Measurement of whole body acetate turnover in healthy subjects with stable isotopes

Catherine, Simoneau; Pouteau, Etienne; Maugeais, P.; Marks, Lisa; Ranganathan, S.; Champ, Martine; Krempf, Michel

doi:10.1002/bms.1200230707

Cited by 22 publications

(25 citation statements)

References 9 publications

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“…Measurement of plasma acetate enrichment was previously described (16,24). Briefly, the proteins of plasma samples containing an internal standard (D 3-acetate) were precipitated by sulfosalicylic acid (10 mg).…”

Section: Acetate Analysismentioning

confidence: 99%

Colonic fermentation from lactulose inhibits lipolysis in overweight subjects

Ferchaud‐Roucher

Pouteau²,

Piloquet³

et al. 2005

American Journal of Physiology-Endocrinology and Metabolism

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One of the strategies to prevent insulin resistance is to reduce circulating free fatty acids (FFA). The aim of this study is to assess the effect of an oral lactulose load on fatty acid metabolism in overweight subjects. Eight overweight subjects received a primed constant intravenous infusion of [1-(13)C]acetate and of [1,1,2,3,3-(2)H(5)]glycerol for 9 h. After 3 h of tracer infusion, patients ingested 30 g lactulose, or saline solution. Arterialized blood samples were collected every 20 min. Basal plasma concentrations of acetate were similar before and between oral treatments as well as glycerol and FFA concentrations. Plasma acetate turnover was 11.4 +/- 2.4 vs. 10.7 +/- 1.4 micromol.kg(-1).min(-1) [not significant (NS)], and plasma glycerol turnover was 3.8 +/- 0.4 vs. 4.8 +/- 1.9 micromol.kg(-1).min(-1) (NS). After lactulose ingestion, acetate concentration increased twofold and then decreased to baseline. Acetate turnover rate increased to 15.5 +/- 2.2 micromol.kg(-1).min(-1) after lactulose treatment, whereas it was unchanged after saline treatment (10.3 +/- 2.2 micromol.kg(-1).min(-1), P < or = 0.0001). In contrast, FFA concentrations decreased significantly after lactulose ingestion and then increased slowly. Glycerol turnover decreased after lactulose ingestion compared with saline, 2.8 +/- 0.4 vs. 3.5 +/- 0.3 micromol.kg(-1).min(-1) (P < or = 0.05). A significant negative correlation was found between glycerol and acetate turnover after lactulose treatments (r = -0.78, P < or = 0.02). These results showed in overweight subjects a short-term decrease in FFA level and glycerol turnover after lactulose ingestion related to a decrease of lipolysis in close relationship with an increase of acetate production.

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Section: Acetate Analysismentioning

confidence: 99%

Colonic fermentation from lactulose inhibits lipolysis in overweight subjects

Ferchaud‐Roucher

Pouteau²,

Piloquet³

et al. 2005

American Journal of Physiology-Endocrinology and Metabolism

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“…10 A few notable studies have measured exogenous VFA production either by measuring the portal appearance of VFAs 11 or by isotope dilution techniques. [12][13][14][15] Many studies have been conducted in animals using stable and radioisotope techniques to measure VFA production and these have been largely successful because of invasive sampling techniques and the relatively high concentration of VFAs in the large intestine/ rumen. 16 However, it is not appropriate to extrapolate animal data to humans because of nutritional, anatomical, physiological, and microbiological differences.…”

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confidence: 99%

A streamlined approach to the analysis of volatile fatty acids and its application to the measurement of whole‐body flux

Morrison

Cooper

Waldron

et al. 2004

Rapid Comm Mass Spectrometry

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Volatile fatty acids (VFAs) are produced in the human colon by the bacterial breakdown of carbohydrates that escape digestion and absorption in the small intestine. They have important local and systemic effects on gastrointestinal and nutritional functions. Measuring their production is difficult because of inaccessibility of sampling sites and low circulating concentrations. Stable isotope tracer techniques are a way to measure VFA production but require measurement of isotope dilution in blood and other biological fluids. We have developed a streamlined and robust method to measure the concentration and enrichment of [(2)H]-labelled VFAs by gas chromatography/mass spectrometry (GC/MS) and [(13)C]-labelled VFAs by gas chromatography/combustion/isotope ratio mass spectrometry (GC/C/IRMS). Both types of analysis were carried out on the same samples allowing multiple tracer studies to be conducted. Good accuracy and repeatability were found for GC/MS analysis of [(2)H]-labelled VFAs. Careful handling of the background contribution, especially acetate, allowed quantitation of concentration and enrichment within the analysis. GC/C/IRMS analysis of [(13)C] VFAs was also achieved with good accuracy and repeatability. This methodology was used to determine whole-body acetate production in two subjects using multiple tracers ([(2)H(3)]- and [1-(13)C]acetate) and blood and urine sampling. Whole-body acetate flux was similar when measured either with [(2)H(3)]- or [1-(13)C]acetate, and when flux was determined from plasma or urine tracer enrichment. This new method will permit rapid and accurate measurement of VFA flux using [(2)H]- and/or [(13)C]-labelled VFAs as tracers. Measurements of the contribution of colonic VFA production to whole-body VFA flux are now possible.

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“…It is estimated that the level of the acetate ion in humans is about 50-60 µmol/l (3.0-3.6 mg/l) in plasma and 116 µmol/l (7 mg/l) in cerebrospinal fluid [3], [4]. Daily turnover of the acetate ion in humans is estimated to be about 7.5 µmol/kg/min representing some 45 g/day [3], [5]. Estimations of the daily intake of acetic acid vary from about 1 gram [3], [6].…”

Section: Introductionmentioning

confidence: 99%

Determination of Formic and Acetic Acids in Indoor Air

Diodiu¹,

Bucur²

2015

Simi 2015

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A method for the determination of formic and acetic acids was realized and validated in this work. The separation of the two organic acids was optimized in 10 minutes on an IonPac AS-18 column coupled with precolumns, isocratic mode, 0.250ml/min flow and a mobile phase consisting in KOH 10mM. The quantification of the interested compounds was done by external standard. Then, the method was validated by analyzing selectivity, linearity, limit of detection, limit of quantification, precision and accuracy. The validated method can be described as a simple and also low cost method for the determination of formic and acetic acid in air samplers.

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Measurement of whole body acetate turnover in healthy subjects with stable isotopes

Cited by 22 publications

References 9 publications

Colonic fermentation from lactulose inhibits lipolysis in overweight subjects

Colonic fermentation from lactulose inhibits lipolysis in overweight subjects

A streamlined approach to the analysis of volatile fatty acids and its application to the measurement of whole‐body flux

Determination of Formic and Acetic Acids in Indoor Air

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