Analysis of Vitamin D Status in Various Diseases

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