Measurement of Surface Antigen by Specific Bacterial Adherence and Scanning Electron Microscopy (SABA/SEM) in Cells Infected by Vesiculovirus ts Mutants

Pringle, C. R.; Parry, J.E.

doi:10.1099/0022-1317-59-1-207

Cited by 7 publications

(1 citation statement)

References 13 publications

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“…Furthermore, there is now compelling evidence for more sporadic recombination in a variety of positive-sense RNA viruses from animals (Ball, 1997;Lai, 1992;Liao & Lai, 1992;Strauss & Strauss, 1997;, plants (Aaziz & Tepfer, 1999;Roossinck, 1997;Simon & Bujarski, 1994) and bacteria (Chetverin, 1997;Mindich, 1996). In contrast, there are few reports of recombination (aside from reassortment) in negative-sense RNA viruses, which has led some authors to suggest that these viruses are characterized by low rates of recombination (Pringle & Parry, 1982). To date, evidence for recombination has only been documented in some ambisense arenaviruses (Archer & Rico-Hesse, 2002;Charrel et al, 2001), hantaviruses (Klempa et al, 2003;Sibold et al, 1999;Sironen et al, 2001) and Influenza A virus (Gibbs et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

Phylogenetic analysis reveals a low rate of homologous recombination in negative-sense RNA viruses

Chare

Gould

Holmes

2003

Journal of General Virology

256

191

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Recombination is increasingly seen as an important means of shaping genetic diversity in RNA viruses. However, observed recombination frequencies vary widely among those viruses studied to date, with only sporadic occurrences reported in RNA viruses with negative-sense genomes. To determine the extent of homologous recombination in negative-sense RNA viruses, phylogenetic analyses of 79 gene sequence alignments from 35 negative-sense RNA viruses (a total of 2154 sequences) were carried out. Powerful evidence was found for recombination, in the form of incongruent phylogenetic trees between different gene regions, in only five sequences from Hantaan virus, Mumps virus and Newcastle disease virus. This is the first report of recombination in these viruses. More tentative evidence for recombination, where conflicting phylogenetic trees were observed (but were without strong bootstrap support) and/or where putative recombinant regions were very short, was found in three alignments from La Crosse virus and Puumala virus. Finally, patterns of sequence variation compatible with the action of recombination, but not definitive evidence for this process, were observed in a further ten viruses: Canine distemper virus, Crimean-Congo haemorrhagic fever virus, Influenza A virus, Influenza B virus, Influenza C virus, Lassa virus, Pirital virus, Rabies virus, Rift Valley Fever virus and Vesicular stomatitis virus. The possibility of recombination in these viruses should be investigated further. Overall, this study reveals that rates of homologous recombination in negative-sense RNA viruses are very much lower than those of mutation, with many viruses seemingly clonal on current data. Consequently, recombination rate is unlikely to be a trait that is set by natural selection to create advantageous or purge deleterious mutations.

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Section: Introductionmentioning

confidence: 99%

Phylogenetic analysis reveals a low rate of homologous recombination in negative-sense RNA viruses

Chare

Gould

Holmes

2003

Journal of General Virology

256

191

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Molecular Epidemiology, Genomics, and Phylogeny of Crimean-Congo Hemorrhagic Fever Virus

Hewson¹

2007

Crimean-Congo Hemorrhagic Fever

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Fixed-cell immunoperoxidase technique for the study of surface antigens induced by the coronavirus of transmissible gastroenteritis (TGEV)

Bernard

Lantier

1991

Veterinary Microbiology

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An immunoperoxidase technique performed on the TGEV-infected cells was developed for detection of virus-induced antigens. The presence of M antigen of TGEV on the surface of infected cells was demonstrated by this technique. This finding is in contrast to the M protein of murine hepatitis coronavirus which migrates to the Golgi apparatus but is not transported to the plasma membrane. The time course of appearance M and S antigens on the surface of TGEV-infected cell can be studied by this technique.

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Measurement of Surface Antigen by Specific Bacterial Adherence and Scanning Electron Microscopy (SABA/SEM) in Cells Infected by Vesiculovirus ts Mutants

Cited by 7 publications

References 13 publications

Phylogenetic analysis reveals a low rate of homologous recombination in negative-sense RNA viruses

Phylogenetic analysis reveals a low rate of homologous recombination in negative-sense RNA viruses

Molecular Epidemiology, Genomics, and Phylogeny of Crimean-Congo Hemorrhagic Fever Virus

Fixed-cell immunoperoxidase technique for the study of surface antigens induced by the coronavirus of transmissible gastroenteritis (TGEV)

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