Cereal Chem. 91(4): [406][407][408][409][410][411][412][413] Distillers dried grains with solubles (DDGS) is the main coproduct of the U.S. fuel ethanol industry and has significantly impacted the livestock feed markets in recent years. Particle agglomeration and subsequent flowability problems during storage and transport are often a hindrance, a nuisance, and expensive. This paper aims at characterizing the glass transition (T g ) and sticky point (T s ) temperatures of DDGS samples prepared with varying condensed distillers solubles (CDS) levels (10, 15, and 20%, wb), drying temperatures (100, 200, and 300°C), and moisture contents (0, 10, and 20%, db), and it discusses implications on DDGS flowability behavior. Distillers wet grains were combined with specified levels of CDS and dried in a convection-style laboratory oven to produce DDGS.Subsequently, predetermined amounts of water were added to the DDGS to achieve desired moisture content levels. To determine T g (°C), a differential scanning calorimeter was used, whereas T s (°C) was determined through a novel technique with a rheometer. Results indicated high correlations between observed T s and observed T g (R 2 = 0.87) data for DDGS samples. Also, the empirical model for predicted T g = f(drying temperature, CDS level, and moisture content) based on the Gordon-Taylor model showed favorable R 2 (0.74). Stickiness of DDGS increased with an increase in moisture content, indicating flow problems resulting from moisture. It was found that drying temperatures and CDS levels each had significant effects on T g and T s as well.Distillers dried grains with solubles (DDGS) is the primary coproduct from the corn-based fuel ethanol industry, which is mainly from dry-grind ethanol production plants across the Midwestern United States. It is commonly used as livestock feed for cattle, swine, and poultry. DDGS typically contains 86-93% (db) dry matter, 26-34% (db) crude protein, 3-13% (db) fat, and essential amino acids such as methionine, leucine, arginine, and threonine (Speihs et al 2002;Rosentrater and Muthukumarappan 2006). There has been enormous growth in the bioethanol industry in the last decade because of strong interest in alternative fuels, and therefore there is now substantial DDGS production. The production of DDGS is projected to have grown to nearly 40 million metric tons during 2013.Like many agricultural and food materials, DDGS is hygroscopic in nature (i.e., it can absorb moisture from the surrounding atmosphere). Thus, DDGS particles may tend to agglomerate to form solidified masses (i.e., caking) by absorbing water or because of temperature differences. Caking, or hardening, of DDGS leads to difficulties during unloading from rail cars, bins, and other storage vessels, and it can result in economic losses to break up the agglomerates and discharge the storage vessels (Rock and Schwedes 2005). Stickiness in particles (or the caking phenomenon) causes poor flowability in the material and, thus, makes it less efficient to handle during d...